Detailed information of component protein


Summary

Component ID T6CP004622
Component protein type TssH
T6SS ID (Type) T6SS00443 experimental (Type i3)
Strain Methylomonas methanica MC09
Replicon chromosome
Sequence Protein sequence (907 a.a.); Nucleotide sequence (2721 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) METME_RS04340
Coordinate (Strand) 923804..926524 (+)
NCBI ID WP_013817564.1
RefSeq NC_015572
Uniprot ID G0A6J5_METMM
KEGG ID mmt:Metme_0858
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 1.2e-37 638..805
AAA_lid_9 PF17871.3 2.2e-28 373..465
ClpB_D2-small PF10431.11 2.8e-13 811..885
AAA PF00004.31 2.2e-09 234..368
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-906 MAEISRVKLFGKLNRVCYKAIEGATVFCKLRGNPYVELVHWLNQLLQLPDSDLHRIIKQYSLDGSRLAKDVTDALERLPRGSTAISDFSPHIEESVERGWVYGTLMFGESQVRTGYLLVGLVKTKSLRNELLGISKEFEKIKPDSLSDELAQIVSGSPEDGLAATDGFQSGATPGEASGAMAPAAMGKQEALKQFTVDLTEQARTGKIDPIVGRDEEIRQLIDILMRRRQNNPILTGEAGVGKTAVVEGFALKIVAGDVPPSLREVSLRTLDVGLLQAGASMKGEFENRLRQVIEEVQSSPKPIILFIDEAHTLVGAGGAAGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVLEPSEEKAIRMMRGVVSVQEKHHQVLILDEALEAAVKLSHRYIPARQLPDKAVSLLDTACARVGISQHAVPPAVDDCRKRIDALETELAIIGREKTVGIEVVGREQEATEKLEAEKVRLAELETRWETEKGLVEKILGLRAKLRAAGHAVDEATKPTDSSTADAAEQPASETAEDKDALLAELRALQTELHAFQGESPLILPTVDHQAVAAVVQDWTGIPVGRMVKNEIETVLKLADNLEQRIIGQRHALEMIARRIQTSRAGLDNPNKPIGVFMLAGTSGVGKTETALALAEALYGGEQNVITINMSEYQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEVEKAHPDVHEIFFQVFDKGWMEDGEGRVIDFKNTLILLTTNAGTDLIAGLCSDPELMPDPEGISKALREPLLKVFPPALLGRLVVIPYYPLNDEMIGAIARLQLGRIKKRIAESHKVPFSYDDEVVKLIASRCTELESGGRMIDAILTNTVLPKISEEFLVRMVEGKPIERVHVTVQDGEFGYGFE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9985 - -


  Protein sequence: 907 a.a.    

>T6CP004622 NC_015572:923804-926524 [Methylomonas methanica MC09] [TssH]
MAEISRVKLFGKLNRVCYKAIEGATVFCKLRGNPYVELVHWLNQLLQLPDSDLHRIIKQYSLDGSRLAKDVTDALERLPR
GSTAISDFSPHIEESVERGWVYGTLMFGESQVRTGYLLVGLVKTKSLRNELLGISKEFEKIKPDSLSDELAQIVSGSPED
GLAATDGFQSGATPGEASGAMAPAAMGKQEALKQFTVDLTEQARTGKIDPIVGRDEEIRQLIDILMRRRQNNPILTGEAG
VGKTAVVEGFALKIVAGDVPPSLREVSLRTLDVGLLQAGASMKGEFENRLRQVIEEVQSSPKPIILFIDEAHTLVGAGGA
AGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVLEPSEEKAIRMMRGVVSVQEKHHQVLILDEA
LEAAVKLSHRYIPARQLPDKAVSLLDTACARVGISQHAVPPAVDDCRKRIDALETELAIIGREKTVGIEVVGREQEATEK
LEAEKVRLAELETRWETEKGLVEKILGLRAKLRAAGHAVDEATKPTDSSTADAAEQPASETAEDKDALLAELRALQTELH
AFQGESPLILPTVDHQAVAAVVQDWTGIPVGRMVKNEIETVLKLADNLEQRIIGQRHALEMIARRIQTSRAGLDNPNKPI
GVFMLAGTSGVGKTETALALAEALYGGEQNVITINMSEYQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRRPYSVVLLDE
VEKAHPDVHEIFFQVFDKGWMEDGEGRVIDFKNTLILLTTNAGTDLIAGLCSDPELMPDPEGISKALREPLLKVFPPALL
GRLVVIPYYPLNDEMIGAIARLQLGRIKKRIAESHKVPFSYDDEVVKLIASRCTELESGGRMIDAILTNTVLPKISEEFL
VRMVEGKPIERVHVTVQDGEFGYGFE*

