Detailed information of component protein


Summary

Component ID T6CP004624
Component protein type TssL
T6SS ID (Type) T6SS00444 insolico (Type Unknown)
Strain Methylomonas methanica MC09
Replicon chromosome
Sequence Protein sequence (463 a.a.); Nucleotide sequence (1389 bp)
Reference
-
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) METME_RS17270
Coordinate (Strand) 3808637..3810025 (-)
NCBI ID WP_013820037.1
RefSeq NC_015572
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.6e-64 80..280
OmpA PF00691.22 1.6e-14 357..455
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-261 MSENDPFGPFGDDDRTVIRPMPGGRRPAPAPAQTPPVQPAQPAPAYAAPPPPLNPAPAPMPGFNPSAPVALPTTEFQQNPMVAAALSMISLASRLRQTAVYPAVDELRQRLSAEVSGFENKLLQTGIGADQVRMASYALCSFLDETILNTPWGSQSNWGHQSLLISFHKEAWGGEKFFDIIGMLVKQPAQNLNLIELAYLFLSLGFQGKFRILSNGLNELEKYRLELYQLIARVKGDYERELSPRWQGLRDVRNALVRYVP
Transmembrane helix 262-284 LWVVGAVLGVALILTYIGFVLAL
Inside 285-462 NARSDQLFAELFGLGKQPIEIAQAAAVIPLPAPVPKPVVPERRERFKQLLAKEIAADMVEVVDDKLLRIRNSFASGSDQLKPQFEPMLKKISDELVAGNDRVLVTGHTDNVPIRSARFPSNWHLSTARAKSVADDMLKAAPALQGKIRSEGRADSEGLVPNDTPEHRAINRRVDLLIQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.4784 - -


  Protein sequence: 463 a.a.    

>T6CP004624 NC_015572:c3810025-3808637 [Methylomonas methanica MC09] [TssL]
MSENDPFGPFGDDDRTVIRPMPGGRRPAPAPAQTPPVQPAQPAPAYAAPPPPLNPAPAPMPGFNPSAPVALPTTEFQQNP
MVAAALSMISLASRLRQTAVYPAVDELRQRLSAEVSGFENKLLQTGIGADQVRMASYALCSFLDETILNTPWGSQSNWGH
QSLLISFHKEAWGGEKFFDIIGMLVKQPAQNLNLIELAYLFLSLGFQGKFRILSNGLNELEKYRLELYQLIARVKGDYER
ELSPRWQGLRDVRNALVRYVPLWVVGAVLGVALILTYIGFVLALNARSDQLFAELFGLGKQPIEIAQAAAVIPLPAPVPK
PVVPERRERFKQLLAKEIAADMVEVVDDKLLRIRNSFASGSDQLKPQFEPMLKKISDELVAGNDRVLVTGHTDNVPIRSA
RFPSNWHLSTARAKSVADDMLKAAPALQGKIRSEGRADSEGLVPNDTPEHRAINRRVDLLIQ*

  Nucleotide sequence: 1389 bp    

>T6CP004624 NC_015572:c3810025-3808637 [Methylomonas methanica MC09] [TssL]
ATGAGTGAAAACGATCCCTTCGGACCTTTCGGCGACGATGACCGCACCGTCATACGGCCCATGCCGGGCGGACGGCGCCC
GGCTCCGGCTCCGGCTCAGACGCCTCCCGTGCAACCCGCGCAGCCCGCCCCCGCGTATGCCGCGCCACCGCCGCCGCTAA
ATCCTGCACCCGCGCCCATGCCGGGATTCAATCCGTCCGCGCCGGTAGCCTTGCCGACCACCGAATTTCAGCAAAACCCC
ATGGTGGCCGCTGCATTATCAATGATTTCATTGGCCAGCCGTTTACGGCAAACCGCGGTTTATCCGGCGGTGGACGAACT
GCGTCAGCGTTTGTCGGCCGAAGTCAGCGGCTTCGAAAATAAATTATTGCAAACCGGCATCGGCGCCGATCAGGTGCGCA
TGGCCAGCTATGCGCTGTGCAGTTTTCTGGATGAAACCATACTCAATACGCCCTGGGGCTCGCAGAGCAATTGGGGCCAT
CAAAGTTTGTTGATTTCCTTTCACAAGGAAGCCTGGGGCGGCGAAAAATTTTTCGACATTATCGGCATGCTGGTCAAACA
ACCGGCTCAAAATCTGAATTTGATCGAACTGGCTTATTTATTTCTCAGTTTAGGTTTTCAGGGCAAGTTTCGGATTTTGT
CCAACGGCCTGAACGAACTGGAAAAATACCGGCTTGAGCTGTATCAGTTGATAGCGCGGGTGAAGGGCGATTACGAACGT
GAACTATCGCCGCGTTGGCAGGGTTTGCGCGACGTGCGTAATGCCTTGGTGCGCTATGTGCCGTTGTGGGTGGTCGGCGC
GGTATTGGGTGTGGCTTTGATTTTGACTTATATCGGCTTTGTGCTGGCTTTGAATGCCCGCTCGGACCAGCTTTTCGCCG
AATTATTCGGCTTGGGCAAGCAACCGATCGAAATCGCCCAGGCCGCCGCCGTCATTCCGTTACCGGCACCGGTGCCGAAA
CCCGTGGTGCCGGAACGCCGTGAACGCTTTAAGCAGTTACTGGCCAAGGAAATTGCCGCCGATATGGTCGAGGTCGTGGA
CGATAAATTATTACGCATACGTAATTCGTTTGCCTCCGGCAGCGACCAGCTCAAACCCCAGTTCGAACCCATGCTGAAAA
AAATCTCCGACGAACTGGTGGCGGGTAACGACCGGGTATTGGTCACGGGCCATACCGATAACGTGCCGATCCGCTCCGCG
CGCTTTCCGTCAAATTGGCATTTATCCACTGCCAGGGCCAAATCGGTTGCCGACGATATGTTGAAAGCAGCGCCGGCGCT
GCAAGGCAAAATCCGCTCCGAAGGCCGGGCGGACAGCGAAGGCCTGGTGCCTAACGATACTCCCGAACATCGCGCGATCA
ATCGCCGCGTCGATTTACTGATTCAGTGA