Detailed information of component protein
Summary
Component ID | T6CP006461 |
Component protein type | TssM |
T6SS ID (Type) | T6SS00612 (Type i4a) |
Strain | Pseudomonas aeruginosa M18 |
Replicon | chromosome |
Sequence | Protein sequence (1272 a.a.); Nucleotide sequence (3816 bp) |
Reference |
External database links
Locus tag (Gene) | PAM18_RS13785 |
Coordinate (Strand) | 2981490..2985305 (+) |
NCBI ID | WP_014602902.1 |
RefSeq | NC_017548 |
Uniprot ID | - |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related_N | PF14331.8 | 2.9e-48 | 115..352 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
- | - |
Protein sequence: 1272 a.a.
>T6CP006461 NC_017548:2981490-2985305 [Pseudomonas aeruginosa M18] [TssM]
MSGATLFMLVLLLVLLALLLGALGWWWRTRGGTEIRSFYAAVRQMEREQGWQGRYEAPWLLMLGNETEGEQLCSTWRLLP
VARPAWFGRWWSDGEGAILLVPESVFLPDEGLRRQSGAWLRLLRLFLRLRGRRALDGVVWNIPMARLQDGEQAANLGLAA
RRRYVELTQRLGLSLPVYVVITGLEDLPGFQELLAALPEEARERALGWSSPFAAEAAWQSRWCEQALEEITATLTESIVE
LGTLRGQVDNELYCLPPRLESLRGSLQALLEPVFQGNARGEAPRFRGLYLSGSEAAGAAADEVLPAVDAPRRRSSFASQL
WARRILAEEGLAQAVPRILQLRQRWQRGIGLAALCLCLLWGGLMTWVWRDALRDAGELSQLLHGASERYQPLDDDTRRAA
QVRQNVQAWWQLVSQAPRWRFTSLAFPSSWFSSLDARIDNAYRRVSERLLVRPLRSLLEGEASDLRAIRSDGQPGLKEGD
DPSQWKDYLAAKDLVARATTLERHNRLFAQAIDNRRTPLDDLLQLSNDALGSSYNAGSLARLAYYNRTLFAERPADLAAL
DLGRVAATAADNFHDLMARWLNAYFLTDSLERTGNALTQELAQLGQERDATAAQLLGIGELINHLQQQVNRINLIWGNGV
GQDLVPGYQNLLKSAQQSSLLGNRVEELQNLTVQLQQQFRSEWIAPPEAAGDGLLVKQGATLKLADDLLGLKRAIDDLKT
KDFVSVALADKTLADHSLLSIDDIGVTQALSFFNDFTGYYESTLPSLNPRYRYSVLHAAASAATEAMWQSLGPRSRSLAS
RNASRFDVQVKQVQVLQAALLELQDLQAGARLLLSLNALAVADIGQALRDIDEQAVLREPVDFSRWSGAPNFGLQMFRAQ
DSAELKQSLNSQFNAMAAVAERHAPALEWLQAQQSSLATADYNAFVRFSALNAELQKFKADNPASTAAQLAKLVGNDFNQ
MDIGSCADILNGVLLPSSRSDLATRLVDLRQGALGRCQSLQQQQAAQAWKDLADYFNQYLAGRFPFAYSLEAADAEPGRV
RHLLKLMETRLPQVREGLAQVRSPDLPAAEDFVRRLEQAQRWLGPLFERDKSGLLGVALDIDWRSDRSLERGADQVIAWS
