Detailed information of component protein


Summary

Component ID T6CP005070
Component protein type TssL
T6SS ID (Type) T6SS00069 insolico (Type i3)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (553 a.a.); Nucleotide sequence (1659 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS18260
Coordinate (Strand) 454160..455818 (+)
NCBI ID WP_004190463.1
RefSeq NC_006349
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.3e-41 121..316
OmpA PF00691.22 2.8e-09 376..483
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-294 MLDRAAANPDLVTRVPTLSSLSSAAMTSSAVSTTGTTNTVASGGAAAGAPSFAPPAADAFPQADGAPRNPAVLQFPVPGGAPAPADARVAAPVVYSAQGEQAAIMKAGLQQASWNNPFVSHALPAVLQLQRHLAAGPLNQAAIRTQLGLEVRLYRERLAASGCEWEQIRDASYLLCTYLDETVNDAAREHAQVVYDGERSLLVEFHDDAWGGEDAFADLSRWMKTEPPPIPLLSFYELILSLGWQGRYRVLDRVDVLLQDLRSQLHALIWHHVPPEPLGTELVAPAKRRRSWWT
Transmembrane helix 295-317 AGRAAAVALGVLVLAYGAISFWL
Outside 318-552 DSQGRPIRNALAAWMPPTRTINIAETLPPPLPQILTEGWLTAYKHPQGWLLVFKSDGAFDVGKANVRADFMHNIERLGLAFAPWPGDLEVIGHTDSRPIRTSEFPDNQALSEARARNVADELRKTALPGGARAPENAVQRNIEYSGRGDAQPIDTAKTAAAYERNRRVDVLWKVIPDGAQQSGRSLNLQQPEKPGQVPMRPAMPEGVEIAPDGQLPYATSTTMPATRPTTEGRQP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
TAT(Tat/SPI) 0.5432 pos: 59-60. ADA-FP. MLDRAAANPDLVTRVPTLSSLSSAAMTSSAVSTTGTTNTVASGGAAAGAPSFAPPAADA


  Protein sequence: 553 a.a.    

>T6CP005070 NC_006349:454160-455818 [Burkholderia mallei ATCC 23344] [TssL]
MLDRAAANPDLVTRVPTLSSLSSAAMTSSAVSTTGTTNTVASGGAAAGAPSFAPPAADAFPQADGAPRNPAVLQFPVPGG
APAPADARVAAPVVYSAQGEQAAIMKAGLQQASWNNPFVSHALPAVLQLQRHLAAGPLNQAAIRTQLGLEVRLYRERLAA
SGCEWEQIRDASYLLCTYLDETVNDAAREHAQVVYDGERSLLVEFHDDAWGGEDAFADLSRWMKTEPPPIPLLSFYELIL
SLGWQGRYRVLDRVDVLLQDLRSQLHALIWHHVPPEPLGTELVAPAKRRRSWWTAGRAAAVALGVLVLAYGAISFWLDSQ
GRPIRNALAAWMPPTRTINIAETLPPPLPQILTEGWLTAYKHPQGWLLVFKSDGAFDVGKANVRADFMHNIERLGLAFAP
WPGDLEVIGHTDSRPIRTSEFPDNQALSEARARNVADELRKTALPGGARAPENAVQRNIEYSGRGDAQPIDTAKTAAAYE
RNRRVDVLWKVIPDGAQQSGRSLNLQQPEKPGQVPMRPAMPEGVEIAPDGQLPYATSTTMPATRPTTEGRQP*

  Nucleotide sequence: 1659 bp    

>T6CP005070 NC_006349:454160-455818 [Burkholderia mallei ATCC 23344] [TssL]
ATGCTCGATCGCGCGGCGGCGAATCCGGATCTCGTGACGCGCGTGCCGACGCTGTCGTCGCTGTCGAGCGCGGCGATGAC
GAGCTCCGCCGTATCGACCACCGGCACGACGAACACGGTGGCGTCGGGCGGCGCGGCCGCGGGCGCGCCGAGCTTCGCGC
CGCCGGCCGCCGACGCGTTTCCCCAAGCCGACGGCGCGCCCCGCAACCCCGCGGTGCTGCAATTCCCGGTGCCGGGCGGC
GCGCCCGCGCCCGCCGACGCGCGGGTGGCCGCGCCCGTCGTCTATAGCGCGCAGGGCGAGCAGGCCGCGATCATGAAAGC
AGGCCTGCAGCAGGCGAGCTGGAACAACCCGTTCGTGTCGCACGCGCTGCCCGCGGTGCTGCAACTGCAGCGCCACCTCG
CGGCCGGCCCGCTCAATCAGGCCGCGATCCGCACGCAGCTCGGCCTCGAGGTGCGGCTCTACCGCGAGCGGCTCGCCGCC
TCCGGCTGCGAATGGGAGCAGATCCGCGACGCATCGTACCTGCTCTGCACGTATCTCGACGAAACCGTCAACGACGCGGC
GCGCGAGCACGCGCAAGTCGTCTACGACGGCGAGCGCAGCCTGCTCGTCGAATTCCACGACGACGCGTGGGGCGGCGAGG
ACGCGTTCGCCGACCTGTCGCGCTGGATGAAGACCGAGCCGCCGCCGATTCCGCTTCTGTCGTTCTACGAACTGATCCTG
TCGCTCGGCTGGCAGGGCCGCTACCGCGTGCTCGACCGCGTCGACGTGCTGCTGCAGGATCTGCGCTCGCAACTGCACGC
GCTGATCTGGCATCACGTGCCGCCCGAGCCGCTCGGCACCGAGCTCGTCGCGCCCGCGAAGCGGCGCCGCTCGTGGTGGA
CGGCCGGGCGCGCGGCGGCCGTCGCGCTCGGCGTGCTGGTGCTCGCGTACGGCGCGATCAGCTTCTGGCTCGATTCGCAG
GGCCGCCCGATCCGCAACGCGCTCGCCGCGTGGATGCCGCCCACGCGCACGATCAACATCGCCGAGACGCTGCCGCCGCC
GCTGCCGCAGATTCTCACCGAAGGGTGGCTCACCGCGTACAAGCATCCGCAAGGATGGCTGCTCGTGTTCAAGAGCGACG
GCGCGTTCGACGTTGGCAAGGCGAACGTGCGGGCGGACTTCATGCACAACATCGAGCGGCTCGGCCTCGCGTTCGCGCCG
TGGCCGGGCGACCTCGAGGTGATCGGCCACACCGATTCGCGGCCGATCCGCACGAGCGAGTTCCCGGACAACCAGGCGCT
GTCCGAAGCGCGGGCGCGCAACGTCGCCGACGAACTGCGCAAGACCGCGCTGCCGGGCGGCGCGCGCGCGCCGGAGAACG
CGGTGCAGCGCAACATCGAGTACTCGGGGCGCGGCGACGCGCAGCCGATCGACACCGCGAAGACGGCCGCCGCGTACGAG
CGCAACCGCCGCGTCGACGTGCTGTGGAAGGTGATTCCCGACGGCGCGCAGCAATCGGGCCGCAGCCTGAACCTGCAGCA
GCCGGAGAAGCCCGGGCAGGTGCCGATGCGTCCGGCGATGCCGGAGGGCGTGGAGATCGCGCCTGACGGGCAACTGCCGT
ATGCGACGTCAACCACGATGCCAGCAACGCGACCGACCACGGAGGGCCGTCAGCCATGA