SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005150
Component protein type	TssM
T6SS ID (Type)	T6SS00485 (Type i1)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (1146 a.a.); Nucleotide sequence (3438 bp)
Reference	[1] Chaudhuri RR, Sebaihia M, Hobman JL, et al. Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. PLoS One. 2010 Jan 20;5(1):e8801. doi: 10.1371/journal.pone.0008801. PMID: 20098708

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	EC042_RS01150
Coordinate (Strand)	245395..248832 (-)
NCBI ID	WP_122986661.1
RefSeq	NC_017626
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	2e-66	478..789
IcmF-related_N	PF14331.8	2.1e-59	181..426
IcmF_C	PF06744.14	8.5e-20	1019..1123

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-1	M
Transmembrane helix	2-16	LALAWIFLLVWIWWQ
Outside	17-30	GPKWTLYEQHWLAP
Transmembrane helix	31-53	LTNRWLATAVWGLIALIWLTWRV
Inside	54-421	MKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLRLRRHLDNRRYLWQLPWYMVIGPAGSGKSALLREGFPSDIIYTPESIRGTEYHPLITPRVGNQAVIFDVDGVLTSPGGDDLLHRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLTADKSRRETLVQNLRQQLQEIRQSLHCRLPVYVVLTRLDLLTGFAALFHSLDKKDRDAILGVTFTRRAHESDDWRSELGAFWQTWVQQVNLALSDLMLAQTGAAPRSAVFSFSRQMQGTGEIVTALLAALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLATWPLVETTPYFTRRLFPEVLLAEPNLAGENSVWLNSSRRR
Transmembrane helix	422-444	LTAFSACGAALAALLVGSWHHYY
Outside	445-1145	NQNWQSGVNVLAQAKAFMDVPPPQGTDEFGNLQLSLLNPVRDATLAYGDYRDRGFLADMGLYQGVRVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNNAPPESEEKLAVLRVLRMMEDKSGRNNEAVKQYMARRWSNEFHGQRDIQAQLMAHLDYALEHTDWHAQRQSGDSDAVSRWTPYDKPVINAQQELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNVFVAGNDEKLVIPQFLTRYGLQSYFVKQREGLVELTALDSWVLNLTQSVAYSEADREEIQRHITEQYISDYTATWRAGMDNLNVRDYEAMSALTDALEQIISGDQPFQRALTALRDNTHALTLSGKLDDKAREAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQLTELHRYLLAIQNSPVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGKLADQAWHVVMVEAVRYMEVDWRDNVVKPFNEQLADNYPFNPRATQDASLDSFERFFKPDGILDNFYKNNLRLFLENDLTFGDDGRVLIREDIRQQLDTAQKIRDIFFSQQNGLGAQFAVETVSLSGNKRRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGRAPHSIAFSGPWAQFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYRVHVDTEDNPFTGGLFSLFRLPDTLY

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9962	-	-

Protein sequence: 1146 a.a.

						>T6CP005150 NC_017626:c248832-245395 [Escherichia coli 042] [TssM]
MLALAWIFLLVWIWWQGPKWTLYEQHWLAPLTNRWLATAVWGLIALIWLTWRVMKRLQKLEKQQKQQREEEKDPLTVELH
RQQQYLDHWLLRLRRHLDNRRYLWQLPWYMVIGPAGSGKSALLREGFPSDIIYTPESIRGTEYHPLITPRVGNQAVIFDV
DGVLTSPGGDDLLHRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLTADKSRRETLVQNLRQQLQEIRQSLHCRLPVYV
VLTRLDLLTGFAALFHSLDKKDRDAILGVTFTRRAHESDDWRSELGAFWQTWVQQVNLALSDLMLAQTGAAPRSAVFSFS
RQMQGTGEIVTALLAALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLATWPLVETTPYFTRRLFPEV
LLAEPNLAGENSVWLNSSRRRLTAFSACGAALAALLVGSWHHYYNQNWQSGVNVLAQAKAFMDVPPPQGTDEFGNLQLSL
LNPVRDATLAYGDYRDRGFLADMGLYQGVRVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNNAPPESEEKLAVLRVLRMME
DKSGRNNEAVKQYMARRWSNEFHGQRDIQAQLMAHLDYALEHTDWHAQRQSGDSDAVSRWTPYDKPVINAQQELSKLPIY
QRVYQTLRTKALSVLPADLNLRDQVGPTFDNVFVAGNDEKLVIPQFLTRYGLQSYFVKQREGLVELTALDSWVLNLTQSV
AYSEADREEIQRHITEQYISDYTATWRAGMDNLNVRDYEAMSALTDALEQIISGDQPFQRALTALRDNTHALTLSGKLDD
KAREAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQLTELHRYLLAIQNSPVPGKSALKAVQLRLDQNSSD
PIFATRQMAKTLPAPLNRWVGKLADQAWHVVMVEAVRYMEVDWRDNVVKPFNEQLADNYPFNPRATQDASLDSFERFFKP
DGILDNFYKNNLRLFLENDLTFGDDGRVLIREDIRQQLDTAQKIRDIFFSQQNGLGAQFAVETVSLSGNKRRSVLNLDGQ
LVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGRAPHSIAFSGPWAQFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYR
VHVDTEDNPFTGGLFSLFRLPDTLY*					

