Detailed information of component protein


Summary

Component ID T6CP005163
Component protein type TssI
T6SS ID (Type) T6SS00463 experimental (Type i2)
Strain Escherichia coli 042
Replicon chromosome
Sequence Protein sequence (842 a.a.); Nucleotide sequence (2526 bp)
Reference
[1] Durand E, Zoued A, Spinelli S, et al. Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 2012 Apr 20;287(17):14157-68. doi: 10.1074/jbc.M111.338731. Epub 2012 Feb 27. PMID: 22371492
[2] Felisberto-Rodrigues C, Durand E, Aschtgen MS, et al. Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 2011 Nov;7(11):e1002386. doi: 10.1371/journal.ppat.1002386. Epub 2011 Nov 10. PMID: 22102820
[3] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558
[4] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962
[5] Zoued A, Durand E, Bebeacua C, et al. TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system. J Biol Chem. 2013 Sep 20;288(38):27031-27041. doi: 10.1074/jbc.M113.499772. Epub 2013 Aug 6. PMID: 23921384
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) EC042_RS24215
Coordinate (Strand) 4863083..4865608 (+)
NCBI ID WP_001054503.1
RefSeq NC_017626
Uniprot ID D3GUW4_ECO44, A0A3W2RZ19_ECOLX, B7LG62_ECO55
KEGG ID eck:EC55989_3331, elo:EC042_4533
PDB ID 6SJL, 6SK0, 6SKI
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 2.9e-59 39..360
DUF2345 PF10106.11 2.3e-46 616..763
T6SS_Vgr PF13296.8 1.1e-34 496..598
Phage_base_V PF04717.14 1.8e-07 406..477
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-841 MNLTDSLQNVLSGVGTLNRYRLDIPSCPSSLDVEDFSGTEGISKIYRYDIIFTSTDRYPDAAWFLRKSATLIMGTGLLESLTEQKKVHGVITDFRRISGSEDQAQYRITLKPFISLLDKQFRTHRFFVNKSVPEVVEQILTEHGLKGWEYEFSLKRTYPKREQINQYQESDLRFIQRLLAEVGIFYFFTLHPDAQTEVIHFGDVQAALIFDKTLPVNSPSGMSDSGTDSVWALNVEHRVVESRVITNDYNHREAQNILMSVPADMTRGEGYDTSYGEVYHYRPRHTERGDKIDPAPETANFWARLDHERFLAEQTRITGKSTDASLLPAQVLTITDSTPPVLPAPLQEPVLLTQLLFTGSRKSALQVMLSAVPYSEIVCWRPPLLTRPKITGTMTARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFPLIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKRGEEHIKLSTEYGGKTQLNLGHNVDASRELRGEGAELRTDDWISIRGGKGIFISADMQPQAQGKMLDMDEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNASLTDLTAPGMLLHAPDGIGMVSARALRIASGSESVGIMSGDNTDITAGQSFTVVAEGAVSLLSRNQGMQLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGNIELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTTAEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9745 - -


  Protein sequence: 842 a.a.    

>T6CP005163 NC_017626:4863083-4865608 [Escherichia coli 042] [TssI]
MNLTDSLQNVLSGVGTLNRYRLDIPSCPSSLDVEDFSGTEGISKIYRYDIIFTSTDRYPDAAWFLRKSATLIMGTGLLES
LTEQKKVHGVITDFRRISGSEDQAQYRITLKPFISLLDKQFRTHRFFVNKSVPEVVEQILTEHGLKGWEYEFSLKRTYPK
REQINQYQESDLRFIQRLLAEVGIFYFFTLHPDAQTEVIHFGDVQAALIFDKTLPVNSPSGMSDSGTDSVWALNVEHRVV
ESRVITNDYNHREAQNILMSVPADMTRGEGYDTSYGEVYHYRPRHTERGDKIDPAPETANFWARLDHERFLAEQTRITGK
STDASLLPAQVLTITDSTPPVLPAPLQEPVLLTQLLFTGSRKSALQVMLSAVPYSEIVCWRPPLLTRPKITGTMTARVTS
AKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFPLIQGTEVAIAFEEGDPDRPYIAHALHDS
RHVDHVTDKNGTRNVIRTPANNKLRMEDKRGEEHIKLSTEYGGKTQLNLGHNVDASRELRGEGAELRTDDWISIRGGKGI
FISADMQPQAQGKMLDMDEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNASLTDLTAPGMLLHAPDGIGMVSAR
ALRIASGSESVGIMSGDNTDITAGQSFTVVAEGAVSLLSRNQGMQLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVT
VSANQELILACGGAYIKLSGGNIELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE*