LYSGDQESRFPGAQDKGLTWNVGDPLKIMLRWAKNGSQRPADDPRQASLAVADLEAGWSYQGSWALLRMMRAHFSRQRPP
NVDYTEFPLVLQLPVYAPYSPENEARMFLRLSLMSLGGKTPLSIQPLPVRAPQSPFATLLPATVASTGGTP*
MSGATLFMLVLLLVLLALLLGALGWWWRTRGGTEIRSFYAAVRQMEREQGWQGRYEAPWLLMLGNETEGEQLCSTWRLLP
VARPAWFGRWWSDGEGAILLVPESVFLPDEGLRRQSGAWLRLLRLFLRLRGRRALDGVVWNIPMARLQDGEQAANLGLAA
RRRYVELTQRLGLSLPVYVVITGLEDLPGFQELLAALPEEARERALGWSSPFAAEAAWQSRWCEQALEEITATLTESIVE
LGTLRGQVDNELYCLPPRLESLRGSLQALLEPVFQGNARGEAPRFRGLYLSGSEAAGAAADEVLPAVDAPRRRSSFASQL
WARRILAEEGLAQAVPRILQLRQRWQRGIGLAALCLCLLWGGLMTWVWRDALRDAGELSQLLHGASERYQPLDDDTRRAA
QVRQNVQAWWQLVSQAPRWRFTSLAFPSSWFSSLDARIDNAYRRVSERLLVRPLRSLLEGEASDLRAIRSDGQPGLKEGD
DPSQWKDYLAAKDLVARATTLERHNRLFAQAIDNRRTPLDDLLQLSNDALGSSYNAGSLARLAYYNRTLFAERPADLAAL
DLGRVAATAADNFHDLMARWLNAYFLTDSLERTGNALTQELAQLGQERDATAAQLLGIGELINHLQQQVNRINLIWGNGV
GQDLVPGYQNLLKSAQQSSLLGNRVEELQNLTVQLQQQFRSEWIAPPEAAGDGLLVKQGATLKLADDLLGLKRAIDDLKT
KDFVSVALADKTLADHSLLSIDDIGVTQALSFFNDFTGYYESTLPSLNPRYRYSVLHAAASAATEAMWQSLGPRSRSLAS
RNASRFDVQVKQVQVLQAALLELQDLQAGARLLLSLNALAVADIGQALRDIDEQAVLREPVDFSRWSGAPNFGLQMFRAQ
DSAELKQSLNSQFNAMAAVAERHAPALEWLQAQQSSLATADYNAFVRFSALNAELQKFKADNPASTAAQLAKLVGNDFNQ
MDIGSCADILNGVLLPSSRSDLATRLVDLRQGALGRCQSLQQQQAAQAWKDLADYFNQYLAGRFPFAYSLEAADAEPGRV
RHLLKLMETRLPQVREGLAQVRSPDLPAAEDFVRRLEQAQRWLGPLFERDKSGLLGVALDIDWRSDRSLERGADQVIAWS
LYSGDQESRFPGAQDKGLTWNVGDPLKIMLRWAKNGSQRPADDPRQASLAVADLEAGWSYQGSWALLRMMRAHFSRQRPP
NVDYTEFPLVLQLPVYAPYSPENEARMFLRLSLMSLGGKTPLSIQPLPVRAPQSPFATLLPATVASTGGTP*
Nucleotide sequence: 3816 bp
>T6CP006461 NC_017548:2981490-2985305 [Pseudomonas aeruginosa M18] [TssM]
ATGAGCGGCGCGACCCTGTTCATGCTGGTTCTCCTCCTGGTGCTGCTGGCGCTGCTGCTGGGGGCGCTGGGCTGGTGGTG
GCGGACCCGGGGCGGTACGGAGATCCGCAGCTTCTACGCGGCGGTCCGGCAGATGGAGCGCGAGCAGGGCTGGCAGGGCC
GCTACGAAGCGCCCTGGCTGCTGATGCTCGGCAACGAGACCGAGGGCGAGCAGTTGTGCTCCACCTGGCGTCTGCTGCCG
GTGGCGCGCCCGGCCTGGTTCGGCCGCTGGTGGTCGGACGGCGAGGGGGCGATCCTGCTGGTGCCCGAGTCGGTGTTCCT
