Detailed information of component protein


Summary

Component ID T6CP004623
Component protein type TssM
T6SS ID (Type) T6SS00444 insolico (Type Unknown)
Strain Methylomonas methanica MC09
Replicon chromosome
Sequence Protein sequence (1182 a.a.); Nucleotide sequence (3546 bp)
Reference
-
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) METME_RS17265
Coordinate (Strand) 3805083..3808628 (-)
NCBI ID WP_013820036.1
RefSeq NC_015572
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.6e-93 196..454
IcmF-related PF06761.14 1.1e-67 505..802
IcmF_C PF06744.14 6.2e-26 1057..1161
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MGKLKAFFTNKW
Transmembrane helix 13-35 VIQFLGLLAFSILIWLAGPLIAF
Outside 36-44 AGSAPLESE
Transmembrane helix 45-64 LVRILVIATVFVGWVIFRLI
Inside 65-448 MQIRAGNTEKQLVEELVANDADQVAMASEDEVDTLRKGFEEALTVLKQSRTEAKSGSQYIYQLPWYAIIGAPGSGKTTALINSGLHFPLAEKLGKHAIKGVSGTRNCDWWFADDAVFLDTAGRYTTQESHQAVDAAGWAGFLNLIKKHRPLRPLNGVIVATSISDLLQQSEQERVEHAKAIRNRINELYQQLGVRLPVYLLFTKADLVAGFSDFFADLAPEQRAQVWGETFSATTLQGDHDVVTQFGDAYAELLQRLQMRTLNRIQDERDMQRRGAILDFPQQMALLKPAMLDFLQATFAVNRYEQAVLLRGVYFTSGTQEGTPIDRVLGILASAFRLDRQAAPMYSGQGKSFFLTRLLKDVLFPEAELAGQDPKLAKRKRLMQ
Transmembrane helix 449-471 MAAYIGSGLLFLLVIGLWTASYF
Outside 472-1181 NNQSILDKVEAQIAEYHAIKSSGSDSQGNFNALLPRLNILLAIQEDYKDSGIMSRFGLSQADKIQAAAQHSYETILRNYFLPAIQMRLKERMQGPEANNLDVLYQLLKVYLMFNQTDKLDPATAVAWIRADWDRQYATDPESLGQLVLHLDNLLKLQLDPMQIDEGFVGAVRNKLTQVPLIGQIYSRFKTEALIDQSHDFKLGKALGPDTTRVFVLSDGKDVSAYTIPGLFTAYGYTELFLKKSRDFVKDAVEQNWVLGSQSKADVLQIQQLHGELKKLYLNEYQAAWSDLLAKLKLQSAVTTNQTAQILDILSRPDGPLHALLVSIDENTALTRLNKQVSDALANVAGKALAAAGAAGGQALALAQDAAGLDTGPDPVQMVEDKFEPLRNLVAGGPDKPSALDPVLQQLKSLRDYFLQLSTANTGGQALQNQANLFSGAGMDVLKQAQLEFARLPEPLKTWFQIIVNSGGNKLSSAAKAQLSDMVKTGVASPCKAALNGRYPFSSASSQDVLLADFAKLFAPSGLIDQFFQANLKTFVDTTKPVWTEMAAEKPLGLSQASIRQFQTASKIRDAFFAVGNMPQVQFELKPQLLDNNVGTFRLQVEGQEAVYRHGPEQSISMKWPGPNPSQGVRIVFETLDGRQVSRGKEGTWAFFRLLDEATIVQGNAPERFTLTFKLQGMSASYQLLAASVNNPFNLQELQSFRCPDAL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9837 - -


  Protein sequence: 1182 a.a.    

