Detailed information of component protein


Summary

Component ID T6CP005196
Component protein type IglI
T6SS ID (Type) T6SS00080 experimental (Type ii)
Strain Francisella tularensis subsp. tularensis SCHU S4
Replicon chromosome
Sequence Protein sequence (384 a.a.); Nucleotide sequence (1152 bp)
Reference
[1] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[2] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[3] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
[4] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FTT_RS09030
Coordinate (Strand) 1784240..1785391 (+)
NCBI ID WP_003016179.1
RefSeq NC_006570
Uniprot ID A0A0B6E291_FRATU, Q5NED0_FRATT, A0A0E2ZL87_FRATU
KEGG ID ftu:FTT_1707, ftu:FTT_1352, ftv:CH67_375, ftc:DA46_1625
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-383 MSQIISTLNNIDLVTQDKVNLISNMQIQAKYENAEYKSFIDQLIDNLNTTRSIDIYNLFLSLNSEICLNLDTVDNISKIISSYLSILNTNFTRISPSESKQKNQLAEKGINKFLVTMDANFAQMQNQDKLLPNNIDSIIEIVQEVIIFFEKNNLNYNSDFKIQLNKTLRKIKSSLILKDQKNDTYSDKESINQVEATITDQKTIQGSEKWKTLIEKIEVLKALVESERIFETSIVYNDIQNLLVNFDPKEYFPEIFFPLYKKIAPFIGNIHKNIDYYSSSIQWSIAQKMYNIDYKSFLDTLEKMPENNFLNSSSDITKNFFYETSSEVEKTPIEEHKIIADTDAIKEEIVIKQRTNNKKNQNDPVEKISNKIDEDYFEDLFDI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9965 - -


  Protein sequence: 384 a.a.    

>T6CP005196 NC_006570:1784240-1785391 [Francisella tularensis subsp. tularensis SCHU S4] [IglI]
MSQIISTLNNIDLVTQDKVNLISNMQIQAKYENAEYKSFIDQLIDNLNTTRSIDIYNLFLSLNSEICLNLDTVDNISKII
SSYLSILNTNFTRISPSESKQKNQLAEKGINKFLVTMDANFAQMQNQDKLLPNNIDSIIEIVQEVIIFFEKNNLNYNSDF
KIQLNKTLRKIKSSLILKDQKNDTYSDKESINQVEATITDQKTIQGSEKWKTLIEKIEVLKALVESERIFETSIVYNDIQ
NLLVNFDPKEYFPEIFFPLYKKIAPFIGNIHKNIDYYSSSIQWSIAQKMYNIDYKSFLDTLEKMPENNFLNSSSDITKNF
FYETSSEVEKTPIEEHKIIADTDAIKEEIVIKQRTNNKKNQNDPVEKISNKIDEDYFEDLFDI*

  Nucleotide sequence: 1152 bp    

>T6CP005196 NC_006570:1784240-1785391 [Francisella tularensis subsp. tularensis SCHU S4] [IglI]
ATGAGTCAGATAATATCTACACTAAATAATATTGATTTAGTAACTCAAGATAAAGTAAATCTGATTAGTAATATGCAAAT
ACAAGCAAAATATGAAAACGCTGAGTATAAGTCATTTATTGATCAATTAATAGATAATCTTAATACTACTAGAAGCATAG
ATATATATAACTTATTTCTATCTTTAAATTCAGAAATATGCCTCAATTTAGATACGGTTGATAATATCTCTAAAATTATT
AGCTCTTATTTATCTATTCTAAATACTAATTTCACAAGAATTAGTCCATCAGAATCAAAGCAAAAAAACCAGCTAGCTGA
AAAAGGAATAAACAAATTCTTAGTCACTATGGATGCAAATTTTGCGCAAATGCAGAATCAAGACAAGTTGTTACCAAATA
ACATAGATTCTATTATAGAAATTGTACAGGAAGTAATAATATTTTTTGAGAAAAATAACTTAAATTATAATTCTGATTTT
AAAATCCAACTAAATAAGACTCTCAGAAAAATAAAAAGTTCTTTGATTTTAAAAGATCAAAAAAATGATACGTATTCCGA
TAAAGAATCAATAAATCAAGTTGAAGCAACTATCACAGATCAAAAAACCATACAAGGCTCTGAGAAATGGAAAACTCTTA
TTGAAAAAATTGAAGTTTTAAAAGCTCTAGTTGAAAGTGAAAGAATATTTGAAACATCTATTGTTTATAATGATATACAA
AACTTATTAGTTAACTTCGATCCTAAAGAGTATTTTCCAGAAATTTTCTTTCCATTATACAAGAAGATAGCTCCTTTTAT
TGGCAATATCCATAAAAACATAGACTACTACTCATCAAGTATTCAGTGGAGTATAGCTCAGAAGATGTACAACATAGATT
ATAAGAGTTTTTTAGATACTCTTGAGAAAATGCCCGAAAATAATTTTTTAAATTCTTCTTCTGATATAACTAAAAATTTC
TTTTATGAAACATCCTCAGAGGTGGAAAAAACTCCTATCGAAGAACATAAAATAATTGCAGATACAGATGCTATCAAAGA
AGAAATAGTAATAAAACAGCGAACCAATAATAAAAAAAACCAGAATGACCCCGTAGAAAAAATTTCAAATAAAATAGATG
AAGACTATTTTGAAGATCTTTTTGACATATAA