  Nucleotide sequence: 2526 bp    

>T6CP005163 NC_017626:4863083-4865608 [Escherichia coli 042] [TssI]
ATGAATCTCACTGACTCCCTGCAAAATGTTTTATCCGGGGTGGGAACCCTGAACCGCTACAGACTGGATATACCTTCCTG
CCCGTCATCGCTGGATGTTGAAGATTTCAGCGGAACTGAAGGCATAAGTAAGATTTACCGGTATGACATCATTTTTACCA
GTACGGACAGGTATCCTGATGCTGCCTGGTTCCTGCGTAAATCCGCAACCCTGATAATGGGAACCGGATTACTGGAAAGC
CTGACTGAACAGAAAAAAGTGCACGGTGTTATTACTGATTTCAGACGTATTTCTGGCTCTGAGGACCAGGCACAATACCG
GATCACACTTAAACCTTTCATTTCCCTGCTGGATAAACAGTTCCGTACACACCGTTTTTTCGTTAATAAATCAGTGCCTG
AGGTGGTGGAGCAAATCCTGACGGAACACGGGCTGAAAGGCTGGGAGTATGAGTTCAGCCTCAAAAGGACATATCCGAAG
CGCGAACAGATTAACCAGTATCAGGAAAGCGATCTGAGATTTATCCAGCGCCTGCTGGCAGAAGTGGGCATTTTTTACTT
CTTTACGTTGCACCCGGATGCGCAGACAGAGGTGATCCACTTCGGTGACGTCCAGGCGGCGTTGATATTTGATAAAACAC
TGCCCGTTAACAGCCCGTCCGGTATGAGTGACAGTGGCACAGACTCAGTATGGGCATTGAATGTGGAGCACCGGGTGGTG
GAAAGCCGTGTTATCACGAACGACTATAACCACCGCGAGGCCCAGAACATACTGATGTCCGTACCGGCGGACATGACCCG
TGGAGAAGGTTACGACACCAGTTATGGCGAGGTTTATCATTACCGGCCACGCCATACAGAAAGGGGCGATAAAATTGACC
CTGCGCCGGAGACGGCTAACTTCTGGGCCCGGCTCGATCACGAGCGTTTTCTGGCTGAGCAAACACGTATCACAGGTAAA
AGCACCGATGCCAGTCTGTTACCGGCACAGGTGCTGACTATTACTGACAGTACTCCACCAGTGTTGCCTGCACCGTTACA
GGAGCCGGTTCTGCTCACGCAGCTTCTGTTCACCGGTAGCCGGAAATCTGCGCTACAGGTGATGCTGAGTGCTGTTCCTT
ACAGCGAGATTGTCTGCTGGCGACCACCGTTACTTACTCGTCCGAAAATCACCGGGACCATGACGGCCCGTGTGACCAGT
GCAAAAGAAGGGGATATCTACGCCTGGCAGGATGCTTCAGGCATGTACCGGGTGAAATTTGATGCTGACCGTGATGATAA
AAATCCGGGACAGGAAAGTATGCCGGTACGTCTGGCCAAACCCTACAGTGGTGATGCTTATGGTTTTCATTTTCCGCTGA
TTCAGGGAACGGAAGTGGCCATTGCTTTTGAGGAAGGTGATCCGGATCGTCCCTATATAGCGCATGCCCTGCATGATTCA
CGTCATGTTGATCACGTTACCGATAAGAACGGTACGCGCAATGTTATCCGTACGCCGGCAAATAACAAGTTGCGGATGGA
GGACAAACGCGGTGAGGAGCATATCAAGCTCAGTACCGAGTATGGCGGTAAGACACAACTTAATCTTGGCCATAACGTTG
ATGCCTCCCGGGAACTCAGGGGAGAAGGCGCTGAGCTGAGGACTGATGACTGGATAAGTATCCGGGGAGGAAAAGGAATA
TTTATCAGTGCAGATATGCAGCCTCAGGCACAAGGAAAGATGCTGGATATGGATGAAGCTATCCGCCAATTGGAGCAGGC
ATTATCTCTGGCCCGAAGTATGGCGAAGGCCGCAACTGCTGCAAATGCTACTCAGGGAGATATAAGTTGTCAGCAACGGT
TGAATGCATCACTAACAGATCTCACTGCGCCGGGAATGCTGTTGCATGCTCCTGATGGGATTGGCATGGTTAGTGCGCGG
GCGTTACGAATTGCTTCAGGTAGTGAAAGCGTGGGCATAATGTCGGGAGATAACACTGATATTACTGCCGGGCAGTCATT
TACAGTTGTAGCAGAAGGTGCTGTTAGCCTGTTGAGCAGAAACCAGGGGATGCAACTGTTAGCAGCAAAAGGGCGGGTAA
ATATTCAGGCTCAAAGTGACGATTTGTCAATGAGTTCCCAACAGAATCTTGATATTCAAAGTTCTGAGGGCAAAGTGACT
GTTTCTGCTAATCAGGAACTGATACTGGCATGTGGTGGAGCTTATATCAAACTAAGCGGTGGAAATATTGAGCTGGGATG
CCCGGGACAGATTCTGTTAAAAAGCACGAATATGCAGAAGATGGGACCAACAAGTCTTGATATTGCGTCTGTTGAAATGC
CGCGAGGGTTTGGGGGCGGTTTTATTCTTACCGATGAAGCGGGAGTGCCGCAACCTTCGACGCCTTATCGATTAACAACG
GCTGAAGGTGATATTCTACAGGGGATCACCGATGAAAATGGTAAAACAGCGCCAGTCAACACCTCCATTCCGTCAGTAGT
AAAAGTTGAGTTTGGGAAGGTAAAAATTCATGGAGAAACAGAATGA