SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005838
Component protein type	TssL
T6SS ID (Type)	T6SS00551 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Replicon	chromosome
Sequence	Protein sequence (435 a.a.); Nucleotide sequence (1305 bp)
Reference	[1] Liu J, Guo JT, Li YG, et al. The type VI secretion system gene cluster of Salmonella typhimurium: required for full virulence in mice. J Basic Microbiol. 2013 Jul;53(7):600-7. doi: 10.1002/jobm.201200047. Epub 2012 Sep 7. PMID: 22961625 [2] Pezoa D, Yang HJ, Blondel CJ, et al. The type VI secretion system encoded in SPI-6 plays a role in gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken. PLoS One. 2013 May 14;8(5):e63917. doi: 10.1371/journal.pone.0063917. Print 2013. PMID: 23691117

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	STM14_RS02000
Coordinate (Strand)	321796..323100 (+)
NCBI ID	WP_000132483.1
RefSeq	NC_016856
Uniprot ID	A0A3V2JQ41_SALNE, A0A5I1GE33_SALET, A0A5X7WM24_SALMO, A0A3V5VSD0_SALET, V7IK55_SALET, A0A658IDB7_SALNE, A0A3V9PSL2_SALET, A0A5I5T4T9_SALET, A0A3G3E3X5_SALET, A0A4Q8S578_SALHA, A0A5H3KKQ9_SALET, A0A5J1UY04_SALET, A0A3Z2NTI4_SALEN, A0A606SZP8_SALTM, A0A5I3TX12_SALET, A0A3V3CM97_SALET, A0A5I1NBV3_SALET, A0A418Z791_SALET, A0A5V7H2K8_SALET, A0A3Z1TWQ6_SALET, A0A5X5NIM8_SALET, A0A5H6P1B0_SALET, A0A6C8LKM8_SALET, A0A0H3N7X6_SALTS, A0A3V4QGH3_SALET, A0A3Z6QSJ2_SALET, A0A736KSX3_SALET, A0A4P9SZI5_SALET, A0A5I4KMN1_SALET, A0A5V6HGH0_SALET, A0A615FV28_SALDE, A0A3W0KDA5_SALON, A0A3V9FS23_SALSE, A0A4Y6MHI9_SALET, A0A0M0PT45_SALER, A0A0H2WRW4_SALPA, A0A603MUE0_SALET, A0A5X1VYB0_SALET, A0A2T8QQ45_SALET, A0A3T2WC79_SALET, A0A622LIX9_SALET, A0A0N1QXK4_SALSV, A0A315GLS3_SALET, A0A5C5HAN1_SALET, A0A3W0FEJ3_SALET, A0A5I9SC14_SALET, A0A5I5FWN9_SALET, A0A5I3QIL6_SALPT, A0A6C7I4S7_SALPK, A0A6C7I6S4_SALTD, A0A605YMH8_SALET, A0A3V7I8W2_SALET, A0A5W3E0G5_SALAN, A0A0F6AX84_SALT1, A0A3Y2Z2L0_SALET, A0A3U9DIV0_SALIN, A0A625ANZ1_SALMU, A0A5W9CSQ1_SALRU, A0A3V6BQF1_SALTH, A0A5I2L5L8_SALET, A0A3U8MRA4_SALET, A0A745GPA5_SALTI, H9L4F7_SALTM, E8XIU7_SALT4, A0A2T8WWM6_SALET, A0A752A2V5_SALDU, A0A3Z2FA01_SALTU, Q93IS0_SALTY
KEGG ID	sew:SeSA_A0314, sey:SL1344_0277, sek:SSPA2317, stm:STM0282, spt:SPA2482, sej:STMUK_0285, seni:CY43_01410, sev:STMMW_02871, seb:STM474_0294, seo:STM14_0330
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	3.2e-59	63..265
OmpA	PF00691.22	5.3e-16	328..426

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-242	MTDSTLTPPAADMMSFLSTTPEHKDSEYETPVHTSQRTELNVIVEDGPDSKLRLAEISAAANPLLAAARPLLCALAAMPAKLDAALVEPYRNLLVREMHLYQTLCDQANLRREHVLAVRYCLCTALDEAANNTTWGRRGVWAGKSLLVTFHGESEGGIKLFQIIGRLAASFQEHGNVLEVIYHLLGLGFEGRYSVQPDGRKQLDNIRQQLLTQLSQRRDPVMPALSPDFQGAISGRLRRMRR
Transmembrane helix	243-265	VPVWLSAGIALLAMLTLFGLYSH
Inside	266-434	RMDVQTVTVQQHIDAIGIKLPPPPVPVHKLRLKILLANEIARGLLTVDEDDQHSRVVFRGDAMFVPGQKTVSDAIRPVINKAAREIARVGGAVTVTGHTDSQPIHSAEFPSNLVLSEKRAAEVAALLTSGGVPAGRVHIVGKGDTVPVADNGSKAGRAKNRRVEILVVE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9837	-	-

Protein sequence: 435 a.a.

