SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP006465
Component protein type	TssK
T6SS ID (Type)	T6SS00613 (Type i1)
Strain	Pseudomonas aeruginosa M18
Replicon	chromosome
Sequence	Protein sequence (444 a.a.); Nucleotide sequence (1332 bp)
Reference	[1] Wei X, Huang X, Tang L, et al. Global control of GacA in secondary metabolism, primary metabolism, secretion systems, and motility in the rhizobacterium Pseudomonas aeruginosa M18. J Bacteriol. 2013 Aug;195(15):3387-400. doi: 10.1128/JB.00214-13. Epub 2013 May 24. PMID: 23708134

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PAM18_RS17325
Coordinate (Strand)	3761699..3763030 (-)
NCBI ID	WP_003097790.1
RefSeq	NC_017548
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasE	PF05936.14	3.5e-142	18..441

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 444 a.a.

						>T6CP006465 NC_017548:c3763030-3761699 [Pseudomonas aeruginosa M18] [TssK]
MNVHKIVWQEGMLLRPQHLQHNDRYYDNQMKARTQLLGSYNWGFLALELDRQFLNMGKLVISQASGVLPDGSLFELGGNT
EPLALDVPPNTSNVPIYLALPLVTGNHIEARRPEQAEVLARYTAYDLEVADSNAGEDSMVQVSCARPDFRLLLGEQQSDQ
AYVKLKVAVVLDTTPDGVISLDPDFVPSYLHAHASAYLLSCLKEVISMLGHRGDTIAERIRSNGKVGGAEVGDFMMLQLI
NRTELLLRHYLGLEQVHPEQLYRVLLTMLGDLATFGSESKRPRLDSRYQHSDQGASFRRLMEAIRQVLSMVLEQHAIELP
LQARQYGILVSPLHDHKLLGSASFVLAASANCESEELRQRLPAHLKVGAVEHIRQLVNLHLPGIRVKPLPVAPRQIPFHS
NKTYFILEPNAEDMAQLERSGGFAFHVSGEFSELELKFWAIRN*					

Nucleotide sequence: 1332 bp

						>T6CP006465 NC_017548:c3763030-3761699 [Pseudomonas aeruginosa M18] [TssK]
ATGAACGTGCACAAGATCGTCTGGCAGGAAGGCATGCTGCTGCGCCCGCAGCACCTGCAACACAACGACCGCTACTACGA
CAACCAGATGAAGGCCCGGACCCAGTTGCTGGGCAGCTACAACTGGGGCTTCCTCGCCCTGGAGCTGGACCGCCAGTTCC
TCAACATGGGCAAGCTGGTGATCAGCCAGGCCTCCGGCGTATTGCCCGACGGCAGCCTGTTCGAACTCGGCGGCAACACC
GAGCCGCTCGCCCTCGACGTGCCGCCCAATACCTCGAACGTGCCGATCTACCTGGCCCTGCCGCTGGTCACCGGCAACCA
CATCGAGGCGCGCCGGCCGGAGCAGGCCGAGGTCCTGGCGCGCTATACCGCCTATGACCTGGAGGTGGCGGACTCCAACG
CCGGCGAGGATTCCATGGTGCAGGTCAGCTGCGCGCGGCCGGATTTTCGCCTGCTGCTCGGCGAGCAGCAGAGCGACCAG
GCCTACGTCAAACTGAAGGTGGCGGTGGTGCTGGATACCACGCCGGACGGGGTGATCAGCCTCGACCCCGACTTCGTGCC
GAGCTACCTCCACGCCCACGCATCCGCCTACCTGCTGTCCTGCCTGAAGGAGGTGATCAGCATGCTCGGCCATCGCGGCG
ACACCATCGCCGAGCGTATCCGGTCCAATGGCAAGGTCGGCGGCGCGGAGGTCGGCGATTTCATGATGCTGCAACTGATC
AATCGCACCGAGTTGCTGCTGCGCCACTACCTGGGATTGGAGCAGGTGCATCCGGAGCAGTTGTACCGGGTGCTGCTGAC
CATGCTCGGCGACCTCGCCACCTTCGGCAGCGAGAGCAAGCGTCCGCGCCTGGACAGCCGTTACCAGCACAGCGACCAGG
GCGCCAGCTTCCGCAGGCTGATGGAGGCGATCCGCCAGGTCCTGTCGATGGTGCTCGAGCAGCATGCCATCGAACTGCCT
CTCCAGGCCCGCCAGTACGGCATCCTGGTCTCGCCCCTGCACGACCACAAGCTGCTCGGCAGCGCCTCGTTCGTGCTCGC
CGCCAGTGCCAATTGCGAGTCGGAGGAACTGCGCCAGCGGCTGCCCGCGCATCTCAAGGTGGGTGCGGTCGAGCACATTC
GCCAACTGGTCAACCTGCACCTGCCGGGGATCCGGGTCAAGCCGCTGCCGGTGGCGCCGCGGCAGATCCCGTTCCACTCC
AACAAGACCTATTTCATTCTCGAACCCAACGCCGAGGACATGGCCCAGCTGGAGCGCTCCGGCGGTTTCGCGTTCCACGT
ATCCGGCGAGTTCTCCGAGCTTGAACTGAAATTCTGGGCCATCAGGAACTGA					