Detailed information of component protein
Summary
| Component ID | T6CP004616 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS00443  (Type i3) |
| Strain | Methylomonas methanica MC09 |
| Replicon | chromosome |
| Sequence | Protein sequence (179 a.a.); Nucleotide sequence (537 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | METME_RS04305 |
| Coordinate (Strand) | 916832..917368 (+) |
| NCBI ID | WP_013817557.1 |
| RefSeq | NC_015572 |
| Uniprot ID | G0A6I8_METMM |
| KEGG ID | mmt:Metme_0851 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 1.7e-55 | 10..161 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-178 | MAESSQKFIQRNRAPRVQIEYDVEVYGSEKKVQLPFVMGVLSDLSGKPSEPLPPIADRKLLEIDVDNFNERLKSMKPRTAFQVANTLTGEGNLNVDITFEDMDDFSPAAVAKKVAGLDKIVEARTQLSNLITYMDGKTGAEELIAKLINDPALLQTLAEKAKPAEAEKPADSAEGSGE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9961 | - | - |
Protein sequence: 179 a.a.
>T6CP004616 NC_015572:916832-917368 [Methylomonas methanica MC09] [TssB]
MAESSQKFIQRNRAPRVQIEYDVEVYGSEKKVQLPFVMGVLSDLSGKPSEPLPPIADRKLLEIDVDNFNERLKSMKPRTA
FQVANTLTGEGNLNVDITFEDMDDFSPAAVAKKVAGLDKIVEARTQLSNLITYMDGKTGAEELIAKLINDPALLQTLAEK
AKPAEAEKPADSAEGSGE*
MAESSQKFIQRNRAPRVQIEYDVEVYGSEKKVQLPFVMGVLSDLSGKPSEPLPPIADRKLLEIDVDNFNERLKSMKPRTA
FQVANTLTGEGNLNVDITFEDMDDFSPAAVAKKVAGLDKIVEARTQLSNLITYMDGKTGAEELIAKLINDPALLQTLAEK
AKPAEAEKPADSAEGSGE*
Nucleotide sequence: 537 bp
>T6CP004616 NC_015572:916832-917368 [Methylomonas methanica MC09] [TssB]
ATGGCCGAAAGTAGTCAGAAGTTCATTCAACGAAACCGGGCGCCGCGCGTTCAAATCGAATACGATGTGGAAGTTTACGG
CTCCGAGAAAAAAGTACAACTTCCCTTTGTGATGGGTGTGCTTTCCGATCTCTCGGGTAAACCTTCCGAACCGTTACCTC
CCATTGCCGACCGGAAGTTATTGGAAATAGATGTCGATAACTTCAACGAGCGTTTGAAATCCATGAAACCCAGAACGGCA
TTTCAAGTGGCTAATACCCTCACCGGGGAAGGCAACCTGAATGTGGATATCACTTTCGAAGACATGGATGATTTTTCTCC
CGCAGCGGTAGCCAAAAAGGTGGCTGGTCTGGATAAAATTGTGGAAGCCAGAACCCAGCTGTCCAACCTGATTACCTATA
TGGACGGAAAAACCGGCGCCGAAGAACTGATTGCCAAATTGATCAATGATCCCGCCTTGTTGCAAACGTTGGCCGAAAAA
GCCAAACCTGCCGAAGCGGAAAAACCAGCGGATTCTGCTGAAGGAAGTGGGGAGTAA
ATGGCCGAAAGTAGTCAGAAGTTCATTCAACGAAACCGGGCGCCGCGCGTTCAAATCGAATACGATGTGGAAGTTTACGG
CTCCGAGAAAAAAGTACAACTTCCCTTTGTGATGGGTGTGCTTTCCGATCTCTCGGGTAAACCTTCCGAACCGTTACCTC
CCATTGCCGACCGGAAGTTATTGGAAATAGATGTCGATAACTTCAACGAGCGTTTGAAATCCATGAAACCCAGAACGGCA
TTTCAAGTGGCTAATACCCTCACCGGGGAAGGCAACCTGAATGTGGATATCACTTTCGAAGACATGGATGATTTTTCTCC
CGCAGCGGTAGCCAAAAAGGTGGCTGGTCTGGATAAAATTGTGGAAGCCAGAACCCAGCTGTCCAACCTGATTACCTATA
TGGACGGAAAAACCGGCGCCGAAGAACTGATTGCCAAATTGATCAATGATCCCGCCTTGTTGCAAACGTTGGCCGAAAAA
GCCAAACCTGCCGAAGCGGAAAAACCAGCGGATTCTGCTGAAGGAAGTGGGGAGTAA