Detailed information of component protein


Summary

Component ID T6CP005182
Component protein type PdpB
T6SS ID (Type) T6SS00080 experimental (Type ii)
Strain Francisella tularensis subsp. tularensis SCHU S4
Replicon chromosome
Sequence Protein sequence (1094 a.a.); Nucleotide sequence (3282 bp)
Reference
[1] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[2] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[3] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
[4] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FTT_RS08995
Coordinate (Strand) 1775727..1779008 (+)
NCBI ID WP_003022126.1
RefSeq NC_006570
Uniprot ID Q5NED7_FRATT
KEGG ID ftu:FTT_1345, ftu:FTT_1700
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MNFIKN
Transmembrane helix 7-29 HQILIISLTIAIIVLSVLGIFMI
Outside 30-1093 KKWLKTSRNPNKKSASKTIKNAKSLIKKNKVKNNLSELYIFYGDYLAAKDYITKIKPQAQIIDNNELPLIAIDKNNTSLVFANNEFSYLYKLKKKLNLQFYKLNFCFDISNDYYHENIKISEIYKELLKLRLKNFIISFYTLSEHTRSYNNAIDKKSIFKLTSDADTNHKAEEEILSKILSSKDDVNNKYNNVKILNQIRRWLKNIHPQLKSINKETFFNFGLNTPDNIIAKKDTIFFSSSPKSIIINLISLLFSFIFIVNIFQEINIKEKFSIQELNQLHESDSNKIIEETRKKIDEAILLDILYPKSIKYHFIYEQYADTLLKNVILPKYNDTYDLSMITSFLIFFEYLDNKIINQHTNRILKIISSITNLSEKQLDIVIKYTTPSIRTEMIKSAINRANKLYTNRLIVVGEDSESYLSTKGFNAEYLGISNTKRAKVINNIYIQYLYKCATDNAILDLNDNYIIPIEVNNIFKMYQEQFDTSSKKLCNSSYINSITKSVSLILNQEENKRKFESFSDMLDHIYYLVKSLEKNAKDVADDGKKQTSSITVSLINYVINSVVNPTYNKNELPLISPVSKNLYMEFAPNFYSKRILISPIYSKEYIKDNIDPTNKKFNRLINNLKDNFNIEPDFMIAIYKSSINNYIEKYIGSYNKMIDSLNNDSEFNKNISNKGALKLYLLAMSSKDSSFNSLIEFYSTNTDLITNESETNSSKKINKLQHFYGEITKLNKQNNTNDQNDKSLWAPIDNYFKENDKYLESKSYADYKNIFKQLNNLIIEQGYSSTYKNIKQGYEPLQKVYSELSKINNHQGNNNLYKLLKKHLDIAINTIKTIAIQDAITKLDNTVNIEYEYINNQFPFNKDSNSAATNEAITKDFDNNGYIYSGFIEQLSPLLSYNKTDDRWESKDFSKPEEIDYLNKFNKVYLLNKLLWDDKRNPKAIKFDITPIPNKDNDYTFFSIILDKQNYVNSLNIKYSDSIEVLYNWNSLESTTITIKFEDGSNEQISYQGKWSILKAIKDANCDQNNICTWVIKHKGKKYPLSFKIESKFLQVLGWQKQGEVNVQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9958 - -


  Protein sequence: 1094 a.a.    

