Detailed information of component protein


Summary

Component ID T6CP005168
Component protein type IglF
T6SS ID (Type) T6SS00081 experimental (Type ii)
Strain Francisella tularensis subsp. tularensis SCHU S4
Replicon chromosome
Sequence Protein sequence (577 a.a.); Nucleotide sequence (1731 bp)
Reference
[1] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[2] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[3] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
[4] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) FTT_RS07070
Coordinate (Strand) 1386552..1388282 (+)
NCBI ID WP_003022130.1
RefSeq NC_006570
Uniprot ID A0A3N3ZYX1_FRATU, A0A6N3SBQ7_FRATT, Q5NED4_FRATT
KEGG ID ftu:FTT_1348, ftu:FTT_1703
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-576 MNNNIDKWFENLFSNIHLYYKQEQSYKISKLNECITNVIKFINIKDYRKADIYNLTYVIEDVRYSTNLILSDSAIKFNDLILKKLDNLLDCTDINYFTSLMKNLKVLLEKYKLVIEKDISNRIELIKTKQIDKLESIFLDYINNHSISAYDDSLVKLYVKTIQNPNSIEAIDEYKSYFDTLKIFIKDHKNIVSFIPFRKNPILSLLKLAYLIRNGLYKTDRLLASDIILLRALYSINKDTYKLSLINEKTDTNLSIVSLTSLQAKPSENLKKTIDFIDLQIFAISQYFDDFPLQDIFFQKKSQIDIFKSESLEQLIFSLKSISNIMFDEETLYKKIHIKNQLYKNLFLNNHNSLIEDIIEKSPANLLTKLANKYFQILLDIATIINIQLVSNNLKLIYPFLEFEKYFNQVTLEVSKKSQFNQEKLEKNILNIIRIYPLLNQNYQLLKDMEQKIIDDKNSIESNDIYKLSVFVNSKSFSTYKEIRTLTSNDHKDINIHKSLAKLNKNICNAKHKNAAETAKELTIVLLSKSYYMNPTLIGVYNLPPISNSFFLVLKEITNNPIIDSIKSKQEAYWKI



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9951 - -



  Protein sequence: 577 a.a.    

>T6CP005168 NC_006570:1386552-1388282 [Francisella tularensis subsp. tularensis SCHU S4] [IglF]
MNNNIDKWFENLFSNIHLYYKQEQSYKISKLNECITNVIKFINIKDYRKADIYNLTYVIEDVRYSTNLILSDSAIKFNDL
ILKKLDNLLDCTDINYFTSLMKNLKVLLEKYKLVIEKDISNRIELIKTKQIDKLESIFLDYINNHSISAYDDSLVKLYVK
TIQNPNSIEAIDEYKSYFDTLKIFIKDHKNIVSFIPFRKNPILSLLKLAYLIRNGLYKTDRLLASDIILLRALYSINKDT
YKLSLINEKTDTNLSIVSLTSLQAKPSENLKKTIDFIDLQIFAISQYFDDFPLQDIFFQKKSQIDIFKSESLEQLIFSLK
SISNIMFDEETLYKKIHIKNQLYKNLFLNNHNSLIEDIIEKSPANLLTKLANKYFQILLDIATIINIQLVSNNLKLIYPF
LEFEKYFNQVTLEVSKKSQFNQEKLEKNILNIIRIYPLLNQNYQLLKDMEQKIIDDKNSIESNDIYKLSVFVNSKSFSTY
KEIRTLTSNDHKDINIHKSLAKLNKNICNAKHKNAAETAKELTIVLLSKSYYMNPTLIGVYNLPPISNSFFLVLKEITNN
PIIDSIKSKQEAYWKI*

  Nucleotide sequence: 1731 bp    

>T6CP005168 NC_006570:1386552-1388282 [Francisella tularensis subsp. tularensis SCHU S4] [IglF]
ATGAATAATAATATTGATAAATGGTTTGAGAATTTATTTAGCAATATCCATTTATATTATAAACAAGAGCAATCTTATAA
AATAAGTAAGCTTAATGAGTGCATCACTAACGTAATCAAATTTATAAATATAAAAGATTATAGAAAAGCTGACATCTATA
ATCTGACCTATGTTATTGAAGATGTAAGATACTCAACGAATCTAATTTTATCTGACTCTGCAATTAAATTTAATGATTTA
ATATTAAAAAAATTAGATAACCTCTTAGATTGTACTGACATTAACTATTTTACTAGCCTTATGAAGAATTTAAAGGTTCT
TTTAGAAAAGTATAAACTAGTAATAGAGAAAGATATATCGAATAGAATAGAACTTATAAAAACTAAGCAGATCGATAAAC
TAGAATCTATATTTTTAGACTATATAAACAACCATAGTATCAGCGCTTATGATGATAGTCTTGTAAAATTATATGTAAAA
ACTATACAAAATCCCAATTCCATTGAGGCTATAGATGAGTATAAATCATATTTCGATACGCTCAAAATATTTATAAAAGA
TCATAAAAATATAGTTAGTTTTATTCCCTTTAGAAAAAATCCTATTTTAAGCTTATTGAAATTAGCTTATTTAATTAGAA
ATGGTTTATATAAAACAGATCGTCTTTTAGCTAGCGATATTATTCTTTTAAGAGCTTTATATTCTATAAATAAAGATACA
TATAAATTAAGTCTTATAAATGAAAAAACAGATACTAACCTATCAATAGTTTCGCTGACGTCACTACAAGCTAAGCCCTC
AGAAAACTTAAAAAAAACTATTGATTTTATAGATTTACAGATATTTGCTATATCTCAGTATTTTGATGATTTCCCTTTAC
AGGATATTTTTTTTCAAAAAAAATCACAAATAGACATTTTCAAGTCAGAATCACTAGAACAACTTATTTTCAGTTTAAAA
AGTATTTCAAATATAATGTTTGATGAAGAAACACTATATAAAAAAATCCATATAAAAAATCAACTCTACAAAAACCTTTT
TTTAAATAATCACAATAGTTTGATAGAAGATATTATAGAAAAATCTCCAGCAAACTTATTAACAAAGCTAGCTAATAAAT
ACTTCCAAATACTGCTAGACATAGCAACAATAATAAATATTCAATTAGTAAGTAATAATTTAAAGCTTATTTATCCTTTC
TTAGAGTTTGAAAAATATTTTAATCAAGTAACTTTAGAAGTATCAAAAAAATCACAGTTTAATCAAGAAAAGCTAGAAAA
GAATATTCTAAATATTATCAGGATATATCCTTTACTAAATCAAAACTACCAATTATTAAAGGATATGGAACAAAAAATTA
TTGATGATAAAAATAGTATAGAATCAAACGATATATATAAGCTTAGTGTTTTTGTAAATAGCAAAAGTTTTTCTACCTAT
AAAGAAATCAGAACCTTAACCTCAAATGACCATAAAGATATTAACATCCACAAATCTTTAGCAAAACTGAACAAAAATAT
TTGTAATGCCAAACATAAGAATGCCGCTGAAACAGCTAAAGAGCTAACGATAGTTCTTTTAAGTAAATCTTACTATATGA
ATCCAACTTTGATAGGAGTATATAATCTACCGCCAATTTCAAATAGTTTTTTTTTGGTGCTTAAAGAGATTACAAATAAT
CCGATCATTGATAGTATAAAAAGCAAGCAAGAAGCTTATTGGAAAATTTAA