SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005740
Component protein type	TssM
T6SS ID (Type)	T6SS00541 (Type i1)
Strain	Pseudomonas fluorescens F113
Replicon	chromosome
Sequence	Protein sequence (1148 a.a.); Nucleotide sequence (3444 bp)
Reference	[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSF113_RS59920
Coordinate (Strand)	6708823..6712266 (-)
NCBI ID	WP_014340900.1
RefSeq	NC_016830
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	1.4e-67	495..768
IcmF-related_N	PF14331.8	2.4e-62	197..428
IcmF_C	PF06744.14	2.9e-19	1022..1127

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-16	MKLLFRKAGAWVRQTW
Transmembrane helix	17-39	IWTLLAVLCIALLVWFAGPLLAV
Outside	40-48	NDYKFWASP
Transmembrane helix	49-71	TARLLTVSVLMLCWGLAMVFVNG
Inside	72-423	RGGSSANFQQNAPEQARALHQARIDDERREVRSRFKQALSTLRSSSLYPGRSERWRNDLPWYLLIGPPASGKTRLLDCSGLEFPLDKLERKPIGDTSGTRYCDWYFAEHAVLVDTAGRYLTQTDSDVDSSAWTVLLELLRKRRRSRPLSGVLVTVPTEVLLLGSEDEVTTLARQIRGRLQELQQRLHVDVPVYLVLSKADGVPGFNEFFDSLTREENDQVLGASFSRDQRGSDVAVLRAEFEALLHRLNSQVIMRMHQERDPLRRSCMLDFPHQLGQLGAGLCLLVEQAFTGNVGTLRGFYLTSASPPGRTNTAPDRRSRFINHLVSRVIFPEADLTDLVQRERRRLHWGQR
Transmembrane helix	424-446	ALYLGALTVVGLCGLLWANGFSA
Outside	447-1147	NHERLDNLRTLAQRWDQQQSSQANADDWVAILDSLDIRFEATRVFPPDRAVPLHERVGLYQGDASNPAVTDAYERELQARLLPKVAQRLEAHVRNNWNDREQLLNSLRAYLMLGLQERRDVAWLKVWLANDGSLRHPGNTALQEGLNAHLERLLELPFIHALNEPLVAQAREALRRESLAVVVYRMLHEQASHLPQYRFSQHLGSQGALLVGTDRMIPGFYTRQGYERYFSVQGTALVTELLRDNWVLGEGEGLSGMDMRRLMVELEQLYFRDYADHWSEAVGQVALQTISNAGEGAEQLAGLTSAHSPILRMLVQVREHTRIQAVAEQIDDLTQAAGNGGHAVTTLAAVAGKASASLAENLPDTAQKSLRRRFEPLHRLLDPDNAPTADLVQALRAIDEVQLQLANLARASAPEQAAFELARHRMGGQRDALSHLRGASARLPRPVSAWFNVMAEDSWRLVLNDSYRYLNQRYQSELYSFYGKAINKRYPFSAHSASDVALNDFREFFKDQGIVDRFFDNYMQPFVSGSAGHYRLRSIDGQSLPMSRVYLDQMATAHTIRRSFFAENPTEPQVQFKLEPYTLDPAVSRAEFRFGDQMLEYRHGPILPVAFTWPSDAQNGRTALVLDKMVGRGVGIEKNTGAWSLFRLFDLMQSEYLTGRDVRVLKADLGGLRANYLLTSQRTPNPFDMSVLRTFRLPVQL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9824	-	-

Protein sequence: 1148 a.a.