GCCCGACGAGGGCCTGCGCCGGCAAAGCGGCGCCTGGCTGCGCCTGTTGCGTCTGTTCCTGCGCCTGCGTGGCAGGCGCG
CGCTGGACGGAGTGGTCTGGAACATTCCGATGGCGCGGTTGCAGGATGGCGAGCAGGCGGCCAACCTCGGCCTCGCCGCG
CGCCGTCGCTACGTGGAACTGACCCAGCGCCTTGGCCTGAGCCTGCCGGTTTACGTGGTGATCACTGGCCTGGAAGACCT
GCCGGGTTTCCAGGAACTTCTCGCGGCGCTGCCCGAGGAGGCGCGCGAGCGCGCCCTCGGCTGGTCCTCGCCGTTCGCCG
CCGAAGCGGCCTGGCAGTCGCGCTGGTGCGAGCAGGCGCTGGAGGAAATCACCGCCACCCTGACCGAGTCGATCGTCGAA
CTCGGTACCTTGCGCGGCCAGGTGGACAATGAGCTGTATTGCCTGCCGCCACGCCTGGAAAGCCTCCGCGGTTCGTTGCA
GGCGCTGCTCGAACCGGTCTTCCAGGGCAACGCCAGGGGCGAGGCGCCGCGCTTTCGCGGTCTCTACCTGAGCGGCAGCG
AGGCGGCGGGCGCGGCAGCCGACGAAGTCCTGCCGGCGGTCGACGCGCCGCGTCGGCGCAGCAGCTTCGCCAGCCAACTG
TGGGCACGGCGGATCCTTGCCGAGGAAGGCCTGGCCCAGGCGGTGCCGCGCATCCTCCAGCTGCGTCAGCGCTGGCAGCG
CGGGATCGGCCTCGCCGCGTTGTGCCTGTGCCTGCTCTGGGGCGGCCTGATGACCTGGGTCTGGCGCGACGCGCTGCGCG
ACGCCGGTGAACTGTCGCAACTGCTGCATGGCGCCAGTGAGCGCTACCAGCCGCTCGACGACGACACTCGGCGCGCCGCC
CAGGTCCGGCAGAACGTCCAGGCCTGGTGGCAACTGGTGTCACAGGCGCCGCGCTGGCGCTTCACTTCGCTGGCGTTTCC
CAGTTCCTGGTTTTCCTCCCTGGATGCACGTATCGACAACGCGTATCGCCGCGTTTCCGAGCGCCTGCTGGTGCGACCGC
TGCGCAGCCTGCTGGAAGGCGAGGCGAGCGACCTGCGGGCGATCCGCAGCGATGGCCAACCCGGCCTGAAGGAGGGCGAC
GACCCCAGCCAGTGGAAGGACTACCTGGCGGCGAAGGACCTGGTCGCCCGCGCCACTACGCTGGAGCGCCACAATCGCTT
GTTCGCCCAGGCCATCGACAACCGCAGGACGCCGCTCGACGACCTTCTGCAACTGAGTAACGATGCCTTGGGCAGCAGCT
ACAACGCCGGCAGCCTGGCGCGCCTGGCCTATTACAACCGCACGCTGTTCGCCGAGCGTCCGGCCGATCTCGCCGCCCTC
GACCTGGGCCGGGTCGCCGCCACGGCGGCGGATAACTTCCATGACCTGATGGCACGCTGGCTGAACGCCTACTTCCTCAC
CGACAGCCTGGAACGCACCGGCAATGCCCTGACCCAGGAACTCGCGCAGCTCGGCCAGGAACGAGACGCCACGGCGGCCC
AGTTGCTGGGGATCGGCGAATTGATCAACCACCTGCAGCAACAGGTCAACCGCATCAACCTGATCTGGGGCAATGGCGTC
GGCCAGGATCTCGTCCCTGGCTACCAGAACCTGCTCAAGAGCGCCCAGCAAAGCAGCTTGCTGGGAAACCGCGTGGAGGA
ACTGCAGAACCTCACCGTGCAGTTGCAGCAGCAGTTTCGCAGCGAATGGATCGCGCCTCCGGAGGCTGCCGGCGACGGCC
TGCTGGTGAAACAGGGGGCGACGCTCAAGCTGGCCGATGACCTGCTCGGTCTGAAACGCGCCATCGATGACCTGAAGACC
