SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005836
Component protein type	TssJ
T6SS ID (Type)	T6SS00551 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Replicon	chromosome
Sequence	Protein sequence (179 a.a.); Nucleotide sequence (537 bp)
Reference	[1] Liu J, Guo JT, Li YG, et al. The type VI secretion system gene cluster of Salmonella typhimurium: required for full virulence in mice. J Basic Microbiol. 2013 Jul;53(7):600-7. doi: 10.1002/jobm.201200047. Epub 2012 Sep 7. PMID: 22961625 [2] Pezoa D, Yang HJ, Blondel CJ, et al. The type VI secretion system encoded in SPI-6 plays a role in gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken. PLoS One. 2013 May 14;8(5):e63917. doi: 10.1371/journal.pone.0063917. Print 2013. PMID: 23691117

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	STM14_RS01990
Coordinate (Strand)	319916..320452 (+)
NCBI ID	WP_001007106.1
RefSeq	NC_016856
Uniprot ID	A0A625WLE6_SALEN, A0A5W0I3Q2_SALTM, A0A0H3NHR2_SALTS, A0A4P9SZB9_SALET, A0A0W3M9A9_SALER, A0A2T9Q8X2_SALET, A0A2T8QQ39_SALET, A0A315GQ59_SALET, A0A3W0FEQ1_SALET, A0A5H8FMC3_SALET, A0A6C7I818_SALTD, A0A0F6AX82_SALT1, A0A3U4VBR7_SALET, A0A3V2ZGY2_SALET, A0A3U8IVE2_SALET, A0A0D6HFG4_SALTM, E8XIU5_SALT4, A0A752A8I0_SALDU, A0A3Z6P2Q5_SALTU, Q8ZRL4_SALTY
KEGG ID	seni:CY43_01400, seo:STM14_0328, sev:STMMW_02851, stm:STM0280, seb:STM474_0292, sej:STMUK_0283, sey:SL1344_0275
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	6.3e-39	39..159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-178	VNEWRNPTRWLCAVAMPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYWSLHDNDKSVLTDDLVRRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFISGKGKVQLEAELEQSAIVITERDK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9989	pos: 25-26. LSG-CG.	VNEWRNPTRWLCAVAMPFALLLLSG

Protein sequence: 179 a.a.

						>T6CP005836 NC_016856:319916-320452 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssJ]
VNEWRNPTRWLCAVAMPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYW
SLHDNDKSVLTDDLVRRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFISGKGKV
QLEAELEQSAIVITERDK*					

Nucleotide sequence: 537 bp

						>T6CP005836 NC_016856:319916-320452 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssJ]
GTGAATGAATGGCGTAACCCCACTCGGTGGTTATGTGCGGTAGCTATGCCTTTTGCACTGCTCCTGCTTTCCGGATGCGG
CAGTAGCGATGCGTTACCTGACCTCGAATCACAGCGACTCGACCTGAGCGTGAAAGCCTCCGATAAGGTGAATCCTGACA
ATCAGAAGAAGGCCGCGCCCATTGAGATACGTGTTTATGAACTGAAAAATGACGCCGCTTTCACGACAGCTGATTACTGG
TCGCTCCATGACAACGACAAATCCGTCCTTACCGACGATTTAGTGCGTCGCGACAGCTTTATTTTGCGTCCCGGCGAAGA
GAAAAAACTGCGTCGCCCGCTGAATGCGCAGACCACGGCAATCGGCGTACTGGCCGGATACCGTAACCTGGCCAAATCGG
TCTGGCGGGTAACCTACAAAATCCCGGAAGCCCCGGAAAAAGCCTGGTACAGCAGCTTCATATCGGGGAAAGGAAAAGTG
CAGTTGGAGGCGGAACTGGAACAAAGCGCCATTGTAATTACGGAACGGGATAAATGA					