						>T6CP005740 NC_016830:c6712266-6708823 [Pseudomonas fluorescens F113] [TssM]
MKLLFRKAGAWVRQTWIWTLLAVLCIALLVWFAGPLLAVNDYKFWASPTARLLTVSVLMLCWGLAMVFVNGRGGSSANFQ
QNAPEQARALHQARIDDERREVRSRFKQALSTLRSSSLYPGRSERWRNDLPWYLLIGPPASGKTRLLDCSGLEFPLDKLE
RKPIGDTSGTRYCDWYFAEHAVLVDTAGRYLTQTDSDVDSSAWTVLLELLRKRRRSRPLSGVLVTVPTEVLLLGSEDEVT
TLARQIRGRLQELQQRLHVDVPVYLVLSKADGVPGFNEFFDSLTREENDQVLGASFSRDQRGSDVAVLRAEFEALLHRLN
SQVIMRMHQERDPLRRSCMLDFPHQLGQLGAGLCLLVEQAFTGNVGTLRGFYLTSASPPGRTNTAPDRRSRFINHLVSRV
IFPEADLTDLVQRERRRLHWGQRALYLGALTVVGLCGLLWANGFSANHERLDNLRTLAQRWDQQQSSQANADDWVAILDS
LDIRFEATRVFPPDRAVPLHERVGLYQGDASNPAVTDAYERELQARLLPKVAQRLEAHVRNNWNDREQLLNSLRAYLMLG
LQERRDVAWLKVWLANDGSLRHPGNTALQEGLNAHLERLLELPFIHALNEPLVAQAREALRRESLAVVVYRMLHEQASHL
PQYRFSQHLGSQGALLVGTDRMIPGFYTRQGYERYFSVQGTALVTELLRDNWVLGEGEGLSGMDMRRLMVELEQLYFRDY
ADHWSEAVGQVALQTISNAGEGAEQLAGLTSAHSPILRMLVQVREHTRIQAVAEQIDDLTQAAGNGGHAVTTLAAVAGKA
SASLAENLPDTAQKSLRRRFEPLHRLLDPDNAPTADLVQALRAIDEVQLQLANLARASAPEQAAFELARHRMGGQRDALS
HLRGASARLPRPVSAWFNVMAEDSWRLVLNDSYRYLNQRYQSELYSFYGKAINKRYPFSAHSASDVALNDFREFFKDQGI
VDRFFDNYMQPFVSGSAGHYRLRSIDGQSLPMSRVYLDQMATAHTIRRSFFAENPTEPQVQFKLEPYTLDPAVSRAEFRF
GDQMLEYRHGPILPVAFTWPSDAQNGRTALVLDKMVGRGVGIEKNTGAWSLFRLFDLMQSEYLTGRDVRVLKADLGGLRA
NYLLTSQRTPNPFDMSVLRTFRLPVQL*					

