Detailed information of component protein


Summary

Component ID T6CP005193
Component protein type PdpE
T6SS ID (Type) T6SS00080 experimental (Type ii)
Strain Francisella tularensis subsp. tularensis SCHU S4
Replicon chromosome
Sequence Protein sequence (192 a.a.); Nucleotide sequence (576 bp)
Reference
[1] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[2] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[3] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
[4] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FTT_RS09045
Coordinate (Strand) 1790174..1790749 (+)
NCBI ID WP_003022145.1
RefSeq NC_006570
Uniprot ID Q5NEC7_FRATT
KEGG ID ftu:FTT_1355, ftu:FTT_1710
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-191 MSKKIFKLLSISIIFSIYQQTFSNTIIASTFELKNSSKKNATIFLNLNEFCPTNIAACKDENDNNCIYITELVREPLSRIYYYLAVPITSKSVKDLTILYKHDKEESPPEAINICLSLFKNNKHETYYSQVIYNWFVSSELYLSFSEGSIFINKDINNSNNYDKGNFLSVNLERIADGWAPPHYEKKITII


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.8692 pos: 23-24. TFS-NT. MSKKIFKLLSISIIFSIYQQTFS


  Protein sequence: 192 a.a.    

>T6CP005193 NC_006570:1790174-1790749 [Francisella tularensis subsp. tularensis SCHU S4] [PdpE]
MSKKIFKLLSISIIFSIYQQTFSNTIIASTFELKNSSKKNATIFLNLNEFCPTNIAACKDENDNNCIYITELVREPLSRI
YYYLAVPITSKSVKDLTILYKHDKEESPPEAINICLSLFKNNKHETYYSQVIYNWFVSSELYLSFSEGSIFINKDINNSN
NYDKGNFLSVNLERIADGWAPPHYEKKITII*

  Nucleotide sequence: 576 bp    

>T6CP005193 NC_006570:1790174-1790749 [Francisella tularensis subsp. tularensis SCHU S4] [PdpE]
ATGAGTAAAAAAATATTTAAATTATTATCAATTTCAATAATATTTAGTATATATCAACAAACATTTAGTAATACAATAAT
AGCTAGTACATTCGAACTAAAAAATAGTTCTAAAAAAAATGCTACTATCTTTCTAAATTTGAACGAGTTCTGTCCGACTA
ATATAGCAGCATGCAAAGATGAGAATGACAATAATTGTATTTATATAACTGAATTAGTTAGAGAACCTCTTAGTCGTATT
TACTATTACTTAGCCGTGCCAATTACCAGCAAATCAGTTAAAGACTTAACAATATTATATAAACATGATAAAGAAGAATC
TCCACCAGAAGCAATAAATATATGTTTGAGTTTATTTAAAAATAATAAGCATGAAACATACTATTCTCAAGTTATATATA
ATTGGTTTGTATCTTCAGAATTATATTTATCATTTAGTGAAGGATCTATTTTTATAAATAAAGATATTAATAATTCTAAT
AATTATGATAAAGGTAATTTTCTAAGCGTAAATCTCGAACGTATAGCTGATGGCTGGGCCCCTCCTCATTATGAAAAGAA
AATTACTATAATATAA