Detailed information of component protein
Summary
| Component ID | T6CP004626 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00444  (Type Unknown) |
| Strain | Methylomonas methanica MC09 |
| Replicon | chromosome |
| Sequence | Protein sequence (158 a.a.); Nucleotide sequence (474 bp) |
| Reference | - |
Bioinformatics investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | METME_RS17295 |
| Coordinate (Strand) | 3816088..3816561 (-) |
| NCBI ID | WP_013820042.1 |
| RefSeq | NC_015572 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.6e-37 | 33..149 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-157 | MKRYISLVLLNAVLLGCASEPPVQPPPPPPTIVNLQIETSADLNADINGNGAPLMLRIYELREQSNFNSADFFAIFNNEKATLAADLVRKQELLLQPGETKSLSLNPDNDVQSIGFFAGFRQLDTAQWRAVASVKEHQTQSVQIKLKNNQLTVEVPN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9991 | pos: 16-17. LLG-CA. | MKRYISLVLLNAVLLG |
Protein sequence: 158 a.a.
>T6CP004626 NC_015572:c3816561-3816088 [Methylomonas methanica MC09] [TssJ]
MKRYISLVLLNAVLLGCASEPPVQPPPPPPTIVNLQIETSADLNADINGNGAPLMLRIYELREQSNFNSADFFAIFNNEK
ATLAADLVRKQELLLQPGETKSLSLNPDNDVQSIGFFAGFRQLDTAQWRAVASVKEHQTQSVQIKLKNNQLTVEVPN*
MKRYISLVLLNAVLLGCASEPPVQPPPPPPTIVNLQIETSADLNADINGNGAPLMLRIYELREQSNFNSADFFAIFNNEK
ATLAADLVRKQELLLQPGETKSLSLNPDNDVQSIGFFAGFRQLDTAQWRAVASVKEHQTQSVQIKLKNNQLTVEVPN*
Nucleotide sequence: 474 bp
>T6CP004626 NC_015572:c3816561-3816088 [Methylomonas methanica MC09] [TssJ]
ATGAAACGATATATATCTCTGGTTTTGCTGAATGCCGTGTTGCTGGGTTGCGCCAGCGAACCGCCGGTTCAGCCCCCGCC
CCCGCCGCCGACTATCGTGAACTTGCAAATAGAAACGTCTGCCGATCTGAATGCCGATATTAATGGTAACGGCGCGCCGC
TTATGTTACGGATATACGAATTGCGCGAACAAAGCAATTTCAATTCCGCCGATTTTTTTGCGATCTTCAACAATGAAAAA
GCCACGCTGGCGGCTGATTTAGTGCGTAAACAAGAGCTGCTGTTACAACCCGGCGAAACCAAAAGCCTGAGCCTGAATCC
GGATAACGATGTGCAATCCATCGGTTTTTTTGCCGGTTTCCGGCAACTGGACACCGCGCAATGGCGAGCCGTGGCAAGTG
TCAAAGAGCACCAAACCCAATCGGTGCAAATCAAATTGAAAAATAACCAATTAACGGTGGAAGTTCCGAATTGA
ATGAAACGATATATATCTCTGGTTTTGCTGAATGCCGTGTTGCTGGGTTGCGCCAGCGAACCGCCGGTTCAGCCCCCGCC
CCCGCCGCCGACTATCGTGAACTTGCAAATAGAAACGTCTGCCGATCTGAATGCCGATATTAATGGTAACGGCGCGCCGC
TTATGTTACGGATATACGAATTGCGCGAACAAAGCAATTTCAATTCCGCCGATTTTTTTGCGATCTTCAACAATGAAAAA
GCCACGCTGGCGGCTGATTTAGTGCGTAAACAAGAGCTGCTGTTACAACCCGGCGAAACCAAAAGCCTGAGCCTGAATCC
GGATAACGATGTGCAATCCATCGGTTTTTTTGCCGGTTTCCGGCAACTGGACACCGCGCAATGGCGAGCCGTGGCAAGTG
TCAAAGAGCACCAAACCCAATCGGTGCAAATCAAATTGAAAAATAACCAATTAACGGTGGAAGTTCCGAATTGA