Detailed information of component protein


Summary

Component ID T6CP004620
Component protein type TssF
T6SS ID (Type) T6SS00443 experimental (Type i3)
Strain Methylomonas methanica MC09
Replicon chromosome
Sequence Protein sequence (628 a.a.); Nucleotide sequence (1884 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) METME_RS04330
Coordinate (Strand) 920853..922736 (+)
NCBI ID WP_013817562.1
RefSeq NC_015572
Uniprot ID G0A6J3_METMM
KEGG ID mmt:Metme_0856
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 9.9e-238 1..624
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-627 MDPRLLHYYNRELQHVREVGAEFASEFPKIAARLGLDAFECSDPYVERLFEGFAFMAARVQLKVDAEFPNFTQHLLEIVYPHYLAATPSMAVVEFKPDLTEESLAEGFLIDKGSALRSQIAKGEQTACEYQTAHPLQLWPLEIGQVEYLPSLAAVSSCGLADPTHLQHVKGAIRIVLRCTAGVRFNQLSLDTLPLFLRGAGAIPYRLYEQLLANSKALACKFNNGSQVQVKFAGGKHLRGVGFEKDEAMLRYTPRSFDGYRILQEYFAFPERFLFAELSGLSSLIAECQTDELELFVLLDRSDAQLINALDKANLALFCVPAINLFSKRTDRLHVDRRQSEYHVLVDRTRPMDYEVFSVEHVAGFGEDRNEQAFLPFYGSKSVYQNQSETAYYMLRRQKRILSSKQRRKGMRSSYIGSECFISIVDTEQAPYSPQINQLGLETLCTNRDLPMVMPVGLGDTDFSLQISAPVKSIRCISGPTRPLPAAYAGESVWKLINHLSLNYLSLIDSDPKQGAAALRELLSLYAENREPTIKKQIEGVISVNAKNVVRRIDSKGPMVFGRGLEITVLLDESAFEGGGYFMMGTVLDQFFTRYVSINSFIETVIRTTDRGEVARWPTRIGRRHTF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9934 - -


  Protein sequence: 628 a.a.    

>T6CP004620 NC_015572:920853-922736 [Methylomonas methanica MC09] [TssF]
MDPRLLHYYNRELQHVREVGAEFASEFPKIAARLGLDAFECSDPYVERLFEGFAFMAARVQLKVDAEFPNFTQHLLEIVY
PHYLAATPSMAVVEFKPDLTEESLAEGFLIDKGSALRSQIAKGEQTACEYQTAHPLQLWPLEIGQVEYLPSLAAVSSCGL
ADPTHLQHVKGAIRIVLRCTAGVRFNQLSLDTLPLFLRGAGAIPYRLYEQLLANSKALACKFNNGSQVQVKFAGGKHLRG
VGFEKDEAMLRYTPRSFDGYRILQEYFAFPERFLFAELSGLSSLIAECQTDELELFVLLDRSDAQLINALDKANLALFCV
PAINLFSKRTDRLHVDRRQSEYHVLVDRTRPMDYEVFSVEHVAGFGEDRNEQAFLPFYGSKSVYQNQSETAYYMLRRQKR
ILSSKQRRKGMRSSYIGSECFISIVDTEQAPYSPQINQLGLETLCTNRDLPMVMPVGLGDTDFSLQISAPVKSIRCISGP
TRPLPAAYAGESVWKLINHLSLNYLSLIDSDPKQGAAALRELLSLYAENREPTIKKQIEGVISVNAKNVVRRIDSKGPMV
FGRGLEITVLLDESAFEGGGYFMMGTVLDQFFTRYVSINSFIETVIRTTDRGEVARWPTRIGRRHTF*

