Detailed information of component protein


Summary

Component ID T6CP005162
Component protein type TssH
T6SS ID (Type) T6SS00463 experimental (Type i2)
Strain Escherichia coli 042
Replicon chromosome
Sequence Protein sequence (886 a.a.); Nucleotide sequence (2658 bp)
Reference
[1] Durand E, Zoued A, Spinelli S, et al. Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 2012 Apr 20;287(17):14157-68. doi: 10.1074/jbc.M111.338731. Epub 2012 Feb 27. PMID: 22371492
[2] Felisberto-Rodrigues C, Durand E, Aschtgen MS, et al. Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 2011 Nov;7(11):e1002386. doi: 10.1371/journal.ppat.1002386. Epub 2011 Nov 10. PMID: 22102820
[3] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558
[4] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962
[5] Zoued A, Durand E, Bebeacua C, et al. TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system. J Biol Chem. 2013 Sep 20;288(38):27031-27041. doi: 10.1074/jbc.M113.499772. Epub 2013 Aug 6. PMID: 23921384
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) EC042_RS24200
Coordinate (Strand) 4859340..4861997 (+)
NCBI ID WP_000431646.1
RefSeq NC_017626
Uniprot ID D3GUW1_ECO44
KEGG ID elo:EC042_4530
PDB ID 4HH6, 4HH5
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 1.5e-39 615..778
AAA_lid_9 PF17871.3 2.8e-31 381..472
ClpB_D2-small PF10431.11 7.6e-12 784..859
AAA PF00004.31 2.7e-08 241..353
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-885 MENSAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLLEQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAQSLNALLKQAWMVASLQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSQSQLQRLSPLLDAQSDECPETLQDAEPVLPQGDSVTFIGRPVGADTAGIPSGDLPPVLQGALDKFTRDITASAREGKIDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIVEGNVPESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQLSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVKVDEPDDETACLMLRSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARECELTGELLESRSDISRQSETHHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQEVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQTVIYRPLTPSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKPQALALAWSDEDGMVIELRQECAL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.5740 - -


  Protein sequence: 886 a.a.    

>T6CP005162 NC_017626:4859340-4861997 [Escherichia coli 042] [TssH]
MENSAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLLEQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSV
RSRPQLAQSLNALLKQAWMVASLQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSQSQLQRLSPLLDAQSDECPETLQ
DAEPVLPQGDSVTFIGRPVGADTAGIPSGDLPPVLQGALDKFTRDITASAREGKIDPVSGRDTEIRQMVDILSRRRKNNP
ILVGDPGVGKTALVEGLALRIVEGNVPESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQLSPAPVLLFIDEAHT
LIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVKVDEPDDETACLMLRSLKSRYAEHHN
VHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGE
RLSAIEQEENVLMTARDDLEQQYARECELTGELLESRSDISRQSETHHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWT
GVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQEVFLLVGPSGVGKTETALALADVMYG
GEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEG
REIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQTVIYRPLTPSAMRTIVEMKLAQV
CERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKPQALALAWSDEDGMVIELR
QECAL*

