Detailed information of component protein


Summary

Component ID T6CP005197
Component protein type TssL
T6SS ID (Type) T6SS00080 experimental (Type ii)
Strain Francisella tularensis subsp. tularensis SCHU S4
Replicon chromosome
Sequence Protein sequence (208 a.a.); Nucleotide sequence (624 bp)
Reference
[1] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[2] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[3] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
[4] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FTT_RS09025
Coordinate (Strand) 1783595..1784218 (+)
NCBI ID WP_003016181.1
RefSeq NC_006570
Uniprot ID A0A0B3VP77_FRATU, Q5NED1_FRATT, A0A0E2ZL94_FRATU
KEGG ID ftv:CH67_376, ftu:FTT_1706, ftc:DA46_617, ftu:FTT_1351, ftc:DA46_1624, ftv:CH67_1522, ftz:CH68_1253, ftz:CH68_113
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 6.6e-10 24..201
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-185 MKDFKEIEIILDIIKTTREIIEDNDNDNEKISYHRNNIRKSIFFLQEELLEKYSETVCKYIVFPLLAYVDEKLMLLREKSASNISWSLLQLEYYDRKDGGEYVFEITDNILSENIYPEICYQTISLILHNDFYGKYYDNIYNHSFLAYKKEIDKHIENSTIDSVNFIDIPVNSPPLSRKYSKTLK
Transmembrane helix 186-205 FLLRIGVPLGLFLLSLLILL
Outside 206-207 SW


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9957 - -


  Protein sequence: 208 a.a.    

>T6CP005197 NC_006570:1783595-1784218 [Francisella tularensis subsp. tularensis SCHU S4] [TssL]
MKDFKEIEIILDIIKTTREIIEDNDNDNEKISYHRNNIRKSIFFLQEELLEKYSETVCKYIVFPLLAYVDEKLMLLREKS
ASNISWSLLQLEYYDRKDGGEYVFEITDNILSENIYPEICYQTISLILHNDFYGKYYDNIYNHSFLAYKKEIDKHIENST
IDSVNFIDIPVNSPPLSRKYSKTLKFLLRIGVPLGLFLLSLLILLSW*

  Nucleotide sequence: 624 bp    

>T6CP005197 NC_006570:1783595-1784218 [Francisella tularensis subsp. tularensis SCHU S4] [TssL]
ATGAAAGACTTTAAAGAGATAGAAATTATTCTAGATATTATAAAAACTACAAGAGAAATTATAGAAGATAATGATAATGA
TAATGAGAAAATATCATACCATCGTAATAATATAAGAAAAAGTATTTTCTTCCTACAAGAAGAGCTCTTAGAAAAATATA
GTGAAACTGTCTGTAAATATATAGTTTTTCCGTTACTTGCATATGTTGATGAAAAATTGATGCTACTTAGGGAAAAGTCA
GCAAGCAATATTTCTTGGAGTCTACTACAATTAGAATATTATGATAGAAAAGATGGCGGTGAATATGTTTTTGAGATAAC
TGATAATATTTTATCAGAGAATATCTATCCAGAAATTTGCTACCAGACAATATCACTTATCCTACACAATGACTTCTATG
GCAAATACTATGACAATATATATAACCATAGCTTTCTTGCTTATAAGAAAGAAATTGATAAGCATATAGAAAATTCGACA
ATTGATTCGGTTAATTTTATTGATATACCCGTTAATAGCCCCCCACTGTCAAGAAAATATAGTAAGACGCTTAAATTCTT
ATTAAGAATAGGCGTTCCTTTAGGACTGTTTCTTTTAAGCTTACTAATTTTATTAAGCTGGTAA