SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005746
Component protein type	TssF
T6SS ID (Type)	T6SS00541 (Type i1)
Strain	Pseudomonas fluorescens F113
Replicon	chromosome
Sequence	Protein sequence (596 a.a.); Nucleotide sequence (1788 bp)
Reference	[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSF113_RS59965
Coordinate (Strand)	6721148..6722935 (-)
NCBI ID	WP_014340908.1
RefSeq	NC_016830
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	4.4e-144	3..592

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-595	MSFNHYYQSELTALRQLGRRFAERNPALAPFLGQVGRDPDVERLLEGFAFLTGRLRQKLDDQLPELSHSLMQLLWPNYMRPLPAFSILQFDPLQRSGPALRVERDTPVESKPVQQVRCRFLTCYPTEVLALDLTGLSYSVKGDGSLLSLRLEMCCDGHLGELQLSQLRLHLTGERYISQMLYLSLLRNLEGIELIPLDGSGEALRATDGSPMRFRIPADRVQPVGFAEEEALIPYPLNTFRGYRYLQEYFAFQDKFLFVDVNGLDVINAAPRSSVEQMRGLELRFDIGKSGIQRMRPTLDNIKLHCTPIVNLFKHDAQPIRLDSKQDEYLLLPADYDQEHCGVFSVESVTGWNPGGLGYQAYVPFESFEHDSSFDVPHSRPYYSVRQRPSLLHGGLDTCLGFGARHNHPHETLSIELMCTNQNLPRQLKPGDIDQPGEKSPESLSFRNIGPVTSSFAPPLNRDFLWKLISNMSLNYLSLADVNALKVILETYDFPRYYDEQTEKVSKRLLDGLKSIRHQHVDRLHRGLPVRGLRTELTIDPQGYIGEGDLFVFASVLNEFFALYASLNSYHELRVNSTQGEVYQWTPRMGQQPLL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9941	-	-

Protein sequence: 596 a.a.

						>T6CP005746 NC_016830:c6722935-6721148 [Pseudomonas fluorescens F113] [TssF]
MSFNHYYQSELTALRQLGRRFAERNPALAPFLGQVGRDPDVERLLEGFAFLTGRLRQKLDDQLPELSHSLMQLLWPNYMR
PLPAFSILQFDPLQRSGPALRVERDTPVESKPVQQVRCRFLTCYPTEVLALDLTGLSYSVKGDGSLLSLRLEMCCDGHLG
ELQLSQLRLHLTGERYISQMLYLSLLRNLEGIELIPLDGSGEALRATDGSPMRFRIPADRVQPVGFAEEEALIPYPLNTF
RGYRYLQEYFAFQDKFLFVDVNGLDVINAAPRSSVEQMRGLELRFDIGKSGIQRMRPTLDNIKLHCTPIVNLFKHDAQPI
RLDSKQDEYLLLPADYDQEHCGVFSVESVTGWNPGGLGYQAYVPFESFEHDSSFDVPHSRPYYSVRQRPSLLHGGLDTCL
GFGARHNHPHETLSIELMCTNQNLPRQLKPGDIDQPGEKSPESLSFRNIGPVTSSFAPPLNRDFLWKLISNMSLNYLSLA
DVNALKVILETYDFPRYYDEQTEKVSKRLLDGLKSIRHQHVDRLHRGLPVRGLRTELTIDPQGYIGEGDLFVFASVLNEF
FALYASLNSYHELRVNSTQGEVYQWTPRMGQQPLL*					

