SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005154
Component protein type	TssJ
T6SS ID (Type)	T6SS00485 (Type i1)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference	[1] Chaudhuri RR, Sebaihia M, Hobman JL, et al. Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. PLoS One. 2010 Jan 20;5(1):e8801. doi: 10.1371/journal.pone.0008801. PMID: 20098708

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	EC042_RS01180
Coordinate (Strand)	255998..256522 (-)
NCBI ID	WP_001013424.1
RefSeq	NC_017626
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	7.9e-24	45..164

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-174	MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFQAADYDQIATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIEPVESIGHHYRLLVHIRASAIEMKKEEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9991	pos: 18-19. LTG-CE.	MNGKAFLACVLMSVVLTG

Protein sequence: 175 a.a.

						>T6CP005154 NC_017626:c256522-255998 [Escherichia coli 042] [TssJ]
MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKF
QAADYDQIATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIEPVESIGHHYRLLV
HIRASAIEMKKEEN*					

Nucleotide sequence: 525 bp

						>T6CP005154 NC_017626:c256522-255998 [Escherichia coli 042] [TssJ]
ATGAACGGCAAAGCGTTTCTGGCCTGCGTTCTGATGAGCGTTGTGTTAACTGGCTGTGAAACAGCGAAAAAAATCAGCCA
GGTGATCCGCAATCCGGATATTCAGGTCGGAAAGCTGATGGATCAGTCAACCGAGCTGACCGTCACGCTGCTGACCGAGC
CGGACAGCAACCTGACGGCGGACGGTGAAGCCGCGCCGGTGGATGTCCAGTTGGTGTATCTGAGCGACGACTCCAAATTC
CAGGCCGCCGATTACGATCAGATTGCCACCACCGCGCTGCCCGACGTGCTGGGGAAGAACTATATCGATCACCAGGACTT
CAACCTGTTGCCGGACACCGTAAAAACACTGCCGCCGATCAAGCTGGATGAGAAAACCGGTTATATCGGTGTCATTGCCT
ATTTTTCAGACGACCAGGCCACAGAATGGAAACAAATTGAGCCGGTAGAAAGTATCGGCCACCACTATCGCCTGCTGGTG
CATATCCGCGCCAGTGCGATTGAGATGAAAAAAGAGGAAAACTGA					