SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005749
Component protein type	TssI
T6SS ID (Type)	T6SS00541 (Type i1)
Strain	Pseudomonas fluorescens F113
Replicon	chromosome
Sequence	Protein sequence (688 a.a.); Nucleotide sequence (2064 bp)
Reference	[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSF113_RS59990
Coordinate (Strand)	6727385..6729448 (+)
NCBI ID	WP_014340913.1
RefSeq	NC_016830
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	1.2e-75	30..326
Phage_base_V	PF04717.14	9.4e-13	392..458

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-687	MFHTPDLRKIHLTIPDLEHDLKVLAFEGCEAISTPYSFDIELVSEQSDMDLEYLLNQQAFLAFNKQGSGVHGLIYRVVQGDTGKRLTHYKIRLVPRLTYLEHRTNQRIFQHVSVPQIITSVLKEHGILTDAHCFQLSASYSPRDYCVQYRESDLHFIERLCHEEGLHYHFQHSESGHHLVFGDDQTVFPRLEHRTAYKHGSGMVDDEPVIHRFGLRLETRTSRVTRRDYDFKKSTFPLESSCQPGVEKIQPDLEAYDYPGDFTQEKRGRLLSRRALERHRVDYRQAEGKSDQPALTTGHFLTLSGHPRQEWNDLWLLTEVHHEGKQPQVLEEITPSDTGRAEEHFNQGYRNHFVATPWDAIFRSQRVYQKPRVWGSQTAIVTGPKGEEIHCDPYGRVKVRFFWDRAGHLDDGSSCWLRVASNWAGNSYGGVTIPRVGMEVLVTFMEGDPDKPLISGCLTNSANARPYPLPAHKTRTVFRSCSSPGNNGFNELHLEDRAGQELIYLRAQRDMEQKIENDSRLEIGNERRETIRGNSITVLHAEEHRTTAADRTVQIEGNDYLRVANRHTRVDQTLAVEAGQQIQIKAGAHVVLEAGASLSLKAGGQHILINHSGIYSSSEIQLGGAPMAGATASSFTPGLTDPLAAPAPVLAPTQRALITASKVAGLDFCPICEACREGLCPMEVAAA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9936	-	-

Protein sequence: 688 a.a.

						>T6CP005749 NC_016830:6727385-6729448 [Pseudomonas fluorescens F113] [TssI]
MFHTPDLRKIHLTIPDLEHDLKVLAFEGCEAISTPYSFDIELVSEQSDMDLEYLLNQQAFLAFNKQGSGVHGLIYRVVQG
DTGKRLTHYKIRLVPRLTYLEHRTNQRIFQHVSVPQIITSVLKEHGILTDAHCFQLSASYSPRDYCVQYRESDLHFIERL
CHEEGLHYHFQHSESGHHLVFGDDQTVFPRLEHRTAYKHGSGMVDDEPVIHRFGLRLETRTSRVTRRDYDFKKSTFPLES
SCQPGVEKIQPDLEAYDYPGDFTQEKRGRLLSRRALERHRVDYRQAEGKSDQPALTTGHFLTLSGHPRQEWNDLWLLTEV
HHEGKQPQVLEEITPSDTGRAEEHFNQGYRNHFVATPWDAIFRSQRVYQKPRVWGSQTAIVTGPKGEEIHCDPYGRVKVR
FFWDRAGHLDDGSSCWLRVASNWAGNSYGGVTIPRVGMEVLVTFMEGDPDKPLISGCLTNSANARPYPLPAHKTRTVFRS
CSSPGNNGFNELHLEDRAGQELIYLRAQRDMEQKIENDSRLEIGNERRETIRGNSITVLHAEEHRTTAADRTVQIEGNDY
LRVANRHTRVDQTLAVEAGQQIQIKAGAHVVLEAGASLSLKAGGQHILINHSGIYSSSEIQLGGAPMAGATASSFTPGLT
DPLAAPAPVLAPTQRALITASKVAGLDFCPICEACREGLCPMEVAAA*					

