SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005829
Component protein type	TssF
T6SS ID (Type)	T6SS00551 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Replicon	chromosome
Sequence	Protein sequence (628 a.a.); Nucleotide sequence (1884 bp)
Reference	[1] Liu J, Guo JT, Li YG, et al. The type VI secretion system gene cluster of Salmonella typhimurium: required for full virulence in mice. J Basic Microbiol. 2013 Jul;53(7):600-7. doi: 10.1002/jobm.201200047. Epub 2012 Sep 7. PMID: 22961625 [2] Pezoa D, Yang HJ, Blondel CJ, et al. The type VI secretion system encoded in SPI-6 plays a role in gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken. PLoS One. 2013 May 14;8(5):e63917. doi: 10.1371/journal.pone.0063917. Print 2013. PMID: 23691117

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	STM14_RS01925
Coordinate (Strand)	307423..309306 (-)
NCBI ID	WP_000371510.1
RefSeq	NC_016856
Uniprot ID	A0A0H3ND27_SALTS, A0A4V1D9H8_SALET, A0A3W7KTL7_SALER, A0A5W0L7A0_SALET, A0A5Y1BL82_SALET, A0A6C7I8C4_SALTD, A0A0F6AX69_SALT1, A0A3U0VS82_SALET, Q93IT3_SALTM, E8XIT2_SALT4
KEGG ID	seb:STM474_0279, seo:STM14_0315, sey:SL1344_0262, sev:STMMW_02661
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	3.6e-221	1..624

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-627	MDPRLLEYYNRELSYLRETGAEFATLHPKIAARLGMQGTDIADPYVERMIEAFSFLSARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMAVVKLYPDTQEGDLAKGVTVPRDTAFVSPIPEGENTACQFRSSQDVTLWPLSIEEVRLTAAPPDMPALHRYLPPNIHVAGALRITLRTFGELTFSELAGPARLPFYLCGEERIASHLFELLHTSAVATLAGEPGHFDGELNVNLQHPVAHEGLEPGQGLLPLAWNVFHGHNLLHEFFACPERFYFFTPTGLSAGLQKVQGNVAEIVILLNRLPPDWLIHQTDAAQFSLFCTPVINLLPRTTTRIEVTHSVTEQHLVVDRTRPLDYEVFSVQEVEGLEAETTRKMIFRPLYHTRNNDEGNHGRYFSLRREPRRSSENARRYGTRTPYTGSEVFLSLVDQHEAPYPENLRHITVTAMVTNRDLPCLIPRNGRDDLTVDAAIPVAGVGLIRPPRPPQPPLAEREMAWRLIRQLSFNYLPLADLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVSCELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGHVMTWPVRTGQRGSV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9938	-	-

Protein sequence: 628 a.a.

						>T6CP005829 NC_016856:c309306-307423 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssF]
MDPRLLEYYNRELSYLRETGAEFATLHPKIAARLGMQGTDIADPYVERMIEAFSFLSARTQLKIDAEFPRFTQRLLEVVS
PNYVTPTPSMAVVKLYPDTQEGDLAKGVTVPRDTAFVSPIPEGENTACQFRSSQDVTLWPLSIEEVRLTAAPPDMPALHR
YLPPNIHVAGALRITLRTFGELTFSELAGPARLPFYLCGEERIASHLFELLHTSAVATLAGEPGHFDGELNVNLQHPVAH
EGLEPGQGLLPLAWNVFHGHNLLHEFFACPERFYFFTPTGLSAGLQKVQGNVAEIVILLNRLPPDWLIHQTDAAQFSLFC
TPVINLLPRTTTRIEVTHSVTEQHLVVDRTRPLDYEVFSVQEVEGLEAETTRKMIFRPLYHTRNNDEGNHGRYFSLRREP
RRSSENARRYGTRTPYTGSEVFLSLVDQHEAPYPENLRHITVTAMVTNRDLPCLIPRNGRDDLTVDAAIPVAGVGLIRPP
RPPQPPLAEREMAWRLIRQLSFNYLPLADLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLV
YGRGVSCELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGHVMTWPVRTGQRGSV*					

Nucleotide sequence: 1884 bp

						>T6CP005829 NC_016856:c309306-307423 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [TssF]
ATGGATCCACGGCTACTGGAGTATTACAACCGCGAACTGAGCTACCTGCGGGAGACCGGTGCTGAGTTCGCCACCCTCCA
TCCCAAAATTGCCGCCCGACTGGGGATGCAGGGAACCGATATTGCCGACCCGTATGTGGAACGCATGATCGAGGCGTTCA
GCTTTCTGAGCGCCCGCACTCAGCTAAAAATTGACGCCGAGTTTCCCCGCTTCACACAGCGGTTACTGGAAGTCGTCAGC
CCCAATTACGTCACCCCCACGCCATCGATGGCAGTGGTAAAGCTGTATCCCGATACGCAGGAAGGCGACCTGGCGAAAGG
GGTCACCGTGCCGCGCGATACCGCGTTCGTTTCCCCCATCCCGGAAGGCGAAAATACCGCCTGCCAGTTTCGCAGCAGCC
AGGACGTCACGCTGTGGCCGCTGTCCATCGAGGAGGTACGCCTGACCGCCGCGCCGCCGGATATGCCCGCCCTGCACCGC
TATCTGCCGCCTAATATTCATGTCGCCGGGGCATTGCGCATCACCCTGCGAACCTTCGGGGAACTGACTTTCAGCGAGCT
GGCCGGTCCGGCACGGCTGCCCTTTTACCTCTGCGGCGAAGAACGCATTGCGTCACACCTGTTTGAGCTGCTACATACCA
GCGCCGTCGCCACACTGGCCGGTGAACCCGGTCACTTTGACGGCGAACTGAATGTGAATCTTCAACACCCGGTGGCGCAC
GAAGGGCTGGAGCCAGGGCAGGGGCTGCTACCGCTCGCCTGGAACGTGTTTCACGGCCATAATCTGCTGCATGAATTTTT
CGCCTGTCCGGAACGGTTTTACTTCTTCACCCCGACCGGATTGTCTGCCGGGTTACAAAAGGTGCAGGGCAATGTGGCGG
AAATTGTCATACTGCTTAACCGCCTGCCGCCGGACTGGCTGATTCATCAGACGGACGCTGCACAGTTCAGCCTGTTCTGT
ACGCCGGTGATCAACCTGCTTCCTCGCACCACCACCCGGATTGAAGTCACTCACAGCGTGACGGAACAGCATCTGGTGGT
GGATCGTACCCGTCCGCTGGATTACGAGGTGTTTTCTGTTCAGGAAGTCGAAGGCCTGGAAGCGGAAACCACACGGAAGA
TGATTTTTCGTCCGCTGTATCACACCCGTAATAATGACGAAGGCAACCACGGGCGTTATTTTTCACTGCGTCGTGAACCG
CGCCGCTCGTCAGAAAACGCCCGCCGCTATGGCACCCGAACCCCCTATACCGGCTCGGAAGTCTTCCTCTCGCTGGTTGA
TCAGCATGAAGCGCCCTACCCGGAAAATCTGCGCCATATCACCGTCACGGCAATGGTGACTAACCGCGATCTGCCCTGCC
TTATTCCACGTAATGGCCGGGATGATCTGACCGTAGATGCGGCGATTCCGGTGGCGGGCGTGGGTCTTATCAGACCGCCG
CGCCCGCCGCAGCCGCCGCTGGCTGAACGCGAAATGGCCTGGCGGCTGATCCGGCAGTTGTCGTTTAATTATCTGCCGCT
GGCCGATCTGGATCACCGCACCGGCGGCCAGGCGCTGCGCGATCTGCTGAATCTGTTTATCCCGGCTCACGACAGCCCGC
AGTCGCGCCAGGTGCGCAGCCTGATTGGCTGCAAAACCACCCCGGTGACCCGCCGCCTGCCCGGCTCCGGCCTGTTGGTC
TATGGCCGGGGCGTCAGTTGCGAACTGACCGTGGACGAAGAAGGATTTTCCGGTATCAGTCCTTACCTGTTCGGTCTGGT
GCTGGAACACTATATTGCCCGCCATGTTTCCATTAATACCTTCTCGCAGATGACGCTGCACTCGATGCAACGCGGGCATG
TCATGACCTGGCCGGTCAGAACCGGGCAACGGGGGAGCGTATGA					