>T6CP004623 NC_015572:c3808628-3805083 [Methylomonas methanica MC09] [TssM]
MGKLKAFFTNKWVIQFLGLLAFSILIWLAGPLIAFAGSAPLESELVRILVIATVFVGWVIFRLIMQIRAGNTEKQLVEEL
VANDADQVAMASEDEVDTLRKGFEEALTVLKQSRTEAKSGSQYIYQLPWYAIIGAPGSGKTTALINSGLHFPLAEKLGKH
AIKGVSGTRNCDWWFADDAVFLDTAGRYTTQESHQAVDAAGWAGFLNLIKKHRPLRPLNGVIVATSISDLLQQSEQERVE
HAKAIRNRINELYQQLGVRLPVYLLFTKADLVAGFSDFFADLAPEQRAQVWGETFSATTLQGDHDVVTQFGDAYAELLQR
LQMRTLNRIQDERDMQRRGAILDFPQQMALLKPAMLDFLQATFAVNRYEQAVLLRGVYFTSGTQEGTPIDRVLGILASAF
RLDRQAAPMYSGQGKSFFLTRLLKDVLFPEAELAGQDPKLAKRKRLMQMAAYIGSGLLFLLVIGLWTASYFNNQSILDKV
EAQIAEYHAIKSSGSDSQGNFNALLPRLNILLAIQEDYKDSGIMSRFGLSQADKIQAAAQHSYETILRNYFLPAIQMRLK
ERMQGPEANNLDVLYQLLKVYLMFNQTDKLDPATAVAWIRADWDRQYATDPESLGQLVLHLDNLLKLQLDPMQIDEGFVG
AVRNKLTQVPLIGQIYSRFKTEALIDQSHDFKLGKALGPDTTRVFVLSDGKDVSAYTIPGLFTAYGYTELFLKKSRDFVK
DAVEQNWVLGSQSKADVLQIQQLHGELKKLYLNEYQAAWSDLLAKLKLQSAVTTNQTAQILDILSRPDGPLHALLVSIDE
NTALTRLNKQVSDALANVAGKALAAAGAAGGQALALAQDAAGLDTGPDPVQMVEDKFEPLRNLVAGGPDKPSALDPVLQQ
LKSLRDYFLQLSTANTGGQALQNQANLFSGAGMDVLKQAQLEFARLPEPLKTWFQIIVNSGGNKLSSAAKAQLSDMVKTG
VASPCKAALNGRYPFSSASSQDVLLADFAKLFAPSGLIDQFFQANLKTFVDTTKPVWTEMAAEKPLGLSQASIRQFQTAS
KIRDAFFAVGNMPQVQFELKPQLLDNNVGTFRLQVEGQEAVYRHGPEQSISMKWPGPNPSQGVRIVFETLDGRQVSRGKE
GTWAFFRLLDEATIVQGNAPERFTLTFKLQGMSASYQLLAASVNNPFNLQELQSFRCPDAL*

