SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP004493
Component protein type	TssH
T6SS ID (Type)	T6SS00432 (Type i3)
Strain	Pseudomonas brassicacearum subsp. brassicacearum NFM421
Replicon	chromosome
Sequence	Protein sequence (897 a.a.); Nucleotide sequence (2691 bp)
Reference	[1] Ortet P, Barakat M, Lalaouna D, et al. Complete genome sequence of a beneficial plant root-associated bacterium, Pseudomonas brassicacearum. J Bacteriol. 2011 Jun;193(12):3146. doi: 10.1128/JB.00411-11. Epub 2011 Apr 22. PMID: 21515771 [2] Lalaouna D, Fochesato S, Sanchez L, et al. Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs. Appl Environ Microbiol. 2012 Mar;78(6):1658-65. doi: 10.1128/AEM.06769-11. Epub 2012 Jan 13. PMID: 22247157

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSEBR_RS29760
Coordinate (Strand)	6709281..6711971 (+)
NCBI ID	WP_003206940.1
RefSeq	NC_015379
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	2.4e-38	625..791
AAA_lid_9	PF17871.3	3e-28	373..465
ClpB_D2-small	PF10431.11	9e-14	798..872
AAA	PF00004.31	5.4e-09	234..367

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 897 a.a.

						>T6CP004493 NC_015379:6709281-6711971 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssH]
MGEISRAALFGKLNSVAYKAIEAATVFCKLRGNPYVELAHWFHQLLQLQDSDLHRIIRQFNIEPARLARDLTEALDRLPR
GSTSITDLSSHVEEAVERGWVYGSLMFGESQVRTGYLVLGILKTPSLRHALLGLSSEFDKVKVEALSERFDEYVGDSPEN
ALTASDGFNAGAVPGEASGAMAPSAMGKQEALKRFTVDLTEQARSGKLDPIVGRDEEIRQLVDILMRRRQNNPILTGEAG
VGKTAVVEGFALRIVAGDVPPALKDVELRSLDVGLLQAGASMKGEFEQRLRQVIEDVQASPKPIILFIDEAHTLVGAGGA
AGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVAEPSEDKALLMMRGVASTMEKHHQVQILDEA
LEASVKLSHRYIPARQLPDKSVSLLDTACARVAISLHAVPAEVDDSRRRIEALETELQIIAREHAIGVVIGNRQTQSETL
LSAERERLAELEGRWAEEKTLVDELLATRATLRERVGVVDSEDNSEASDNESHQLREKLVDLQQRLTALQGETPLILPTV
DYQAVASVVADWTGIPVGRMARNELETVLNLDQHLKKRIIGQDHALQMIAKRIQTSRAGLDNPSKPIGVFMLAGTSGVGK
TETALALAEAMYGGEQNVITINMSEFQEAHTVSTLKGAPPGYIGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVHEIFF
QVFDKGVMEDGEGRVIDFKNTLILLTTNAGTELIAQVCKDPQNVPEPEEIAKALRQPLLEIFPPALLGRLVTIPYYPLSD
EMLKAITRLQLNRIKKRVESTHKVAFDYDDAVIDLIVSRCTETESGGRMIDTILTNSLLPDMSREFLTRMLEGKALAGVR
ISAVDNELQYDFSDAA*					

