SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005837
Component protein type	TssK
T6SS ID (Type)	T6SS00551 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Replicon	chromosome
Sequence	Protein sequence (448 a.a.); Nucleotide sequence (1344 bp)
Reference	[1] Liu J, Guo JT, Li YG, et al. The type VI secretion system gene cluster of Salmonella typhimurium: required for full virulence in mice. J Basic Microbiol. 2013 Jul;53(7):600-7. doi: 10.1002/jobm.201200047. Epub 2012 Sep 7. PMID: 22961625 [2] Pezoa D, Yang HJ, Blondel CJ, et al. The type VI secretion system encoded in SPI-6 plays a role in gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken. PLoS One. 2013 May 14;8(5):e63917. doi: 10.1371/journal.pone.0063917. Print 2013. PMID: 23691117

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	STM14_RS01995
Coordinate (Strand)	320456..321799 (+)
NCBI ID	WP_000118732.1
RefSeq	NC_016856
Uniprot ID	A0A5I4V4L4_SALET, A0A0R9NUA6_SALNE, A0A624B4A7_SALMO, A0A3V5VS98_SALET, V7IHI5_SALET, M7S6S3_SALDU, A0A5W7RYF9_SALET, A0A5X3P8E6_SALET, A0A658IDK4_SALNE, A0A5I5T526_SALET, A0A3G3E480_SALET, A0A4Q8S5D3_SALHA, A0A5I1ZV43_SALET, A0A5H9EWM2_SALET, A0A3T3GE84_SALET, A0A3V7LQC0_SALEN, A0A5W0ZNI9_SALTM, A0A7U1KSA9_SALET, A0A3V3U6L3_SALET, A0A5I1N9W7_SALET, A0A2T8L441_SALET, A0A659RW69_SALET, A0A5X5NGG6_SALET, A0A5H6P2C7_SALET, A0A5W0WQT1_SALET, A0A0H3N9I2_SALTS, A0A3V4QG77_SALET, A0A3Z3GFM6_SALET, A0A4Q8P4R7_SALET, A0A735B718_SALET, A0A4P9SZR9_SALET, A0A5I4KPV9_SALET, A0A3U8PAH8_SALBE, A0A3V8R6Z6_SALDE, A0A401AXJ8_SALSE, F5CQ78_SALER, A0A0H2WSR2_SALPA, A0A2T9Q8X6_SALET, A0A2T8QQ36_SALET, A0A5I6BA07_SALET, A0A3T2WDE6_SALET, A0A0H3BS82_SALNS, A0A6C7A1E0_SALDC, A0A315GMF1_SALET, A0A6C6ZME6_SALHS, A0A5I0V5H7_SALET, A0A3W0FE43_SALET, A0A5H9NBJ0_SALPT, A0A6C7I253_SALPK, A0A6C7I7K1_SALTD, A0A6Y0YT74_SALET, A0A5I2K3I8_SALBL, A0A3U5N7F4_SALET, A0A602Q5E0_SALAN, A0A0F6AX83_SALT1, A0A3Z3CAT5_SALET, A0A5W2S4J6_SALET, A0A3V0IDA0_SALIN, A0A3W0RAU9_SALMU, A0A3V6BQ74_SALTH, A0A265B9Z2_SALET, A0A5I6QKH8_SALET, Q83SZ6_SALTI, H9L4F9_SALTM, E8XIU6_SALT4, A0A5H6FK18_SALET, A0A3T3IKD8_SALDU, A0A3Z6P2Q0_SALTU, Q93IS1_SALTY
KEGG ID	stt:t2582, sek:SSPA2318, seb:STM474_0293, sey:SL1344_0276, seh:SeHA_C0319, spt:SPA2483, seni:CY43_01405, sed:SeD_A0302, sej:STMUK_0284, seo:STM14_0329, stm:STM0281, see:SNSL254_A0307, sev:STMMW_02861
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasE	PF05936.14	2e-135	20..444

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-447	MSWNDRVVWSEGQFLLPQMFQQQERYLEHVMHYRSLPLTPFFWGFSHYNIDGEALNIGKLILKEASGIFPDGTPFNAPDHTPLPPPLTILPEHLNQQICLAVPVRAPNSEETTFDNNPESLARFSVHEHDIRDANSLGRGAQLLQLSHLRLRLLPEKAVTGAWIGLPLTRITGLNPDGRIDIDHDLIPPIINYQASSLMCTWLSWINDLIRMRADSLAERLTGSDNHGHEAAEVSDYLLLQILNRFEPLLTHLAKTPLAPEVLYRYLSELAGELSTYVRPQTRRPAEYKEYKHLTPYAGLKSLVDEVQFLLNAVLIRGAQRIELKEGTYGILNAVVAPSDLADFSTLVLAIKASMPTDVLLQHFAAQTKIGPSDRLPELIRSHLPGLALQVLPVPPRQIPFQAGYIYYDIRREGALWEHIARYGGMAMHTAGEFPGLETELWGVRDK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9970	-	-

Protein sequence: 448 a.a.