AAGGACTTCGTCTCCGTGGCCCTGGCGGACAAGACACTGGCGGACCACTCCTTGCTGAGCATCGACGATATCGGCGTGAC
TCAGGCGTTGAGCTTCTTCAACGACTTCACCGGCTACTACGAGAGCACCCTGCCATCGCTCAACCCGCGCTATCGCTATT
CGGTCCTGCACGCCGCCGCCAGCGCGGCGACCGAGGCCATGTGGCAAAGCCTCGGGCCGCGCAGTCGTAGCCTGGCCAGT
CGCAATGCCTCGCGTTTCGACGTGCAGGTCAAGCAGGTCCAGGTGTTGCAGGCGGCGCTGCTGGAGTTGCAGGACCTGCA
GGCCGGCGCGCGCCTGCTGCTCAGCCTCAACGCGCTGGCCGTGGCCGATATCGGCCAGGCCCTGCGCGACATCGACGAGC
AGGCGGTGCTGCGCGAGCCGGTCGATTTCAGCCGCTGGTCGGGCGCGCCGAATTTCGGCCTGCAGATGTTCCGCGCCCAG
GACAGTGCCGAGCTGAAGCAAAGCCTGAACAGCCAGTTCAATGCCATGGCGGCCGTCGCCGAACGCCACGCCCCGGCGCT
GGAGTGGTTGCAGGCGCAGCAATCCAGCCTGGCCACCGCCGACTACAACGCGTTCGTGCGCTTCAGCGCGCTCAACGCCG
AACTGCAGAAATTCAAGGCCGACAACCCGGCGAGCACCGCGGCGCAGTTGGCGAAGCTGGTGGGTAACGATTTCAACCAG
ATGGACATCGGCAGTTGCGCCGACATTCTCAACGGCGTGCTCCTGCCGTCCAGCCGCAGCGACCTGGCGACGCGCCTGGT
GGACCTGCGCCAGGGCGCGCTCGGGCGCTGCCAGTCGTTGCAACAGCAGCAGGCGGCGCAGGCCTGGAAGGACCTCGCCG
ACTACTTCAACCAGTACCTCGCCGGACGCTTCCCGTTCGCCTACAGCCTGGAAGCGGCCGATGCCGAGCCGGGGCGGGTG
CGGCACCTGCTGAAACTGATGGAGACGCGCTTGCCGCAGGTGCGCGAAGGGCTGGCCCAGGTTCGCTCGCCGGACCTGCC
GGCCGCCGAGGACTTCGTCCGCCGCCTGGAGCAGGCGCAGCGCTGGCTCGGCCCGCTGTTCGAGCGCGACAAGTCGGGCC
TGCTCGGCGTCGCCCTGGACATCGACTGGCGCAGCGACCGCAGCCTGGAACGCGGCGCCGACCAGGTGATCGCCTGGAGC
CTGTACTCGGGCGACCAGGAAAGCCGTTTCCCCGGCGCGCAGGACAAGGGCCTGACCTGGAATGTCGGCGATCCGCTGAA
AATCATGCTGCGCTGGGCGAAGAACGGCTCGCAGCGTCCGGCAGACGACCCGCGCCAGGCCAGCCTGGCGGTGGCCGACC
TCGAGGCCGGCTGGAGCTACCAGGGCTCCTGGGCGCTGTTGCGCATGATGCGCGCGCACTTCTCCCGGCAGCGCCCGCCG
AACGTCGACTACACGGAGTTTCCGCTGGTCCTGCAACTGCCGGTGTATGCGCCCTACAGCCCGGAAAACGAGGCGCGGAT
GTTCCTCCGCCTGTCGTTGATGAGCCTGGGCGGCAAGACCCCGCTGTCCATCCAGCCGCTGCCGGTGCGCGCGCCGCAAT
CGCCGTTCGCGACGCTGTTGCCGGCCACCGTCGCCAGCACGGGAGGTACCCCATGA
ATGAGCGGCGCGACCCTGTTCATGCTGGTTCTCCTCCTGGTGCTGCTGGCGCTGCTGCTGGGGGCGCTGGGCTGGTGGTG
GCGGACCCGGGGCGGTACGGAGATCCGCAGCTTCTACGCGGCGGTCCGGCAGATGGAGCGCGAGCAGGGCTGGCAGGGCC