  Nucleotide sequence: 3546 bp    

>T6CP004623 NC_015572:c3808628-3805083 [Methylomonas methanica MC09] [TssM]
ATGGGCAAGCTCAAAGCATTTTTTACCAATAAATGGGTTATTCAGTTTCTGGGTTTGCTGGCGTTCAGTATCCTGATCTG
GCTGGCCGGGCCGTTGATAGCCTTCGCGGGCAGTGCGCCGCTGGAGTCGGAACTGGTCAGGATTTTGGTCATCGCCACCG
TGTTTGTCGGCTGGGTGATTTTCCGGCTGATCATGCAGATTCGGGCGGGTAATACCGAAAAACAACTGGTCGAGGAATTG
GTCGCCAACGATGCCGATCAGGTCGCCATGGCGTCCGAAGATGAAGTGGATACGCTACGCAAAGGCTTTGAGGAAGCATT
GACGGTGTTGAAGCAATCGCGCACCGAAGCAAAATCCGGCAGCCAGTATATTTATCAATTACCCTGGTATGCCATCATCG
GTGCGCCCGGTTCCGGTAAAACCACGGCCTTGATCAACTCCGGTTTGCATTTTCCACTGGCGGAAAAACTGGGCAAGCAT
GCGATAAAAGGCGTCAGCGGCACCCGAAATTGCGATTGGTGGTTTGCCGACGACGCGGTGTTTCTGGATACGGCCGGCCG
CTATACCACGCAGGAAAGCCATCAAGCCGTGGATGCGGCCGGATGGGCCGGCTTTTTGAATCTGATCAAAAAGCATCGGC
CGCTACGCCCCCTGAACGGAGTCATCGTCGCCACCAGTATTTCCGATCTGTTGCAGCAATCCGAGCAGGAACGGGTCGAA
CATGCCAAAGCCATTCGCAACCGCATCAACGAGTTGTATCAACAGCTGGGCGTGCGTTTGCCGGTGTATTTGCTGTTTAC
CAAAGCCGATTTGGTAGCCGGATTCAGCGACTTTTTCGCCGATTTAGCCCCGGAGCAGCGGGCTCAAGTGTGGGGGGAAA
CCTTTTCCGCCACCACCTTACAGGGCGATCACGATGTAGTGACTCAATTCGGCGACGCTTATGCGGAATTACTGCAACGT
TTGCAAATGCGCACGCTCAATCGTATTCAGGATGAACGCGATATGCAACGTCGGGGAGCCATCCTGGATTTTCCGCAGCA
GATGGCTTTATTAAAGCCGGCCATGCTGGATTTTTTGCAGGCAACCTTTGCGGTCAATCGCTACGAGCAAGCCGTGTTAT
TGCGGGGGGTGTATTTCACCAGCGGCACCCAGGAAGGCACGCCCATCGACCGGGTATTGGGTATTCTGGCCAGCGCCTTT
CGTCTGGATAGGCAAGCCGCGCCTATGTATAGCGGTCAAGGTAAAAGCTTTTTCCTGACCCGTTTGTTAAAGGATGTATT
GTTTCCGGAAGCGGAGCTGGCCGGACAAGATCCCAAATTGGCCAAACGCAAACGCTTGATGCAAATGGCCGCTTATATCG
GTTCCGGTTTGCTGTTTTTGCTGGTGATCGGTTTGTGGACCGCCAGTTATTTCAACAACCAATCGATACTGGATAAGGTG
GAGGCGCAAATCGCCGAATACCACGCCATCAAAAGCAGTGGCAGCGATAGCCAAGGCAATTTCAATGCCTTGTTGCCGCG
CTTGAATATTTTGCTGGCCATACAGGAAGATTACAAAGACAGTGGCATCATGTCCAGGTTCGGCCTATCGCAAGCCGATA
AAATTCAGGCAGCCGCCCAGCATAGTTACGAAACCATCCTGCGTAATTATTTTTTACCCGCGATTCAGATGCGTTTGAAA
GAACGCATGCAAGGACCCGAAGCCAATAATCTGGACGTGCTCTACCAGCTGTTAAAAGTCTATTTAATGTTCAACCAGAC
CGATAAACTCGATCCCGCTACCGCTGTGGCCTGGATCCGCGCGGATTGGGACAGACAATACGCGACCGACCCGGAAAGTC
TGGGGCAGTTGGTGCTGCATCTGGATAATCTGCTCAAGCTGCAGCTGGACCCGATGCAGATCGATGAAGGGTTTGTCGGC
GCGGTGCGTAACAAGCTCACGCAAGTGCCGCTGATCGGGCAGATTTACTCCCGCTTCAAAACCGAGGCCTTAATAGACCA
AAGCCACGATTTTAAGTTGGGTAAAGCATTGGGGCCGGATACCACTCGGGTATTCGTGTTGTCGGACGGCAAAGATGTGT
CGGCTTATACCATTCCGGGCTTGTTTACGGCCTATGGCTACACCGAGTTGTTTCTAAAAAAAAGCCGGGATTTCGTCAAG
GACGCTGTAGAACAGAATTGGGTGCTGGGTAGCCAGTCCAAAGCCGACGTTTTGCAAATTCAGCAATTACACGGCGAATT
GAAGAAACTGTATTTAAACGAATACCAGGCGGCTTGGTCTGATTTGCTGGCTAAATTGAAATTACAATCGGCGGTCACGA
CCAATCAGACCGCGCAAATCCTGGACATTTTGTCGAGGCCCGACGGTCCTCTGCACGCCTTGTTGGTCAGTATCGATGAA
AATACCGCGCTAACCCGCTTGAATAAGCAAGTGAGCGATGCACTGGCCAATGTTGCCGGAAAAGCGTTGGCTGCCGCAGG
TGCGGCAGGCGGCCAAGCGTTGGCGCTGGCGCAGGATGCCGCAGGCCTGGATACCGGCCCAGACCCGGTGCAGATGGTGG
AAGACAAGTTTGAACCCCTGCGCAATCTGGTAGCCGGCGGACCGGACAAGCCGAGCGCCTTGGACCCGGTATTGCAGCAA
TTGAAAAGTCTGCGCGATTACTTTCTGCAACTCAGCACTGCCAATACCGGCGGTCAGGCGTTACAAAATCAGGCCAATTT
ATTCAGCGGCGCCGGTATGGATGTACTGAAACAGGCGCAATTGGAGTTTGCCCGCTTGCCGGAACCCTTGAAAACCTGGT
TCCAGATTATCGTCAATAGCGGCGGCAATAAGCTGTCTTCCGCCGCCAAGGCTCAGTTGAGCGATATGGTGAAAACCGGG
GTGGCGTCGCCTTGCAAGGCGGCATTGAACGGCCGTTATCCGTTTTCCAGCGCTTCCTCGCAGGATGTGTTATTGGCCGA
TTTTGCCAAACTGTTTGCGCCGTCCGGTTTGATCGACCAGTTTTTTCAAGCCAATCTGAAAACCTTTGTCGACACCACCA
AGCCGGTGTGGACGGAAATGGCCGCCGAGAAACCGCTCGGTTTGTCGCAAGCCTCGATTCGTCAATTTCAAACTGCCTCC
AAAATACGCGATGCTTTTTTTGCTGTCGGCAACATGCCGCAAGTGCAATTCGAATTAAAGCCGCAATTGTTGGATAACAA
TGTCGGCACCTTTCGCTTGCAGGTGGAAGGGCAAGAGGCTGTGTATCGTCACGGTCCCGAACAATCCATCAGCATGAAAT
GGCCCGGCCCTAACCCCAGTCAGGGCGTGCGCATCGTGTTTGAAACGCTGGACGGACGTCAGGTCAGTCGCGGCAAGGAA
GGCACCTGGGCATTTTTTCGCTTGCTGGATGAAGCCACCATTGTGCAGGGCAACGCCCCGGAACGGTTTACCCTTACCTT
CAAATTGCAGGGTATGAGCGCCAGTTACCAACTGCTGGCCGCCAGCGTTAACAATCCCTTTAATTTACAGGAACTGCAAA
GTTTCCGTTGCCCCGATGCCTTGTGA