SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP006440
Component protein type	TssL
T6SS ID (Type)	T6SS00611 (Type i3)
Strain	Pseudomonas aeruginosa M18
Replicon	chromosome
Sequence	Protein sequence (450 a.a.); Nucleotide sequence (1350 bp)
Reference	[1] Wei X, Huang X, Tang L, et al. Global control of GacA in secondary metabolism, primary metabolism, secretion systems, and motility in the rhizobacterium Pseudomonas aeruginosa M18. J Bacteriol. 2013 Aug;195(15):3387-400. doi: 10.1128/JB.00214-13. Epub 2013 May 24. PMID: 23708134

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PAM18_RS00400
Coordinate (Strand)	93473..94822 (-)
NCBI ID	WP_003118500.1
RefSeq	NC_017548
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	9.7e-68	67..269
OmpA	PF00691.22	2.1e-14	339..437

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 450 a.a.

						>T6CP006440 NC_017548:c94822-93473 [Pseudomonas aeruginosa M18] [TssL]
MPPIDDPFGTPAGGGNGSPPDDRTMIMPRPGGRAPEPAGGPRGGDPYTPPPMASLAPLSGRGDGLNPLEQAAGPLLAMLT
RLRNTIAHPAPASLRAQLLGYLRQFEEKARQAGVAPDEVMLARYVLCTALDEAVLSTPWGSTSDWGKQSLLITLHNEAWG
GEKVFQLLEHCLQNPHQRLHLLELLYLCTSLGFEGRYRVMHDGRSQLEALRERTAAVIRSTRGEYEHELSPHWRGLSVMR
DRLSQYLPPWVGIAIGLALLLALLFGLRMKLAADAEPVFKRIHAIGEIPVQAIDRPAVQPRVIERPRLARFLADDIKAQR
VAVEDAVDRSVVTIRGDELFASASASVRDEFQPLLLRIADALRKVKGQVLVTGHSDNRPIATLRYPSNWKLSQARAQEVA
DLLGATTGDAGRFTAEGRSDTEPVASNASAEGRARNRRVEITVFAEGAQ*					

Nucleotide sequence: 1350 bp

						>T6CP006440 NC_017548:c94822-93473 [Pseudomonas aeruginosa M18] [TssL]
ATGCCTCCCATCGACGATCCCTTCGGCACCCCGGCCGGCGGCGGCAACGGAAGCCCGCCCGACGACCGCACCATGATCAT
GCCGCGTCCCGGCGGCCGCGCCCCGGAACCGGCCGGCGGCCCCCGCGGCGGCGATCCCTACACGCCGCCGCCGATGGCCA
GCCTGGCGCCGCTGAGCGGCCGCGGCGACGGCCTCAACCCGCTGGAGCAGGCCGCCGGCCCACTGCTGGCTATGCTCACC
CGCCTGCGCAACACCATCGCCCATCCGGCGCCGGCCAGCCTGCGCGCGCAACTGCTCGGCTACCTGCGCCAGTTCGAGGA
GAAGGCACGCCAGGCCGGCGTGGCGCCGGACGAGGTGATGCTGGCGCGCTACGTGCTCTGCACCGCCCTCGACGAAGCGG
TGCTCAGCACGCCGTGGGGCAGCACCAGCGACTGGGGCAAGCAGAGCCTGCTGATCACCCTGCACAACGAGGCCTGGGGC
GGCGAGAAGGTCTTCCAGTTGCTCGAACACTGCCTGCAGAACCCGCACCAGCGCCTGCACCTGCTGGAGCTGCTGTACCT
GTGCACCAGCCTCGGCTTCGAAGGCCGCTACCGGGTCATGCACGACGGCCGCAGCCAACTGGAGGCGCTGCGCGAGCGCA
CCGCGGCGGTGATCCGCAGCACCCGCGGCGAGTACGAGCACGAACTGTCGCCGCACTGGCGCGGCCTCAGCGTGATGCGC
GACCGCCTTTCGCAATACCTGCCGCCGTGGGTCGGTATCGCCATCGGCCTGGCCTTGCTGCTGGCCTTGCTGTTCGGCCT
GCGGATGAAGCTGGCCGCCGACGCCGAGCCGGTGTTCAAGCGCATCCACGCCATCGGCGAGATCCCGGTGCAGGCCATCG
ACCGCCCGGCGGTGCAGCCGCGGGTGATCGAGCGGCCACGCCTGGCGCGCTTCCTCGCCGACGACATCAAGGCCCAGCGG
GTGGCGGTGGAGGACGCCGTCGACCGCTCGGTGGTGACCATCCGCGGCGACGAGCTGTTCGCCAGCGCCAGCGCCAGCGT
GCGCGACGAGTTCCAGCCGCTGCTGCTGCGCATCGCCGACGCCCTGCGCAAGGTCAAGGGCCAGGTGCTGGTCACCGGCC
ATAGCGACAACCGGCCGATCGCCACGCTGCGCTACCCGTCCAACTGGAAGCTGTCCCAGGCCCGCGCCCAGGAGGTCGCC
GACCTGCTCGGCGCGACCACCGGCGACGCCGGCCGCTTCACCGCCGAAGGGCGCAGCGACACCGAGCCGGTGGCGAGCAA
CGCCAGCGCCGAAGGCCGCGCACGCAACCGCCGGGTGGAAATCACCGTATTCGCGGAGGGCGCGCAATGA					