GCTACGAAGCGCCCTGGCTGCTGATGCTCGGCAACGAGACCGAGGGCGAGCAGTTGTGCTCCACCTGGCGTCTGCTGCCG
GTGGCGCGCCCGGCCTGGTTCGGCCGCTGGTGGTCGGACGGCGAGGGGGCGATCCTGCTGGTGCCCGAGTCGGTGTTCCT
GCCCGACGAGGGCCTGCGCCGGCAAAGCGGCGCCTGGCTGCGCCTGTTGCGTCTGTTCCTGCGCCTGCGTGGCAGGCGCG
CGCTGGACGGAGTGGTCTGGAACATTCCGATGGCGCGGTTGCAGGATGGCGAGCAGGCGGCCAACCTCGGCCTCGCCGCG
CGCCGTCGCTACGTGGAACTGACCCAGCGCCTTGGCCTGAGCCTGCCGGTTTACGTGGTGATCACTGGCCTGGAAGACCT
GCCGGGTTTCCAGGAACTTCTCGCGGCGCTGCCCGAGGAGGCGCGCGAGCGCGCCCTCGGCTGGTCCTCGCCGTTCGCCG
CCGAAGCGGCCTGGCAGTCGCGCTGGTGCGAGCAGGCGCTGGAGGAAATCACCGCCACCCTGACCGAGTCGATCGTCGAA
CTCGGTACCTTGCGCGGCCAGGTGGACAATGAGCTGTATTGCCTGCCGCCACGCCTGGAAAGCCTCCGCGGTTCGTTGCA
GGCGCTGCTCGAACCGGTCTTCCAGGGCAACGCCAGGGGCGAGGCGCCGCGCTTTCGCGGTCTCTACCTGAGCGGCAGCG
AGGCGGCGGGCGCGGCAGCCGACGAAGTCCTGCCGGCGGTCGACGCGCCGCGTCGGCGCAGCAGCTTCGCCAGCCAACTG
TGGGCACGGCGGATCCTTGCCGAGGAAGGCCTGGCCCAGGCGGTGCCGCGCATCCTCCAGCTGCGTCAGCGCTGGCAGCG
CGGGATCGGCCTCGCCGCGTTGTGCCTGTGCCTGCTCTGGGGCGGCCTGATGACCTGGGTCTGGCGCGACGCGCTGCGCG
ACGCCGGTGAACTGTCGCAACTGCTGCATGGCGCCAGTGAGCGCTACCAGCCGCTCGACGACGACACTCGGCGCGCCGCC
CAGGTCCGGCAGAACGTCCAGGCCTGGTGGCAACTGGTGTCACAGGCGCCGCGCTGGCGCTTCACTTCGCTGGCGTTTCC
CAGTTCCTGGTTTTCCTCCCTGGATGCACGTATCGACAACGCGTATCGCCGCGTTTCCGAGCGCCTGCTGGTGCGACCGC
TGCGCAGCCTGCTGGAAGGCGAGGCGAGCGACCTGCGGGCGATCCGCAGCGATGGCCAACCCGGCCTGAAGGAGGGCGAC
GACCCCAGCCAGTGGAAGGACTACCTGGCGGCGAAGGACCTGGTCGCCCGCGCCACTACGCTGGAGCGCCACAATCGCTT
GTTCGCCCAGGCCATCGACAACCGCAGGACGCCGCTCGACGACCTTCTGCAACTGAGTAACGATGCCTTGGGCAGCAGCT
ACAACGCCGGCAGCCTGGCGCGCCTGGCCTATTACAACCGCACGCTGTTCGCCGAGCGTCCGGCCGATCTCGCCGCCCTC
GACCTGGGCCGGGTCGCCGCCACGGCGGCGGATAACTTCCATGACCTGATGGCACGCTGGCTGAACGCCTACTTCCTCAC
CGACAGCCTGGAACGCACCGGCAATGCCCTGACCCAGGAACTCGCGCAGCTCGGCCAGGAACGAGACGCCACGGCGGCCC
AGTTGCTGGGGATCGGCGAATTGATCAACCACCTGCAGCAACAGGTCAACCGCATCAACCTGATCTGGGGCAATGGCGTC
GGCCAGGATCTCGTCCCTGGCTACCAGAACCTGCTCAAGAGCGCCCAGCAAAGCAGCTTGCTGGGAAACCGCGTGGAGGA