						>T6CP005838 NC_016856:321796-323100 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssL]
MTDSTLTPPAADMMSFLSTTPEHKDSEYETPVHTSQRTELNVIVEDGPDSKLRLAEISAAANPLLAAARPLLCALAAMPA
KLDAALVEPYRNLLVREMHLYQTLCDQANLRREHVLAVRYCLCTALDEAANNTTWGRRGVWAGKSLLVTFHGESEGGIKL
FQIIGRLAASFQEHGNVLEVIYHLLGLGFEGRYSVQPDGRKQLDNIRQQLLTQLSQRRDPVMPALSPDFQGAISGRLRRM
RRVPVWLSAGIALLAMLTLFGLYSHRMDVQTVTVQQHIDAIGIKLPPPPVPVHKLRLKILLANEIARGLLTVDEDDQHSR
VVFRGDAMFVPGQKTVSDAIRPVINKAAREIARVGGAVTVTGHTDSQPIHSAEFPSNLVLSEKRAAEVAALLTSGGVPAG
RVHIVGKGDTVPVADNGSKAGRAKNRRVEILVVE*					

Nucleotide sequence: 1305 bp

						>T6CP005838 NC_016856:321796-323100 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssL]
ATGACAGACAGTACCCTGACGCCGCCAGCGGCGGATATGATGTCCTTTTTGTCCACCACGCCGGAACATAAGGACAGTGA
ATATGAAACGCCGGTACACACCAGCCAGCGCACGGAACTCAATGTCATCGTTGAAGACGGTCCGGACAGCAAACTCCGGC
TGGCTGAAATCAGCGCGGCGGCTAACCCGTTGCTCGCCGCTGCCCGGCCTTTATTGTGCGCTCTCGCAGCCATGCCCGCT
AAACTGGATGCGGCCCTGGTAGAGCCTTACCGTAATCTGCTGGTACGCGAGATGCATCTGTACCAGACATTATGCGATCA
GGCGAACCTGCGGCGCGAGCACGTACTGGCGGTACGTTACTGCCTGTGTACGGCGCTTGATGAAGCCGCCAATAACACAA
CCTGGGGACGGCGCGGCGTCTGGGCCGGAAAAAGCCTGCTGGTAACATTTCATGGTGAAAGCGAAGGCGGGATAAAACTT
TTCCAGATCATCGGGCGTCTGGCGGCCAGCTTCCAGGAGCATGGCAACGTACTGGAGGTTATCTACCACCTGCTGGGGTT
GGGATTTGAAGGCCGCTACAGCGTGCAGCCAGACGGGCGTAAGCAACTGGACAATATTCGCCAGCAACTGCTGACACAGC
TTTCACAGCGTCGCGATCCGGTTATGCCCGCGCTCTCGCCTGACTTTCAGGGGGCGATAAGCGGACGACTGCGGCGGATG
CGCCGGGTGCCGGTCTGGCTGAGCGCCGGGATAGCCCTGTTGGCGATGCTGACGCTGTTTGGCCTTTACAGCCACCGGAT
GGATGTGCAGACCGTCACCGTACAACAGCATATTGATGCGATTGGTATAAAACTGCCGCCGCCGCCTGTGCCGGTTCATA
AGCTGCGGCTGAAAATCCTGCTGGCAAACGAAATCGCCCGTGGCCTGCTGACCGTGGACGAAGATGACCAGCACAGTAGG
GTGGTCTTCCGTGGCGACGCCATGTTTGTGCCGGGACAGAAAACGGTGAGTGACGCAATCCGGCCAGTGATTAACAAAGC
GGCGCGGGAAATCGCCCGCGTGGGCGGCGCAGTCACTGTAACGGGTCACACTGACAGCCAGCCCATTCATTCGGCTGAAT
TCCCATCCAACCTGGTACTGTCGGAAAAACGGGCGGCGGAAGTTGCGGCGTTGCTGACCTCCGGCGGCGTACCTGCCGGA
CGGGTACATATCGTCGGCAAGGGCGATACGGTGCCGGTGGCGGATAACGGCAGTAAAGCCGGGCGGGCGAAAAACCGTCG
GGTGGAAATTCTGGTAGTGGAGTGA					