Nucleotide sequence: 2691 bp

						>T6CP004493 NC_015379:6709281-6711971 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssH]
ATGGGTGAAATCAGTCGCGCCGCGTTGTTCGGCAAACTCAACAGCGTGGCCTACAAAGCCATCGAAGCCGCCACCGTGTT
CTGCAAGTTGCGCGGTAACCCTTATGTGGAACTGGCCCACTGGTTTCACCAGTTGCTGCAACTGCAGGACTCGGACCTGC
ACCGCATCATCCGCCAGTTCAACATCGAACCGGCGCGCCTGGCCCGTGACCTGACCGAAGCCCTGGATCGCCTGCCACGG
GGTTCGACGTCCATCACCGACCTGTCGTCCCACGTCGAAGAAGCCGTGGAGCGCGGCTGGGTCTACGGCAGCCTGATGTT
CGGCGAAAGCCAGGTTCGCACCGGTTACCTGGTGCTGGGCATTCTCAAGACCCCGAGCCTGCGCCACGCACTGCTGGGCT
TGTCGTCGGAGTTCGATAAGGTCAAGGTCGAAGCCCTGAGCGAACGGTTTGACGAATACGTCGGCGACTCGCCGGAAAAC
GCCCTGACCGCCAGCGACGGCTTCAATGCCGGCGCGGTGCCGGGCGAAGCCAGCGGCGCCATGGCCCCTAGCGCCATGGG
CAAGCAGGAAGCCCTCAAGCGCTTTACCGTCGACCTCACCGAGCAGGCCCGCAGCGGCAAGCTCGACCCGATCGTCGGCC
GTGACGAAGAGATCCGCCAACTGGTGGACATCCTCATGCGCCGCCGGCAGAACAACCCGATCCTTACCGGTGAAGCCGGC
GTGGGCAAGACCGCCGTGGTCGAAGGCTTCGCCCTGCGCATCGTCGCCGGCGACGTGCCGCCGGCCCTCAAGGACGTGGA
ACTGCGCAGCCTCGACGTGGGCCTGTTGCAGGCCGGCGCCAGCATGAAAGGCGAGTTCGAACAGCGCCTGCGCCAGGTCA
TCGAAGACGTCCAGGCCTCGCCCAAGCCGATCATCCTGTTCATCGACGAAGCCCACACCCTGGTCGGTGCCGGTGGCGCC
GCCGGCACGGGCGACGCGGCCAACCTGCTCAAGCCGGCGTTGGCCCGTGGCACCTTGCGCACCGTGGCCGCCACGACCTG
GGCCGAGTACAAGAAGCACATCGAAAAAGACCCGGCCCTGACCCGCCGCTTCCAAGTGGTGCAAGTGGCCGAGCCGTCGG
AAGACAAGGCCCTGCTGATGATGCGCGGCGTGGCCTCGACCATGGAAAAACACCACCAGGTGCAGATCCTCGACGAAGCC
CTGGAAGCTTCGGTCAAGCTGTCCCACCGCTACATTCCCGCGCGCCAGCTGCCGGACAAATCCGTGAGCCTGCTGGACAC
CGCCTGCGCCCGCGTCGCCATCAGCCTGCACGCCGTACCGGCCGAAGTGGACGACAGCCGTCGGCGCATCGAAGCGCTGG
AAACCGAGTTGCAAATCATCGCCCGTGAACACGCGATTGGCGTGGTCATCGGCAACCGCCAGACCCAGAGCGAAACCCTG
CTGAGCGCCGAGCGCGAACGCCTGGCCGAGCTGGAAGGCCGCTGGGCCGAAGAGAAAACCCTGGTGGACGAACTGCTCGC
CACCCGCGCCACCTTGCGCGAACGCGTTGGCGTGGTGGACAGCGAAGACAACAGCGAAGCTTCTGACAACGAAAGCCACC
AACTGCGCGAGAAGCTGGTGGACCTGCAACAGCGCCTGACCGCCCTGCAAGGCGAAACCCCGCTGATCCTGCCGACCGTG
GATTACCAGGCCGTGGCCTCGGTGGTCGCCGACTGGACCGGTATCCCGGTGGGCCGCATGGCCCGCAACGAACTGGAAAC
CGTGCTCAACCTCGACCAGCACCTGAAGAAACGCATCATCGGCCAGGACCATGCCTTGCAGATGATTGCCAAGCGCATCC
AGACCTCCCGCGCCGGCCTCGACAACCCGAGCAAGCCGATTGGTGTGTTCATGCTCGCCGGCACCTCCGGCGTGGGCAAG
ACCGAAACCGCCCTGGCCCTGGCCGAAGCCATGTACGGCGGCGAGCAGAACGTCATCACCATCAACATGAGCGAGTTCCA
GGAAGCCCACACCGTATCGACCCTCAAGGGTGCGCCACCGGGCTACATCGGCTATGGCGAAGGCGGCGTGCTGACCGAAG
CCGTGCGGCGCAAACCGTACAGTGTGGTGTTGCTGGACGAGGTCGAGAAGGCCCACCCGGACGTGCATGAGATCTTCTTC
CAGGTGTTCGACAAAGGCGTGATGGAGGACGGTGAAGGCCGGGTGATCGACTTCAAGAACACCTTGATCCTGCTGACCAC
CAACGCCGGCACCGAGTTGATTGCCCAGGTCTGCAAAGACCCGCAGAACGTGCCCGAGCCGGAAGAAATCGCCAAGGCCC
TGCGCCAACCGCTGCTGGAGATTTTCCCGCCAGCCTTGCTGGGCCGCCTGGTGACGATCCCGTACTACCCGCTCAGCGAC
GAGATGCTCAAGGCCATCACCCGCCTGCAACTCAACCGCATCAAGAAGCGCGTGGAAAGCACCCACAAAGTCGCCTTCGA
CTACGACGACGCGGTGATCGACCTGATCGTCTCGCGTTGCACCGAAACCGAAAGCGGCGGGCGCATGATCGACACCATCC
TGACCAACAGCCTGCTGCCGGACATGAGCCGCGAGTTCCTGACCCGCATGCTCGAAGGCAAGGCTTTGGCCGGGGTGCGG
ATCAGCGCCGTGGACAATGAATTGCAGTACGATTTCAGCGACGCGGCCTGA					