Detailed information of component protein
Summary
| Component ID | T6CP005152 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS00485  (Type i1) |
| Strain | Escherichia coli 042 |
| Replicon | chromosome |
| Sequence | Protein sequence (258 a.a.); Nucleotide sequence (774 bp) |
| Reference |
Bioinformatics investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | EC042_RS01170 |
| Coordinate (Strand) | 253871..254644 (-) |
| NCBI ID | WP_001280269.1 |
| RefSeq | NC_017626 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 8.3e-55 | 37..237 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-216 | MSDISDMGEPSVFPMEPESGTSGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQAMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGFRGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRR |
| Transmembrane helix | 217-239 | LTIRHVLCGGLVVLAVFYLFYLL |
| Inside | 240-257 | RLDSQTQDILHQLNKLLR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9189 | - | - |
Protein sequence: 258 a.a.
>T6CP005152 NC_017626:c254644-253871 [Escherichia coli 042] [TssL]
MSDISDMGEPSVFPMEPESGTSGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQAMPEHLFAQVVTDVQAVEQLLQ
EQGYEPGVIVSFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFS
LGFRGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIRHVLCGGLVVLAVFYLFYLLR
LDSQTQDILHQLNKLLR*
MSDISDMGEPSVFPMEPESGTSGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQAMPEHLFAQVVTDVQAVEQLLQ
EQGYEPGVIVSFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFS
LGFRGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIRHVLCGGLVVLAVFYLFYLLR
LDSQTQDILHQLNKLLR*
Nucleotide sequence: 774 bp
>T6CP005152 NC_017626:c254644-253871 [Escherichia coli 042] [TssL]
ATGAGCGATATCAGCGATATGGGTGAACCGTCGGTTTTTCCCATGGAGCCGGAGAGCGGAACATCAGGACGTCAGTACCG
TCTCGCCCTTCGCGGCAACAGCCTGAACCCAATGATTGACGCCGCCACGCCGTTGCTCGGCATGGTGATGCGTCTGTCCA
CCATGAACAGCCAGGCCATGCCGGAACATCTGTTTGCCCAGGTGGTAACCGATGTCCAGGCTGTGGAACAACTACTCCAG
GAGCAGGGTTACGAGCCGGGCGTCATTGTTTCGTTCCGCTACATTCTCTGCACGTTTATTGACGAAGCGGCGCTGGGCAA
CGGCTGGTCCAACAAAAACGAATGGATAAAACAGTCGCTACTGGTTCATTTCCATAACGAAGCCTGGGGCGGCGAGAAAG
TGTTTATCCTGCTCGAACGCCTGATCCGGGAGCCGGTACGTTACCAGGATCTGCTGGAATTTTTATACCTCTGCTTTTCG
CTGGGCTTTCGCGGGCGCTACAAGGTTGCCATTCAGCAACAGGATGAATTTGAGCGGATCTACCAGCGCCTGCACCATGC
CCTGCACAAACTGCGCGGTGACGCGCCATTTCCGCTGCTTCACCAGAAGAACAACATACAGGGCGGGCGTTATCAGTTAA
TCCGGCGGCTGACCATCAGGCATGTACTGTGCGGCGGTCTTGTTGTGCTGGCCGTATTTTATCTGTTTTATCTGCTGCGT
CTGGACAGCCAGACACAGGATATTCTGCATCAGCTCAATAAATTACTCCGATAA
ATGAGCGATATCAGCGATATGGGTGAACCGTCGGTTTTTCCCATGGAGCCGGAGAGCGGAACATCAGGACGTCAGTACCG
TCTCGCCCTTCGCGGCAACAGCCTGAACCCAATGATTGACGCCGCCACGCCGTTGCTCGGCATGGTGATGCGTCTGTCCA
CCATGAACAGCCAGGCCATGCCGGAACATCTGTTTGCCCAGGTGGTAACCGATGTCCAGGCTGTGGAACAACTACTCCAG
GAGCAGGGTTACGAGCCGGGCGTCATTGTTTCGTTCCGCTACATTCTCTGCACGTTTATTGACGAAGCGGCGCTGGGCAA
CGGCTGGTCCAACAAAAACGAATGGATAAAACAGTCGCTACTGGTTCATTTCCATAACGAAGCCTGGGGCGGCGAGAAAG
TGTTTATCCTGCTCGAACGCCTGATCCGGGAGCCGGTACGTTACCAGGATCTGCTGGAATTTTTATACCTCTGCTTTTCG
CTGGGCTTTCGCGGGCGCTACAAGGTTGCCATTCAGCAACAGGATGAATTTGAGCGGATCTACCAGCGCCTGCACCATGC
CCTGCACAAACTGCGCGGTGACGCGCCATTTCCGCTGCTTCACCAGAAGAACAACATACAGGGCGGGCGTTATCAGTTAA
TCCGGCGGCTGACCATCAGGCATGTACTGTGCGGCGGTCTTGTTGTGCTGGCCGTATTTTATCTGTTTTATCTGCTGCGT
CTGGACAGCCAGACACAGGATATTCTGCATCAGCTCAATAAATTACTCCGATAA