						>T6CP005837 NC_016856:320456-321799 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssK]
MSWNDRVVWSEGQFLLPQMFQQQERYLEHVMHYRSLPLTPFFWGFSHYNIDGEALNIGKLILKEASGIFPDGTPFNAPDH
TPLPPPLTILPEHLNQQICLAVPVRAPNSEETTFDNNPESLARFSVHEHDIRDANSLGRGAQLLQLSHLRLRLLPEKAVT
GAWIGLPLTRITGLNPDGRIDIDHDLIPPIINYQASSLMCTWLSWINDLIRMRADSLAERLTGSDNHGHEAAEVSDYLLL
QILNRFEPLLTHLAKTPLAPEVLYRYLSELAGELSTYVRPQTRRPAEYKEYKHLTPYAGLKSLVDEVQFLLNAVLIRGAQ
RIELKEGTYGILNAVVAPSDLADFSTLVLAIKASMPTDVLLQHFAAQTKIGPSDRLPELIRSHLPGLALQVLPVPPRQIP
FQAGYIYYDIRREGALWEHIARYGGMAMHTAGEFPGLETELWGVRDK*					

Nucleotide sequence: 1344 bp

						>T6CP005837 NC_016856:320456-321799 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssK]
ATGAGCTGGAATGACCGCGTAGTCTGGAGTGAAGGACAATTTTTACTGCCGCAGATGTTTCAGCAGCAAGAGCGTTATCT
GGAACACGTCATGCATTACCGCAGCCTGCCGCTGACCCCCTTTTTCTGGGGATTCAGCCACTACAATATTGATGGCGAAG
CGCTGAACATCGGTAAACTGATACTGAAAGAGGCATCAGGGATTTTTCCTGACGGCACGCCGTTTAACGCACCGGACCAC
ACCCCGCTGCCGCCGCCACTGACCATTCTGCCGGAGCACCTGAACCAGCAGATTTGTCTGGCGGTACCGGTACGCGCGCC
GAACAGCGAAGAAACCACGTTTGACAATAACCCGGAATCATTGGCGCGTTTCTCGGTACATGAACACGACATCCGCGACG
CCAACTCGCTGGGACGTGGCGCGCAGTTATTACAGCTCAGTCATCTGCGCCTGCGGCTGCTGCCGGAAAAGGCGGTGACG
GGCGCCTGGATTGGCCTGCCGTTGACCCGCATCACCGGGTTGAACCCTGACGGGCGGATAGATATCGACCACGACCTGAT
CCCGCCCATCATTAATTATCAGGCCAGTTCACTGATGTGTACCTGGCTGTCGTGGATCAACGATCTCATCCGGATGCGGG
CCGATTCGCTGGCGGAACGGCTGACCGGCAGCGACAACCACGGCCATGAAGCAGCGGAGGTCTCCGATTACCTGCTGCTG
CAAATTCTCAATCGCTTTGAGCCGCTGCTGACTCACCTGGCGAAAACCCCGCTGGCCCCGGAGGTGCTGTACCGCTACCT
GTCCGAACTGGCCGGGGAACTCTCCACCTATGTGCGTCCACAAACGCGACGGCCCGCTGAATACAAAGAGTACAAACACC
TGACGCCCTATGCCGGGTTGAAATCGCTGGTTGATGAGGTGCAGTTCCTGCTGAACGCGGTACTGATCCGGGGCGCGCAG
CGCATCGAGCTGAAAGAGGGGACTTACGGCATCCTGAATGCGGTGGTGGCCCCTTCCGATCTTGCCGATTTCAGCACGCT
GGTACTGGCGATAAAGGCTTCAATGCCGACCGATGTGCTACTGCAACATTTTGCCGCCCAGACCAAAATCGGGCCATCCG
ATCGCCTGCCGGAACTGATCCGCTCGCATCTGCCGGGGCTGGCTTTGCAGGTTCTGCCTGTACCACCGCGCCAAATCCCG
TTTCAGGCCGGATACATCTATTACGACATCCGCCGCGAGGGAGCATTGTGGGAACACATTGCCCGTTACGGCGGGATGGC
CATGCATACCGCCGGGGAATTTCCGGGGCTGGAGACAGAACTGTGGGGAGTGCGCGATAAATGA					