>T6CP005182 NC_006570:1775727-1779008 [Francisella tularensis subsp. tularensis SCHU S4] [PdpB]
MNFIKNHQILIISLTIAIIVLSVLGIFMIKKWLKTSRNPNKKSASKTIKNAKSLIKKNKVKNNLSELYIFYGDYLAAKDY
ITKIKPQAQIIDNNELPLIAIDKNNTSLVFANNEFSYLYKLKKKLNLQFYKLNFCFDISNDYYHENIKISEIYKELLKLR
LKNFIISFYTLSEHTRSYNNAIDKKSIFKLTSDADTNHKAEEEILSKILSSKDDVNNKYNNVKILNQIRRWLKNIHPQLK
SINKETFFNFGLNTPDNIIAKKDTIFFSSSPKSIIINLISLLFSFIFIVNIFQEINIKEKFSIQELNQLHESDSNKIIEE
TRKKIDEAILLDILYPKSIKYHFIYEQYADTLLKNVILPKYNDTYDLSMITSFLIFFEYLDNKIINQHTNRILKIISSIT
NLSEKQLDIVIKYTTPSIRTEMIKSAINRANKLYTNRLIVVGEDSESYLSTKGFNAEYLGISNTKRAKVINNIYIQYLYK
CATDNAILDLNDNYIIPIEVNNIFKMYQEQFDTSSKKLCNSSYINSITKSVSLILNQEENKRKFESFSDMLDHIYYLVKS
LEKNAKDVADDGKKQTSSITVSLINYVINSVVNPTYNKNELPLISPVSKNLYMEFAPNFYSKRILISPIYSKEYIKDNID
PTNKKFNRLINNLKDNFNIEPDFMIAIYKSSINNYIEKYIGSYNKMIDSLNNDSEFNKNISNKGALKLYLLAMSSKDSSF
NSLIEFYSTNTDLITNESETNSSKKINKLQHFYGEITKLNKQNNTNDQNDKSLWAPIDNYFKENDKYLESKSYADYKNIF
KQLNNLIIEQGYSSTYKNIKQGYEPLQKVYSELSKINNHQGNNNLYKLLKKHLDIAINTIKTIAIQDAITKLDNTVNIEY
EYINNQFPFNKDSNSAATNEAITKDFDNNGYIYSGFIEQLSPLLSYNKTDDRWESKDFSKPEEIDYLNKFNKVYLLNKLL
WDDKRNPKAIKFDITPIPNKDNDYTFFSIILDKQNYVNSLNIKYSDSIEVLYNWNSLESTTITIKFEDGSNEQISYQGKW
SILKAIKDANCDQNNICTWVIKHKGKKYPLSFKIESKFLQVLGWQKQGEVNVQ*

