SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005143
Component protein type	TssF
T6SS ID (Type)	T6SS00464 (Type i4b)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (601 a.a.); Nucleotide sequence (1803 bp)
Reference	[1] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558 [2] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	EC042_RS24380
Coordinate (Strand)	4895974..4897776 (+)
NCBI ID	WP_001390300.1
RefSeq	NC_017626
Uniprot ID	A0A222QQF7_ECOLX, D3GUZ3_ECO44, A0A2H3M0V1_ECOLX, B7LFR1_ECO55
KEGG ID	elo:EC042_4566, eck:EC55989_3294
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	2.1e-141	2..596

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-600	MNKLLSYYQKELAFLRKQGIEFAKKYPKVARRLGYNQGGSEDPHVQRLTESFAFLSSKIHQRLDEDLPELMEALLRNLAPQFLYNYPSSCIVSFEPDRKSSGITSTYRVPSGTSLYSRYKYKTICQFRTVYPLTIVPATVNKARIHYDNSSSLWKIELNLDVFDGANFSLDFVRFYLNGPATVTGIIYTLLCDEVDNIVLQRNNDVLYDISANISGAGFYESEVTLPQKGNVSYIHNLILDYFSFPERFNFIDIEFDSGIELCSGDSVRIEITLKSSAEKYEPDRIERNVSERLFKVNCSPVVNLFEKKTEPLRLSDGQDEYQVNTDLYSRNEVLIWDINKVTLHRYENDIVKQFPLKNLFGLEHSVIKQSCNIFWHSSCYRSSSERGNFEESVYIRLSDDGGAQSCIMTGDVLSIDAICTNGNIPSSINNGDPDGDFDCDIDIAQLRITALNRPSRMLPAMALKGMNWRIVSQLSLNFVLLSGTDGAMKLREMLSIYNYNNNHSISIFIEWIKELQITPVSSRLPGIYPPVTARGIDITVTLSKDADMHPECFMFCSFLDHFLGLHAPVNSFTRMITVIEHEEHTRRVWPIRAGKLTWL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9898	-	-

Protein sequence: 601 a.a.

						>T6CP005143 NC_017626:4895974-4897776 [Escherichia coli 042] [TssF]
MNKLLSYYQKELAFLRKQGIEFAKKYPKVARRLGYNQGGSEDPHVQRLTESFAFLSSKIHQRLDEDLPELMEALLRNLAP
QFLYNYPSSCIVSFEPDRKSSGITSTYRVPSGTSLYSRYKYKTICQFRTVYPLTIVPATVNKARIHYDNSSSLWKIELNL
DVFDGANFSLDFVRFYLNGPATVTGIIYTLLCDEVDNIVLQRNNDVLYDISANISGAGFYESEVTLPQKGNVSYIHNLIL
DYFSFPERFNFIDIEFDSGIELCSGDSVRIEITLKSSAEKYEPDRIERNVSERLFKVNCSPVVNLFEKKTEPLRLSDGQD
EYQVNTDLYSRNEVLIWDINKVTLHRYENDIVKQFPLKNLFGLEHSVIKQSCNIFWHSSCYRSSSERGNFEESVYIRLSD
DGGAQSCIMTGDVLSIDAICTNGNIPSSINNGDPDGDFDCDIDIAQLRITALNRPSRMLPAMALKGMNWRIVSQLSLNFV
LLSGTDGAMKLREMLSIYNYNNNHSISIFIEWIKELQITPVSSRLPGIYPPVTARGIDITVTLSKDADMHPECFMFCSFL
DHFLGLHAPVNSFTRMITVIEHEEHTRRVWPIRAGKLTWL*					

Nucleotide sequence: 1803 bp

						>T6CP005143 NC_017626:4895974-4897776 [Escherichia coli 042] [TssF]
ATGAATAAACTATTATCTTACTATCAGAAAGAACTCGCTTTTCTTAGAAAACAAGGTATTGAGTTTGCAAAAAAATATCC
AAAGGTAGCCCGGCGTCTTGGATACAATCAGGGGGGGTCTGAAGATCCACATGTTCAGAGATTAACGGAGTCTTTTGCTT
TCTTAAGTTCAAAAATTCATCAGAGATTAGATGAAGATTTACCCGAGTTAATGGAAGCATTACTTCGTAATCTTGCTCCC
CAATTTTTGTATAACTATCCTTCATCATGTATTGTATCATTTGAACCAGACAGAAAATCCAGTGGAATAACTTCAACTTA
TAGAGTTCCTTCTGGCACATCGCTATATTCCAGATATAAATATAAAACCATCTGTCAGTTTCGGACGGTATATCCACTGA
CTATTGTCCCTGCAACGGTTAATAAAGCAAGAATTCACTATGATAATTCGTCCTCCTTATGGAAAATTGAATTGAATTTA
GACGTTTTCGACGGAGCTAATTTTTCTCTTGATTTTGTAAGATTTTATTTGAATGGTCCGGCAACGGTTACTGGTATAAT
ATATACATTATTGTGCGATGAGGTCGATAATATCGTTTTGCAGAGAAATAATGATGTGTTATATGATATCAGTGCTAATA
TATCCGGTGCCGGATTTTATGAGAGTGAAGTAACACTTCCACAAAAAGGGAACGTTTCTTATATACATAACCTCATACTG
GACTATTTTTCTTTTCCAGAACGATTTAACTTCATTGATATAGAATTTGACTCCGGAATCGAATTATGCTCAGGGGATTC
TGTGAGAATAGAAATAACATTAAAAAGCAGTGCAGAAAAGTATGAGCCAGACAGAATAGAAAGGAATGTTTCTGAAAGAC
TTTTTAAAGTAAACTGTTCTCCCGTTGTCAATCTCTTCGAGAAGAAAACTGAACCCCTACGATTATCTGATGGCCAGGAT
GAGTATCAGGTAAATACCGATTTATATTCACGTAATGAAGTCTTAATCTGGGATATAAATAAAGTGACATTGCACAGATA
TGAAAATGATATAGTCAAGCAGTTTCCGTTAAAAAATTTATTTGGACTTGAACATTCTGTCATTAAACAATCGTGCAATA
TATTCTGGCATAGTTCATGTTATCGCTCGTCTTCTGAAAGAGGGAACTTTGAGGAAAGTGTATATATTCGTCTGAGCGAT
GATGGCGGAGCCCAGTCATGTATCATGACTGGGGATGTCCTTTCAATTGATGCAATATGTACAAATGGTAATATTCCATC
ATCAATTAATAATGGTGATCCTGATGGTGATTTTGATTGTGATATAGATATTGCTCAGCTGCGAATAACTGCGCTGAACC
GTCCATCAAGAATGTTGCCGGCTATGGCATTAAAAGGAATGAACTGGAGAATTGTTTCTCAGTTATCCCTTAATTTTGTG
TTGCTCAGTGGTACTGATGGGGCAATGAAACTGAGAGAAATGCTATCCATATATAACTACAACAATAACCACTCAATATC
AATTTTTATTGAATGGATTAAAGAGTTACAAATCACACCAGTTTCATCCCGTTTACCCGGCATATATCCGCCTGTAACTG
CTCGTGGAATTGATATTACCGTAACTCTTTCAAAGGATGCAGATATGCACCCTGAATGTTTCATGTTTTGCTCTTTCCTT
GATCACTTTCTTGGGCTACATGCCCCTGTCAATAGCTTTACTCGCATGATTACTGTCATTGAACATGAGGAGCACACTCG
TCGTGTATGGCCGATAAGAGCAGGAAAGTTAACATGGCTTTGA					