  Nucleotide sequence: 1884 bp    

>T6CP004620 NC_015572:920853-922736 [Methylomonas methanica MC09] [TssF]
ATGGATCCTCGCCTGCTTCATTACTATAACCGCGAATTGCAGCATGTACGGGAAGTTGGGGCTGAGTTCGCCAGCGAATT
CCCTAAAATTGCCGCTCGTTTAGGTCTGGATGCCTTCGAGTGTTCCGATCCTTACGTAGAACGTTTATTCGAAGGGTTTG
CTTTTATGGCAGCCCGGGTGCAACTGAAGGTCGATGCCGAATTTCCCAACTTTACCCAGCATCTGTTGGAGATTGTCTAC
CCGCATTATCTGGCCGCTACACCATCAATGGCCGTGGTGGAATTTAAGCCGGATTTGACGGAAGAAAGTCTGGCGGAAGG
TTTTTTGATCGATAAAGGTAGTGCTTTACGCAGCCAAATCGCCAAAGGTGAGCAAACCGCATGCGAATACCAGACCGCGC
ATCCCTTGCAACTGTGGCCGTTGGAAATCGGTCAGGTGGAATACCTACCGTCTTTAGCCGCGGTTTCCAGTTGCGGTTTG
GCCGACCCCACACATTTGCAACATGTTAAAGGCGCTATCCGCATTGTCCTGCGTTGCACGGCCGGGGTACGATTTAATCA
GCTATCGCTGGACACCTTGCCGCTGTTTTTACGTGGCGCGGGGGCGATTCCTTACCGCTTATATGAACAATTATTGGCCA
ATTCGAAAGCCTTGGCCTGTAAATTTAACAATGGCTCACAAGTCCAGGTTAAATTTGCCGGCGGCAAACATCTGCGTGGC
GTGGGCTTTGAAAAAGACGAAGCCATGTTGCGATATACGCCGAGGTCTTTCGATGGCTATCGAATTCTGCAGGAATATTT
TGCCTTTCCCGAGCGCTTTCTGTTTGCCGAATTATCCGGCCTTTCGTCATTAATTGCCGAGTGCCAAACCGATGAACTGG
AGTTGTTCGTCTTATTGGACCGCAGCGATGCGCAACTGATCAATGCGTTGGATAAAGCCAATCTGGCCTTGTTCTGCGTG
CCGGCCATAAACCTGTTTTCTAAGCGCACCGACCGCTTGCACGTGGATCGCCGCCAGTCGGAATACCATGTGCTGGTGGA
CCGCACGCGACCGATGGATTACGAAGTTTTCAGCGTCGAGCATGTGGCGGGTTTCGGCGAAGACCGTAACGAACAGGCCT
TTTTACCGTTTTATGGCTCGAAAAGCGTTTATCAGAATCAATCCGAAACCGCCTATTACATGTTGCGGCGGCAAAAACGT
ATATTGTCATCCAAACAACGCCGCAAGGGCATGCGATCCAGCTATATCGGCAGCGAATGTTTTATTTCCATAGTCGATAC
CGAACAAGCGCCTTATTCGCCGCAAATCAATCAATTGGGTTTGGAAACACTTTGTACCAACCGTGACTTGCCGATGGTGA
TGCCGGTAGGTTTGGGCGATACCGATTTTAGTCTGCAAATCAGCGCGCCGGTTAAGTCGATTCGCTGTATTTCCGGCCCC
ACCCGGCCTTTACCGGCGGCGTATGCCGGCGAGAGTGTTTGGAAACTTATAAACCATCTCTCGTTGAATTACCTATCGCT
GATCGACAGCGATCCTAAGCAAGGGGCTGCGGCCTTGCGCGAATTATTAAGTCTGTATGCCGAAAATCGCGAGCCGACTA
TCAAAAAGCAAATCGAAGGGGTTATCTCCGTTAATGCCAAGAATGTGGTCAGGCGCATCGATTCCAAAGGGCCCATGGTA
TTTGGACGCGGTCTGGAGATTACGGTGTTGTTGGATGAATCTGCATTTGAGGGGGGCGGCTATTTCATGATGGGTACGGT
TTTGGATCAGTTTTTTACCCGCTACGTATCCATTAATTCCTTTATCGAAACCGTCATCCGTACCACGGATCGCGGAGAGG
TGGCACGATGGCCGACCAGAATCGGACGCCGGCACACGTTTTAA