ACTGCAGAACCTCACCGTGCAGTTGCAGCAGCAGTTTCGCAGCGAATGGATCGCGCCTCCGGAGGCTGCCGGCGACGGCC
TGCTGGTGAAACAGGGGGCGACGCTCAAGCTGGCCGATGACCTGCTCGGTCTGAAACGCGCCATCGATGACCTGAAGACC
AAGGACTTCGTCTCCGTGGCCCTGGCGGACAAGACACTGGCGGACCACTCCTTGCTGAGCATCGACGATATCGGCGTGAC
TCAGGCGTTGAGCTTCTTCAACGACTTCACCGGCTACTACGAGAGCACCCTGCCATCGCTCAACCCGCGCTATCGCTATT
CGGTCCTGCACGCCGCCGCCAGCGCGGCGACCGAGGCCATGTGGCAAAGCCTCGGGCCGCGCAGTCGTAGCCTGGCCAGT
CGCAATGCCTCGCGTTTCGACGTGCAGGTCAAGCAGGTCCAGGTGTTGCAGGCGGCGCTGCTGGAGTTGCAGGACCTGCA
GGCCGGCGCGCGCCTGCTGCTCAGCCTCAACGCGCTGGCCGTGGCCGATATCGGCCAGGCCCTGCGCGACATCGACGAGC
AGGCGGTGCTGCGCGAGCCGGTCGATTTCAGCCGCTGGTCGGGCGCGCCGAATTTCGGCCTGCAGATGTTCCGCGCCCAG
GACAGTGCCGAGCTGAAGCAAAGCCTGAACAGCCAGTTCAATGCCATGGCGGCCGTCGCCGAACGCCACGCCCCGGCGCT
GGAGTGGTTGCAGGCGCAGCAATCCAGCCTGGCCACCGCCGACTACAACGCGTTCGTGCGCTTCAGCGCGCTCAACGCCG
AACTGCAGAAATTCAAGGCCGACAACCCGGCGAGCACCGCGGCGCAGTTGGCGAAGCTGGTGGGTAACGATTTCAACCAG
ATGGACATCGGCAGTTGCGCCGACATTCTCAACGGCGTGCTCCTGCCGTCCAGCCGCAGCGACCTGGCGACGCGCCTGGT
GGACCTGCGCCAGGGCGCGCTCGGGCGCTGCCAGTCGTTGCAACAGCAGCAGGCGGCGCAGGCCTGGAAGGACCTCGCCG
ACTACTTCAACCAGTACCTCGCCGGACGCTTCCCGTTCGCCTACAGCCTGGAAGCGGCCGATGCCGAGCCGGGGCGGGTG
CGGCACCTGCTGAAACTGATGGAGACGCGCTTGCCGCAGGTGCGCGAAGGGCTGGCCCAGGTTCGCTCGCCGGACCTGCC
GGCCGCCGAGGACTTCGTCCGCCGCCTGGAGCAGGCGCAGCGCTGGCTCGGCCCGCTGTTCGAGCGCGACAAGTCGGGCC
TGCTCGGCGTCGCCCTGGACATCGACTGGCGCAGCGACCGCAGCCTGGAACGCGGCGCCGACCAGGTGATCGCCTGGAGC
CTGTACTCGGGCGACCAGGAAAGCCGTTTCCCCGGCGCGCAGGACAAGGGCCTGACCTGGAATGTCGGCGATCCGCTGAA
AATCATGCTGCGCTGGGCGAAGAACGGCTCGCAGCGTCCGGCAGACGACCCGCGCCAGGCCAGCCTGGCGGTGGCCGACC
TCGAGGCCGGCTGGAGCTACCAGGGCTCCTGGGCGCTGTTGCGCATGATGCGCGCGCACTTCTCCCGGCAGCGCCCGCCG
AACGTCGACTACACGGAGTTTCCGCTGGTCCTGCAACTGCCGGTGTATGCGCCCTACAGCCCGGAAAACGAGGCGCGGAT
GTTCCTCCGCCTGTCGTTGATGAGCCTGGGCGGCAAGACCCCGCTGTCCATCCAGCCGCTGCCGGTGCGCGCGCCGCAAT
CGCCGTTCGCGACGCTGTTGCCGGCCACCGTCGCCAGCACGGGAGGTACCCCATGA