  Nucleotide sequence: 2721 bp    

>T6CP004622 NC_015572:923804-926524 [Methylomonas methanica MC09] [TssH]
ATGGCAGAAATCAGCCGAGTCAAACTGTTTGGAAAATTAAACCGGGTCTGCTACAAGGCAATCGAAGGGGCGACCGTGTT
TTGCAAACTCAGGGGAAATCCGTATGTTGAGTTGGTGCATTGGTTAAATCAACTGTTGCAATTGCCCGATTCTGATTTGC
ACCGCATCATCAAGCAATACAGTCTGGACGGTTCCCGCTTGGCCAAGGATGTGACCGATGCGCTGGAACGCTTGCCGCGC
GGTTCCACCGCTATTTCCGATTTCTCGCCGCATATCGAGGAATCGGTGGAACGAGGCTGGGTTTACGGTACCTTGATGTT
TGGCGAAAGCCAAGTGCGTACCGGCTACTTATTGGTGGGCTTGGTCAAAACCAAATCGTTGCGCAATGAACTGCTGGGTA
TTTCCAAGGAATTTGAAAAGATCAAACCCGACTCGTTGTCGGACGAATTGGCGCAGATTGTCTCCGGTTCGCCTGAAGAC
GGCTTGGCGGCAACAGACGGTTTTCAATCCGGCGCCACGCCCGGCGAAGCCAGCGGCGCCATGGCGCCTGCGGCCATGGG
TAAACAGGAAGCCCTGAAACAATTCACCGTCGATCTGACCGAACAAGCCAGAACCGGCAAAATCGATCCCATCGTCGGCC
GCGATGAAGAAATACGCCAACTGATCGATATTCTGATGCGCCGCCGTCAAAACAACCCGATACTCACCGGGGAAGCCGGG
GTTGGTAAAACCGCCGTGGTGGAAGGCTTTGCCTTAAAAATTGTGGCCGGCGATGTGCCGCCGTCGCTGCGGGAAGTCAG
TTTGCGCACACTGGACGTCGGTTTGTTGCAAGCCGGGGCCAGCATGAAGGGTGAGTTCGAGAATCGCTTGCGGCAAGTGA
TTGAAGAAGTGCAATCGTCACCGAAACCGATCATTCTATTTATCGACGAAGCGCATACCCTGGTCGGTGCCGGCGGCGCT
GCAGGTACCGGCGACGCCGCCAATTTATTGAAACCGGCCTTGGCGCGCGGCACACTCAGAACCGTTGCCGCGACCACTTG
GGCCGAATACAAAAAACATATCGAAAAAGATCCGGCCCTGACGCGCCGTTTTCAAGTTGTGCAGGTGCTGGAGCCTAGCG
AAGAAAAAGCCATCCGTATGATGCGCGGGGTAGTGTCGGTACAGGAAAAACACCATCAGGTATTGATTCTGGACGAAGCA
TTGGAAGCGGCTGTAAAACTGTCGCATCGCTATATTCCAGCCAGACAATTACCGGATAAAGCCGTCAGCTTGCTGGATAC
AGCCTGCGCGCGGGTAGGCATCAGCCAGCATGCCGTGCCGCCGGCCGTAGACGATTGCCGCAAACGCATCGATGCTCTGG
AGACCGAGCTGGCCATCATCGGTAGGGAAAAAACCGTGGGGATCGAAGTAGTCGGCCGCGAACAGGAAGCCACCGAAAAG
CTTGAAGCCGAAAAAGTTCGCCTGGCGGAACTGGAAACCCGTTGGGAAACCGAAAAAGGTTTGGTAGAGAAAATTCTGGG
ATTACGCGCCAAGCTCAGAGCTGCGGGGCATGCCGTCGACGAAGCAACCAAGCCAACCGATTCTTCGACTGCGGATGCGG
CCGAGCAACCCGCGAGTGAGACGGCAGAAGATAAGGACGCTTTGCTCGCCGAACTCAGGGCATTACAAACTGAACTGCAT
GCGTTCCAGGGCGAATCGCCGTTGATTTTACCGACCGTGGATCATCAGGCCGTCGCGGCGGTAGTGCAGGACTGGACCGG
TATTCCGGTCGGCCGCATGGTGAAAAACGAAATCGAAACCGTGCTGAAACTGGCCGACAATCTGGAACAGCGTATCATTG
GCCAACGTCATGCCCTGGAAATGATCGCCCGTCGAATTCAAACTTCCCGCGCGGGGCTCGACAACCCCAACAAACCGATC
GGCGTGTTCATGCTGGCCGGTACGTCCGGGGTGGGTAAAACCGAAACTGCCTTGGCATTGGCCGAAGCCCTATACGGCGG
TGAACAAAACGTTATCACCATCAATATGAGCGAGTATCAGGAAGCGCATACCGTTTCCACCTTGAAAGGCGCGCCTCCAG
GGTATGTCGGCTACGGCGAAGGCGGGGTGCTGACAGAAGCCGTGCGTCGTCGTCCCTATAGCGTAGTGCTGCTGGACGAG
GTGGAAAAAGCCCATCCGGACGTGCATGAGATATTCTTTCAGGTGTTCGACAAAGGCTGGATGGAAGACGGCGAAGGCCG
GGTCATCGATTTTAAAAATACTCTGATTTTGTTGACCACCAACGCCGGTACCGATTTGATAGCCGGCCTTTGCAGCGACC
CTGAATTGATGCCGGATCCCGAAGGTATTTCCAAAGCCTTGCGGGAACCGTTACTCAAAGTGTTCCCGCCTGCATTGCTG
GGCCGTTTGGTGGTGATTCCTTATTATCCGCTCAACGACGAAATGATAGGCGCCATCGCCCGTCTGCAGCTGGGTCGTAT
CAAAAAACGCATTGCCGAAAGCCACAAAGTGCCGTTCAGCTACGACGACGAAGTCGTCAAACTTATCGCCAGCCGTTGCA
CCGAATTGGAAAGCGGCGGCCGCATGATAGATGCGATTTTGACCAATACCGTGTTGCCGAAAATCAGCGAGGAGTTTTTG
GTCCGCATGGTGGAAGGCAAGCCGATCGAGCGGGTGCATGTCACCGTGCAGGATGGGGAGTTTGGATATGGGTTCGAGTG
A