Nucleotide sequence: 3438 bp

						>T6CP005150 NC_017626:c248832-245395 [Escherichia coli 042] [TssM]
CTGCTGGCGCTGGCATGGATTTTTCTGCTGGTGTGGATCTGGTGGCAAGGTCCGAAATGGACGCTCTATGAGCAGCACTG
GCTGGCTCCTCTAACAAACCGCTGGCTGGCGACCGCCGTCTGGGGGCTTATCGCTCTGATCTGGCTCACCTGGCGGGTAA
TGAAGCGTCTGCAAAAGCTGGAAAAACAGCAGAAACAGCAGCGGGAGGAAGAAAAAGATCCGTTGACCGTGGAACTCCAC
CGCCAGCAGCAATATCTGGATCACTGGCTGCTGCGCCTGCGCCGCCATCTGGATAACCGCCGTTATCTGTGGCAGTTGCC
GTGGTATATGGTCATTGGTCCTGCGGGTAGCGGTAAAAGCGCTCTGCTGCGCGAGGGCTTTCCATCTGACATTATTTACA
CGCCGGAAAGCATCCGGGGTACGGAATACCATCCGCTGATCACACCGCGAGTGGGCAACCAAGCGGTGATTTTCGATGTT
GACGGCGTACTGACCTCGCCCGGCGGGGATGATCTGCTCCACCGCCGCCTGCGCGAACACTGGCTGGGCTGGCTGATGCA
AACGCGCGCGCGCCAGCCGCTCAACGGCCTGATCCTGACGCTCGATCTTCCCGATCTGCTGACGGCGGATAAATCCCGCC
GTGAGACACTGGTACAAAATTTGCGCCAGCAACTTCAGGAGATCCGCCAGAGTCTGCACTGCCGTCTGCCCGTTTACGTG
GTGCTGACACGGCTGGATCTGCTGACCGGCTTTGCCGCGCTGTTCCATTCACTGGATAAAAAAGACCGCGATGCGATCCT
CGGCGTCACGTTTACCCGCCGCGCCCATGAAAGTGACGACTGGCGCAGCGAACTGGGGGCTTTCTGGCAGACGTGGGTAC
AACAGGTGAACCTGGCGCTGTCGGATCTGATGCTCGCACAAACCGGTGCTGCTCCCCGCAGCGCCGTGTTCAGCTTCTCC
CGTCAGATGCAGGGAACAGGAGAAATCGTCACCGCACTGCTCGCCGCATTGCTGGACGGTGAGAACATGGATGTAATGCT
GCGTGGCGTCTGGCTCACATCATCGCTACAGCGTGGCCAGGTGGATGATATTTTCACGCAGTCCGCCGCCCGCCAGTACG
GGCTGGGTAACAGCTCGCTGGCAACCTGGCCTCTGGTGGAGACGACGCCGTATTTTACTCGCCGCCTCTTCCCTGAAGTC
CTGCTGGCTGAGCCGAACCTGGCGGGTGAAAACAGCGTCTGGCTGAACAGCTCCCGGCGCAGGCTGACCGCCTTTTCCGC
CTGTGGCGCGGCGCTGGCGGCATTGCTGGTCGGAAGCTGGCACCATTATTACAATCAGAACTGGCAGTCCGGCGTTAACG
TACTGGCACAGGCTAAAGCCTTTATGGACGTACCACCACCGCAAGGAACGGATGAATTCGGCAATCTGCAACTGTCGTTG
CTTAATCCGGTACGCGATGCCACCCTGGCCTATGGCGATTACCGCGATCGCGGTTTTCTGGCGGATATGGGATTGTACCA
GGGCGTCCGCGTAGGGCCGTATGTGGAGCAAACCTACATTCAGCTTCTTGAGCAGCGTTATCTCCCCTCGTTAATGAACG
GCCTGATCCGGGATCTAAACAATGCCCCGCCAGAGAGCGAAGAAAAGCTCGCCGTGCTGCGCGTACTGCGCATGATGGAA