Nucleotide sequence: 2064 bp

						>T6CP005749 NC_016830:6727385-6729448 [Pseudomonas fluorescens F113] [TssI]
ATGTTCCACACCCCTGACTTGCGCAAAATTCATCTCACAATTCCTGACCTCGAACATGACCTCAAAGTGTTGGCCTTCGA
AGGCTGCGAAGCCATTAGCACACCTTATTCGTTTGATATCGAGCTCGTCAGCGAACAAAGCGATATGGATTTGGAATACT
TGTTAAACCAGCAGGCATTTCTTGCATTCAACAAACAGGGAAGCGGTGTTCACGGGCTGATTTATCGTGTTGTACAAGGC
GACACTGGCAAACGCTTGACGCATTACAAGATAAGACTGGTTCCACGATTGACTTACTTGGAACACCGCACCAACCAGCG
TATTTTTCAACACGTCAGTGTTCCGCAGATCATTACATCGGTCCTCAAGGAGCACGGTATTCTCACTGACGCCCATTGCT
TCCAGTTGAGTGCAAGTTATTCGCCTCGTGACTACTGCGTGCAGTATCGCGAATCCGACTTGCACTTCATCGAGCGCCTG
TGTCACGAAGAAGGCCTGCATTATCACTTCCAGCACAGTGAGTCGGGCCATCACTTGGTATTTGGTGATGACCAGACTGT
ATTCCCCCGGCTTGAGCACCGCACTGCTTATAAACACGGCAGCGGCATGGTTGACGACGAGCCGGTTATCCATCGATTTG
GCCTGCGACTGGAGACCCGGACCAGCCGCGTCACGCGCCGTGATTACGATTTCAAGAAGTCGACATTCCCACTGGAGAGC
AGCTGCCAGCCCGGCGTCGAAAAGATCCAACCAGACTTGGAGGCCTATGATTATCCAGGCGACTTTACCCAGGAAAAACG
CGGCAGGTTATTGAGCCGGCGAGCCCTGGAACGTCATCGTGTCGACTACCGCCAGGCCGAAGGAAAGAGTGATCAGCCGG
CACTGACGACGGGGCACTTCCTGACATTGAGCGGGCACCCTCGTCAGGAATGGAATGATCTATGGCTCCTGACCGAAGTG
CATCACGAAGGCAAACAGCCGCAGGTACTGGAGGAAATCACCCCCAGCGACACTGGCAGGGCTGAAGAGCACTTCAATCA
GGGCTATCGCAATCACTTCGTTGCCACGCCTTGGGATGCGATCTTTCGTTCGCAACGGGTTTACCAAAAACCACGTGTAT
GGGGCAGCCAGACGGCAATCGTCACGGGGCCCAAGGGCGAAGAGATCCACTGCGACCCGTATGGCCGGGTCAAGGTCAGG
TTTTTCTGGGATCGTGCGGGGCACCTCGACGACGGCAGCAGCTGTTGGTTGCGAGTCGCCTCCAATTGGGCGGGCAACAG
CTACGGGGGCGTCACGATCCCGCGGGTGGGCATGGAGGTGTTGGTGACCTTCATGGAAGGCGATCCCGACAAGCCCCTGA
TCAGCGGATGTCTGACCAACAGCGCCAACGCTCGGCCCTACCCGCTGCCTGCGCACAAGACCCGCACCGTGTTTCGTTCT
TGCAGCTCGCCGGGCAACAACGGCTTCAACGAGCTGCACCTGGAAGACCGCGCCGGTCAGGAGCTGATCTATCTGCGGGC
CCAGCGTGACATGGAACAGAAGATCGAAAACGACAGCCGACTTGAGATTGGCAACGAGCGGCGGGAGACCATCAGGGGCA
ACAGCATCACCGTTCTGCACGCCGAGGAACATCGGACCACCGCCGCCGACCGTACGGTCCAGATCGAAGGCAATGACTAC
CTGCGCGTCGCCAACCGCCATACCCGTGTCGATCAGACACTGGCCGTCGAGGCCGGGCAGCAAATACAGATCAAGGCTGG
CGCTCATGTGGTCCTTGAAGCAGGTGCATCTCTCAGTTTGAAAGCCGGGGGCCAGCACATCCTGATCAACCACAGCGGTA
TCTACAGCAGCAGTGAAATCCAGCTCGGCGGCGCCCCCATGGCCGGCGCTACCGCTTCATCATTCACGCCTGGCTTGACC
GATCCCTTGGCGGCTCCAGCCCCCGTGCTCGCCCCCACTCAACGCGCGCTGATAACGGCCAGCAAAGTAGCGGGATTGGA
CTTTTGTCCGATTTGCGAGGCCTGCCGCGAGGGTCTCTGCCCAATGGAAGTCGCCGCCGCATGA					