  Nucleotide sequence: 3282 bp    

>T6CP005182 NC_006570:1775727-1779008 [Francisella tularensis subsp. tularensis SCHU S4] [PdpB]
ATGAATTTTATTAAAAATCATCAAATATTAATAATTTCACTAACTATAGCAATAATTGTTTTATCAGTACTTGGTATTTT
TATGATTAAAAAATGGCTTAAAACATCAAGAAATCCTAACAAAAAATCTGCCTCAAAGACCATAAAAAATGCAAAATCAT
TGATCAAAAAGAATAAAGTAAAAAACAACTTAAGTGAATTGTATATTTTCTATGGGGACTATCTGGCAGCTAAAGATTAT
ATAACAAAGATAAAACCTCAAGCTCAAATCATTGATAATAATGAACTCCCTTTAATAGCTATTGATAAAAACAATACATC
TCTCGTATTTGCAAATAATGAATTTTCGTACCTATATAAGTTAAAAAAGAAACTAAATCTACAATTTTATAAGCTTAACT
TTTGCTTCGATATATCCAATGATTACTACCATGAAAATATCAAAATTAGTGAAATCTATAAAGAACTATTAAAATTAAGA
TTAAAAAACTTCATAATATCTTTTTACACCTTATCTGAGCATACTAGAAGCTATAATAATGCAATTGATAAAAAATCAAT
ATTTAAATTAACTTCTGATGCGGATACAAATCATAAAGCTGAAGAGGAAATATTATCAAAAATTCTTAGCTCTAAAGATG
ATGTTAATAATAAATACAATAATGTTAAGATATTAAATCAAATAAGAAGGTGGCTAAAAAACATCCACCCTCAATTAAAA
TCCATAAATAAAGAAACATTCTTTAATTTTGGGCTAAACACACCAGATAATATCATAGCCAAGAAAGATACAATATTTTT
TAGCAGCTCTCCAAAAAGTATTATCATAAACTTAATATCTTTGTTATTTAGTTTTATATTTATCGTTAATATTTTTCAAG
AAATTAACATTAAAGAAAAATTCTCTATACAAGAGCTTAATCAACTACATGAAAGCGACTCTAATAAAATAATAGAAGAA
ACTAGAAAAAAAATTGATGAGGCTATACTTCTAGATATCCTATATCCGAAATCGATAAAATATCATTTCATATACGAACA
ATATGCTGATACTCTCCTAAAGAATGTAATCTTACCAAAATATAATGATACATATGACTTATCAATGATTACATCATTTT
TAATATTTTTTGAGTATTTGGATAACAAAATTATTAACCAACATACGAATAGAATACTTAAAATAATAAGCAGTATCACC
AACCTATCTGAAAAGCAGCTAGATATCGTGATCAAGTATACGACCCCAAGTATAAGAACTGAAATGATTAAGTCAGCCAT
CAACAGAGCAAATAAACTTTATACAAATCGTTTAATAGTTGTAGGTGAAGACTCCGAAAGTTACTTAAGTACTAAAGGAT
TTAATGCTGAATACTTAGGGATTTCAAATACTAAAAGAGCGAAGGTCATAAATAATATTTATATACAATACCTATATAAA
TGTGCTACAGATAATGCAATTTTAGATTTAAACGATAACTACATAATACCAATAGAAGTAAATAATATCTTCAAGATGTA
TCAAGAGCAATTTGACACATCCTCTAAAAAGCTATGTAACTCATCATATATTAACTCTATTACAAAGAGCGTTTCTTTGA
TACTTAATCAAGAGGAAAATAAAAGAAAATTTGAAAGCTTCAGTGATATGCTAGACCATATCTATTATTTAGTAAAATCA
TTAGAAAAAAATGCTAAAGATGTCGCAGATGATGGTAAAAAACAAACTAGCTCGATAACAGTATCGCTTATAAATTACGT
TATAAATAGTGTTGTCAACCCTACTTATAATAAAAATGAGCTCCCACTAATAAGTCCAGTAAGTAAAAACCTATATATGG
AGTTTGCTCCTAATTTCTATAGTAAAAGAATACTTATCTCTCCAATATACTCAAAAGAATATATTAAAGATAATATAGAT
CCTACAAATAAAAAGTTTAATAGACTCATCAATAATCTAAAGGATAACTTCAATATAGAACCCGACTTTATGATTGCTAT
CTATAAAAGTTCTATAAATAACTACATTGAGAAATATATAGGATCCTATAATAAGATGATTGATAGCCTTAATAATGACT
CTGAATTTAACAAAAATATATCTAACAAGGGTGCTCTTAAATTATATTTGCTAGCAATGTCATCAAAGGATTCTTCCTTT
AATAGCCTAATAGAGTTTTATAGCACTAATACAGACCTAATAACTAATGAATCCGAAACCAATTCATCTAAAAAGATAAA
TAAGCTTCAACATTTTTATGGAGAAATAACTAAATTAAACAAACAAAACAATACTAATGATCAAAATGATAAGTCATTGT
GGGCGCCTATAGATAACTACTTTAAAGAAAACGATAAATATCTTGAGTCTAAGAGTTATGCCGACTATAAAAACATATTT
AAACAGTTAAATAATCTAATAATAGAACAAGGCTATTCAAGTACATATAAAAATATCAAACAAGGATATGAACCACTACA
AAAAGTTTACTCTGAACTTTCTAAGATAAATAATCATCAAGGTAATAATAACTTATATAAACTGTTGAAGAAACATCTAG
ATATTGCCATTAATACTATAAAAACTATCGCCATACAAGATGCTATAACTAAACTTGATAATACTGTCAATATTGAGTAC
GAATACATAAATAATCAATTCCCTTTTAATAAGGATAGTAACTCGGCTGCAACAAATGAAGCTATAACCAAAGACTTTGA
TAACAATGGTTATATATACTCTGGATTTATAGAGCAACTATCTCCACTACTTTCTTATAATAAAACTGATGATAGATGGG
AAAGTAAAGATTTTTCAAAACCTGAGGAAATAGACTATCTAAATAAATTCAATAAGGTATATCTTTTAAATAAGCTACTA
TGGGATGATAAAAGAAATCCAAAAGCTATAAAATTTGATATAACTCCTATACCAAACAAAGATAATGATTATACATTTTT
TAGCATAATCCTCGATAAGCAAAACTATGTAAACTCATTAAACATAAAATATTCAGATAGTATTGAAGTTTTGTACAACT
GGAATTCACTTGAGTCAACAACTATTACCATAAAATTTGAGGATGGTAGTAATGAGCAAATAAGCTATCAAGGAAAATGG
TCTATATTAAAAGCAATTAAAGACGCAAATTGTGATCAAAACAATATTTGTACCTGGGTAATCAAGCACAAAGGTAAAAA
ATATCCTTTAAGCTTCAAAATAGAATCAAAATTCTTACAAGTATTAGGATGGCAAAAACAAGGAGAAGTCAATGTACAAT
AA