GACAAAAGTGGGCGCAACAACGAGGCGGTAAAACAGTACATGGCGCGGCGCTGGAGCAATGAATTTCACGGCCAGCGCGA
TATTCAGGCGCAACTGATGGCGCATCTGGACTATGCGCTGGAGCACACCGACTGGCACGCGCAGCGCCAGAGCGGTGACA
GCGATGCTGTCAGCCGCTGGACCCCCTATGATAAACCGGTCATTAATGCGCAGCAGGAACTGAGCAAGCTGCCCATATAC
CAGCGTGTCTACCAGACCCTGCGCACCAAAGCATTAAGCGTGTTGCCCGCCGATTTGAATTTGCGCGACCAGGTTGGTCC
CACCTTCGACAACGTGTTCGTCGCCGGTAATGATGAAAAACTGGTGATCCCGCAGTTCCTCACCCGCTATGGACTGCAAA
GCTATTTTGTCAAACAGCGTGAGGGCCTCGTTGAGCTGACCGCGCTGGATTCGTGGGTACTGAACCTGACGCAAAGCGTC
GCCTACAGCGAGGCCGACCGTGAAGAGATCCAGCGCCATATCACCGAACAGTACATCAGTGACTATACCGCCACCTGGCG
TGCCGGAATGGATAACCTCAACGTCCGTGACTATGAGGCCATGTCGGCGCTGACCGACGCGCTGGAGCAGATTATCAGCG
GCGATCAGCCATTCCAGCGTGCGCTGACGGCGCTGCGCGATAATACCCACGCGCTGACGCTCTCCGGCAAACTGGATGAT
AAGGCGAGGGAAGCGGCAATAAATGAGATGGATTACCGCCTGTTATCCCGGCTGGGGCATGAGTTCGCACCGGAAAACAG
CGCACTGGAGGAGCAAAAGGACAAGGCGAGTACGCTACAGGCCGTGTACCAGCAACTGACCGAGCTGCACCGTTACCTGC
TGGCGATCCAGAACTCGCCAGTGCCGGGGAAATCGGCGCTGAAAGCAGTACAGCTACGTCTGGATCAAAACAGCAGCGAT
CCAATCTTCGCTACCCGCCAGATGGCAAAAACTCTCCCTGCACCGCTTAACCGCTGGGTAGGTAAGCTCGCGGATCAGGC
CTGGCATGTGGTGATGGTGGAAGCCGTTCGTTACATGGAAGTGGACTGGCGCGACAATGTAGTGAAACCCTTCAACGAGC
AGCTTGCCGATAACTATCCGTTTAATCCGCGCGCCACACAGGATGCCTCACTGGATTCGTTTGAACGTTTCTTTAAACCG
GATGGCATTCTGGACAATTTCTACAAGAACAACCTGCGCCTGTTCCTTGAAAACGATCTGACCTTTGGCGACGACGGCAG
AGTGTTAATCCGTGAAGATATCCGGCAGCAACTGGATACCGCGCAGAAAATCCGCGACATCTTCTTCAGCCAGCAGAACG
GGCTGGGCGCACAGTTTGCCGTGGAAACCGTATCGCTTTCCGGCAATAAGCGGCGCAGCGTACTTAACCTGGACGGCCAG
TTAGTGGACTACAGCCAGGGACGCAACTACACCGCCCATCTGGTCTGGCCGAACAACATGCGTGAAGGCAATGAAAGCAA
GCTGACGCTGATTGGCACCAGCGGCAGAGCACCGCACAGTATCGCGTTCAGTGGACCGTGGGCGCAGTTCCGCCTGTTCG
GCGCGGGCCAGTTGACCAATGTGACCAGTGACACCTTTAACGTGCGCTTTAACGTGGACGGCGGCGCAATGGTTTACCGG
GTGCATGTGGATACCGAAGATAACCCGTTCACCGGCGGTCTGTTCAGCCTGTTCCGTTTACCGGATACGTTGTATTAA					