Nucleotide sequence: 3444 bp

						>T6CP005740 NC_016830:c6712266-6708823 [Pseudomonas fluorescens F113] [TssM]
ATGAAATTGCTTTTCAGGAAAGCCGGTGCCTGGGTGCGCCAGACGTGGATTTGGACGCTGCTGGCGGTGCTTTGTATCGC
GTTGCTGGTGTGGTTCGCCGGGCCACTTCTGGCCGTCAATGACTACAAATTCTGGGCAAGCCCGACAGCGCGGTTGCTGA
CCGTCAGCGTGTTAATGCTGTGCTGGGGCTTGGCCATGGTTTTCGTCAATGGGCGCGGTGGTTCAAGCGCGAATTTCCAG
CAAAACGCCCCCGAGCAGGCTCGGGCCCTGCATCAGGCCAGGATTGATGATGAGCGGCGCGAAGTGCGCTCGCGCTTCAA
GCAGGCATTATCCACGCTAAGGTCCTCGAGCCTTTATCCCGGCCGCAGCGAACGCTGGCGCAACGATCTGCCGTGGTATC
TGCTGATCGGCCCGCCTGCCAGCGGCAAGACCCGTCTGCTGGACTGCTCGGGCCTGGAGTTCCCTCTCGACAAGCTTGAG
CGCAAGCCAATCGGCGACACGTCGGGTACCCGTTATTGCGATTGGTATTTTGCCGAGCACGCCGTGCTGGTCGACACTGC
CGGGCGCTACCTGACCCAGACGGACAGTGATGTCGACAGCAGTGCCTGGACCGTATTGCTCGAACTGCTGCGCAAGCGTC
GCCGCAGTCGTCCGTTGAGCGGTGTACTGGTGACGGTACCGACCGAGGTCCTGTTGCTGGGCAGCGAGGACGAAGTGACT
ACGCTGGCCCGCCAGATTCGTGGCCGCCTGCAGGAACTGCAACAGCGACTGCACGTCGATGTCCCGGTTTATCTGGTCCT
GAGCAAAGCGGACGGCGTGCCAGGTTTCAATGAGTTCTTTGATTCGTTGACTCGCGAAGAAAACGACCAGGTATTGGGTG
CAAGTTTCAGTCGGGACCAGCGTGGCAGTGATGTGGCCGTGTTACGTGCCGAGTTCGAGGCGCTGCTGCATCGCCTCAAC
AGCCAGGTGATCATGCGGATGCATCAGGAGCGCGACCCGCTGCGCCGTAGCTGCATGCTTGATTTCCCTCACCAACTTGG
ACAACTCGGTGCCGGCCTGTGTCTGCTGGTCGAGCAGGCGTTTACCGGTAACGTTGGTACGTTGCGTGGTTTCTATTTGA
CTAGCGCATCGCCGCCGGGCCGAACCAACACCGCGCCTGACCGGCGCTCGCGGTTCATCAATCACTTGGTCAGCCGGGTG
ATTTTCCCGGAAGCCGATCTGACTGATCTCGTTCAGCGCGAGCGCCGTCGTCTCCATTGGGGACAGCGGGCCTTGTACCT
CGGGGCGTTGACGGTCGTTGGCTTGTGCGGGTTGTTGTGGGCGAATGGTTTTTCGGCCAACCACGAGCGCCTGGACAACT
TGCGGACACTGGCGCAGCGCTGGGACCAGCAGCAATCATCCCAGGCTAACGCCGATGATTGGGTTGCAATACTCGACTCA
CTGGATATCCGTTTCGAGGCCACCCGGGTTTTCCCGCCCGACAGGGCGGTGCCTTTACATGAGCGCGTCGGTCTGTACCA
GGGCGATGCGAGCAACCCTGCGGTGACCGACGCCTACGAGCGTGAATTACAGGCGCGACTGTTGCCGAAGGTTGCGCAGA
GGCTGGAAGCGCATGTCCGTAACAACTGGAATGACCGCGAGCAACTGCTCAACAGCCTGCGTGCGTACTTGATGCTGGGC
CTGCAAGAGCGCCGCGATGTTGCCTGGCTCAAGGTTTGGCTGGCCAACGATGGGTCGCTTCGTCACCCCGGCAACACGGC
ACTACAGGAGGGCCTGAATGCCCACCTCGAGCGCCTGTTGGAGCTACCGTTTATCCATGCGCTCAACGAACCGCTGGTGG
CCCAGGCCCGGGAAGCCTTGCGCCGTGAGTCCCTGGCAGTCGTCGTGTACAGAATGCTGCACGAACAGGCCAGCCATCTG
CCGCAATACCGATTCAGCCAACACCTGGGCTCGCAAGGTGCGTTGTTGGTCGGTACCGATCGTATGATTCCCGGTTTCTA
TACCCGCCAGGGTTACGAACGCTACTTTTCGGTTCAAGGCACGGCCCTGGTCACCGAGTTGCTGCGGGACAACTGGGTAT
TGGGTGAAGGCGAGGGCCTCAGCGGCATGGACATGCGTCGCCTGATGGTCGAACTGGAACAGCTGTATTTTCGTGACTAT
GCCGACCACTGGAGCGAAGCGGTCGGCCAGGTCGCCCTACAAACCATCAGCAATGCAGGTGAGGGCGCCGAGCAACTCGC
GGGGCTGACCTCGGCTCACTCGCCGATTCTGCGGATGCTGGTGCAAGTGCGCGAACACACTCGAATCCAGGCTGTTGCCG
AGCAAATCGACGACCTGACGCAAGCGGCAGGGAACGGTGGACATGCCGTTACAACACTCGCCGCCGTGGCTGGGAAAGCC
TCTGCGTCCTTGGCCGAAAACCTGCCGGACACCGCGCAAAAATCCTTGCGACGACGTTTCGAGCCGCTGCATCGCCTGCT
GGACCCGGACAATGCTCCGACGGCAGATCTGGTCCAGGCCTTGCGGGCCATTGATGAGGTGCAATTGCAACTGGCGAACC
TGGCTCGCGCCAGTGCCCCGGAACAAGCGGCGTTCGAGCTGGCCAGGCACCGCATGGGCGGCCAGCGCGATGCGTTGAGT
CACCTGCGCGGTGCGTCTGCCCGCCTGCCGCGGCCGGTCAGTGCTTGGTTCAACGTGATGGCTGAGGACAGCTGGCGCTT
GGTGCTCAATGATTCTTATCGTTACCTGAACCAACGTTATCAGAGCGAGCTGTACAGCTTTTACGGCAAGGCGATCAACA
AGCGATACCCGTTCAGTGCCCACAGTGCCAGCGACGTCGCGCTCAATGACTTCCGGGAGTTCTTCAAGGACCAGGGTATC
GTCGATCGATTCTTCGACAATTACATGCAGCCCTTCGTCAGCGGCTCCGCGGGACACTACCGCCTGCGCAGCATCGACGG
TCAGAGCTTGCCGATGTCCCGGGTCTATCTCGATCAAATGGCAACCGCGCACACCATTCGCCGGAGCTTTTTCGCCGAAA
ACCCGACCGAACCGCAAGTACAGTTCAAGCTTGAGCCGTACACCCTGGACCCTGCGGTAAGCCGTGCTGAATTCCGTTTC
GGTGACCAGATGCTTGAATACCGCCACGGTCCGATCCTGCCGGTGGCGTTTACCTGGCCAAGCGACGCGCAGAATGGTCG
AACCGCCCTGGTGCTGGACAAAATGGTTGGGCGAGGCGTGGGCATCGAGAAAAATACCGGGGCGTGGTCGCTGTTTCGGT
TGTTCGACCTGATGCAGAGCGAATACCTGACCGGGCGCGATGTCCGCGTATTGAAGGCAGACCTGGGTGGCCTGCGCGCC
AACTATCTGCTGACGAGCCAACGTACGCCGAACCCTTTCGACATGAGTGTGCTGCGCACATTTCGCCTGCCGGTGCAGCT
TTGA					