SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005141
Component protein type	TssB
T6SS ID (Type)	T6SS00464 (Type i4b)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (159 a.a.); Nucleotide sequence (477 bp)
Reference	[1] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558 [2] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	EC042_RS24360
Coordinate (Strand)	4893076..4893552 (+)
NCBI ID	WP_000072549.1
RefSeq	NC_017626
Uniprot ID	A0A0E0XW46_ECO1C, A0A222QPX0_ECOLX, D3GUY9_ECO44, A0A2H3MMK8_ECOLX, B7LFR5_ECO55
KEGG ID	elo:EC042_4562, esl:O3K_04355, eck:EC55989_3298
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	3.8e-46	9..147

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-158	MSNTQHKLDKTRPPRVQITYDVETGDAETAKELPLVIGVLGEYSSSEKPLRERKFISIDKDNFDEVMASMSPTAHFMVDSVIPGNEGKLDVELRFSCKDDFSPDNVIQQVECLRKLSELRLHLCDLRNRAASNEKLKEKLQELLVTTNGNPENDESKE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9956	-	-

Protein sequence: 159 a.a.

						>T6CP005141 NC_017626:4893076-4893552 [Escherichia coli 042] [TssB]
MSNTQHKLDKTRPPRVQITYDVETGDAETAKELPLVIGVLGEYSSSEKPLRERKFISIDKDNFDEVMASMSPTAHFMVDS
VIPGNEGKLDVELRFSCKDDFSPDNVIQQVECLRKLSELRLHLCDLRNRAASNEKLKEKLQELLVTTNGNPENDESKE*					

Nucleotide sequence: 477 bp

						>T6CP005141 NC_017626:4893076-4893552 [Escherichia coli 042] [TssB]
ATGAGCAATACACAACATAAACTTGATAAAACCCGCCCCCCACGAGTACAGATAACGTATGATGTTGAAACCGGAGATGC
TGAAACTGCGAAAGAGTTACCCCTTGTCATCGGGGTTTTGGGTGAGTACTCTTCGTCAGAAAAACCATTGAGAGAACGAA
AATTCATTAGTATAGATAAAGACAATTTTGATGAAGTTATGGCATCAATGAGTCCGACTGCTCATTTTATGGTGGACAGT
GTTATCCCGGGAAATGAAGGAAAACTGGATGTCGAACTGAGGTTTAGTTGCAAAGATGATTTTTCTCCCGATAATGTGAT
TCAACAGGTTGAGTGTCTAAGAAAATTATCGGAATTGAGGTTGCATCTTTGTGACCTACGAAACAGGGCTGCAAGTAATG
AGAAACTGAAAGAAAAACTTCAGGAATTGCTGGTGACTACTAATGGCAATCCTGAAAACGATGAAAGTAAGGAATAA					