Nucleotide sequence: 1788 bp

						>T6CP005746 NC_016830:c6722935-6721148 [Pseudomonas fluorescens F113] [TssF]
ATGTCGTTCAATCATTACTACCAGAGCGAGCTGACGGCACTCCGCCAGTTGGGTCGACGTTTCGCCGAGCGTAACCCGGC
GTTGGCCCCCTTTCTGGGGCAGGTCGGACGGGACCCGGATGTGGAGCGGCTGCTGGAGGGGTTTGCATTCCTGACCGGGC
GGCTGCGCCAGAAGCTCGATGACCAATTGCCGGAGCTGAGTCATTCGCTGATGCAGCTGTTGTGGCCGAACTACATGCGG
CCGTTGCCGGCCTTCAGCATCTTGCAGTTCGATCCACTACAACGGTCCGGCCCCGCACTGCGGGTGGAGCGTGATACGCC
GGTCGAAAGCAAGCCTGTGCAGCAGGTGCGTTGCCGTTTCCTGACGTGTTACCCAACCGAGGTGCTGGCGCTTGATCTGA
CCGGGTTGAGCTATTCGGTGAAAGGGGATGGGTCGCTGTTGAGCCTGCGCCTGGAGATGTGTTGCGACGGTCATTTGGGC
GAACTGCAACTGAGCCAATTGCGTCTGCACTTGACCGGCGAGCGTTACATCAGCCAGATGCTCTACCTCAGCCTTTTGCG
CAATCTTGAAGGTATCGAGCTGATCCCGCTGGACGGCTCCGGCGAGGCCCTGCGGGCTACAGATGGCAGCCCTATGAGGT
TCAGGATCCCTGCCGATCGTGTCCAGCCGGTAGGGTTTGCCGAAGAGGAAGCGCTGATCCCGTATCCGCTGAATACCTTT
CGCGGCTATCGCTACTTGCAGGAGTACTTCGCTTTCCAGGACAAGTTCCTGTTCGTCGACGTCAATGGCCTGGACGTGAT
CAACGCTGCTCCGCGCAGCAGCGTCGAACAGATGCGGGGGCTGGAGCTGCGCTTCGATATTGGCAAAAGCGGTATCCAGC
GGATGCGCCCGACACTGGACAACATAAAGCTTCACTGCACGCCGATCGTCAATCTGTTCAAGCATGACGCCCAGCCAATC
CGCCTGGACAGCAAACAGGACGAATACCTGTTGCTGCCGGCCGATTATGACCAGGAGCACTGCGGTGTGTTCTCGGTCGA
AAGCGTCACCGGCTGGAACCCCGGCGGCCTGGGTTATCAGGCCTATGTGCCGTTCGAATCCTTCGAGCACGATTCAAGCT
TCGATGTGCCCCACAGCCGACCCTACTACAGCGTTCGTCAGCGCCCTTCATTGCTGCACGGTGGCCTGGACACCTGCCTA
GGCTTCGGTGCCCGGCACAATCACCCTCATGAAACCCTGTCGATCGAGTTGATGTGCACCAACCAGAACCTGCCGCGCCA
GCTCAAGCCGGGTGACATCGACCAGCCCGGCGAAAAAAGCCCCGAGTCGCTGAGTTTCCGCAACATCGGGCCGGTCACTT
CGAGCTTCGCTCCCCCGCTCAACCGCGATTTCCTCTGGAAGCTGATCAGCAACATGTCACTCAACTACCTGTCGCTGGCG
GACGTCAATGCACTCAAGGTGATTCTCGAAACCTACGATTTTCCGCGCTACTACGACGAGCAGACAGAGAAGGTCAGCAA
GCGTTTGCTGGATGGGCTCAAGTCGATCAGGCACCAGCATGTCGACCGCCTGCATCGAGGCTTGCCGGTTCGTGGCTTGC
GCACCGAATTGACCATCGACCCCCAGGGCTACATCGGCGAGGGCGACCTGTTCGTCTTCGCCTCGGTACTCAACGAATTC
TTCGCGCTTTATGCCAGCCTCAATTCGTACCACGAACTGCGGGTGAACAGTACACAAGGAGAGGTCTATCAATGGACACC
CCGCATGGGCCAGCAGCCGCTTCTATAA					