  Nucleotide sequence: 2658 bp    

>T6CP005162 NC_017626:4859340-4861997 [Escherichia coli 042] [TssH]
ATGGAAAATTCGGCAGCCCTGTTACGTCGTCTTAATCATTACTGTGCCCGTGCACTGGAAGGCGCAGCCTCCCTTTGCCA
GACCCGGGCCCATGCGGAAATCACCCCGGAGCACTGGTTACTGAAACTGCTGGAGCAGGGGGAAGGAGACCTTACCGTGC
TGGGCAGGCGTTATGACTGGGATATGGACGCGATATGGCAGTCGCTGCTCGGCTGGCTGGATAACCAGCCCCGTAGCGTA
CGCAGTCGCCCGCAGCTTGCGCAGTCCCTGAATGCTCTGCTGAAACAGGCCTGGATGGTGGCCTCATTACAGGGAGAAGA
ACATATCCGCAGCGTGCATCTGCTGGGTGCCCTGACGGAAAATCCGCACCTGGTTCGCTGTGACGGGCTGTGGCCTCTGC
TGACACTGAGTCAGAGCCAGTTGCAGCGTCTGTCCCCGCTGCTGGATGCGCAGTCTGATGAGTGTCCGGAGACGTTACAG
GATGCAGAGCCTGTGCTGCCTCAGGGAGACAGTGTGACCTTTATCGGGCGCCCTGTCGGTGCGGATACGGCAGGTATACC
GTCAGGTGACCTGCCGCCGGTGTTACAGGGTGCGCTGGATAAATTCACCCGGGACATCACGGCCAGCGCGAGAGAGGGGA
AAATTGACCCGGTATCGGGACGCGATACGGAAATCCGTCAGATGGTGGATATTCTCTCCCGCCGTCGCAAGAATAACCCG
ATTCTGGTGGGGGACCCCGGTGTGGGGAAAACGGCTCTGGTGGAAGGGCTCGCCCTGCGTATTGTGGAAGGTAACGTACC
AGAATCTCTCAGACCCGTCACCCTGCGCACCCTTGACCTCGGCCTGCTGCAGGCCGGTGCCGGCGTGAAAGGTGAATTTG
AACAGCGTCTGAAAAACGTGATTGATGCCGTGCAGCTGTCACCGGCTCCTGTACTGCTGTTTATAGATGAAGCCCATACC
CTTATCGGTGCCGGTAATCAGGCCGGTGGCGCGGATGCGGCCAACCTGCTGAAGCCTGCGCTGGCGCGAGGCGAACTGCG
CACCATTGCTGCCACCACCTGGTCTGAGTACAAACAATACCTGGAACGTGACGCGGCTCTGGAGCGGCGTTTTCAGATGG
TCAAAGTGGACGAGCCGGATGATGAGACGGCCTGTCTGATGCTGCGCTCCCTGAAATCCCGTTATGCGGAACATCATAAC
GTGCATATCACCGATGAGGCAGTACGTGCTGCCGTCACACTCTCGCGCCGTTATCTGACAGAACGTCAGTTACCGGACAA
GGCCGTTGACCTGCTTGATACTGCTGCTGCCCGTGTGCGGATGAGCCTCGACACGGTGCCGGAACAGCTGACCCGAATCC
GTTCACAGCTCGCTTCCCTCGGTATGGAAAAACAGGCACTGCTGGAAGATATTGCCGTTGGTCATCAGAATCACGGCGAA
CGCCTGTCTGCCATTGAGCAGGAAGAGAACGTGCTGATGACAGCACGTGATGACCTGGAGCAGCAGTACGCCCGTGAGTG
CGAACTCACCGGTGAGTTACTTGAAAGCCGCAGCGATATTTCCCGCCAGAGTGAGACACATCACCTGCAGCAGGCACTGC
ACGACATTCAGCAGAATCAGCCCCTGCTCAGTGTGGATGTTGACGTACGCACCGTTGCCGGTGTGGTCGCGGACTGGACC
GGAGTGCCGTTATCCTCACTGATGAAGGATGAACAGACGGAACTGCTGCATCTGGAAAAGGATATCGGCAGACGGGTGGT
CGGACAGGACGTGGCACTGGAATCCATTGCACAGCGTCTGCGTGCGGCGAAAACGGGTCTGACATCCGGTAACGGCCCCC
AGGAGGTGTTCCTGCTGGTCGGCCCCAGTGGTGTGGGAAAAACCGAAACGGCACTGGCGCTGGCAGATGTGATGTACGGC
GGCGAAAAATCACTGATTACCATCAATCTGTCGGAATATCAGGAGCCCCATACGGTTTCCCAGCTTAAGGGTTCACCGCC
CGGGTACGTGGGATACGGTCAGGGGGGTATTCTGACGGAAGCTGTACGTAAGCGCCCTTACAGCGTGGTGCTGCTGGATG
AAGTGGAAAAGGCCCACCGGGATGTGCTGAATCTGTTCTACCAGGTGTTTGACCGGGGCTTTATGCGCGACGGTGAGGGG
CGTGAAATTGACTTCCGTAATACGGTCATTCTGATGACCTCCAATCTGGGCAGTGACCTTCTGATGCAGCAACTGAGCGA
AAAGCCGGAGACAACGGAATCGGAGCTGCATGAGCTTATCCGTCCACTGCTGCGCGACCACTTCCAGCCAGCACTGCTTG
CCCGTTTCCAGACCGTGATTTACCGTCCGCTGACACCGTCTGCTATGCGCACCATTGTGGAGATGAAGCTTGCACAGGTC
TGCGAACGCCTGCACTGCCATTATGGGCTGAGCACATCGGTTGATGAACGTGTGTACGATGCCCTGACATCCGCCTGCCT
GCTGCCGGATACGGGCGCCCGGAATGTGGAGAGTCTGCTGAACCAGCAACTGCTGCCGGTACTGAGCCGGCAGTTGCTGA
GCCATATGGCCGCGAAGCAGAAACCGCAGGCGCTGGCTCTGGCATGGAGTGACGAAGACGGTATGGTGATTGAGCTGCGG
CAGGAATGCGCGTTATGA