SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP004480
Component protein type	TssH
T6SS ID (Type)	T6SS00431 (Type i4a)
Strain	Pseudomonas brassicacearum subsp. brassicacearum NFM421
Replicon	chromosome
Sequence	Protein sequence (848 a.a.); Nucleotide sequence (2544 bp)
Reference	[1] Ortet P, Barakat M, Lalaouna D, et al. Complete genome sequence of a beneficial plant root-associated bacterium, Pseudomonas brassicacearum. J Bacteriol. 2011 Jun;193(12):3146. doi: 10.1128/JB.00411-11. Epub 2011 Apr 22. PMID: 21515771 [2] Lalaouna D, Fochesato S, Sanchez L, et al. Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs. Appl Environ Microbiol. 2012 Mar;78(6):1658-65. doi: 10.1128/AEM.06769-11. Epub 2012 Jan 13. PMID: 22247157

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSEBR_RS17655
Coordinate (Strand)	4106625..4109168 (+)
NCBI ID	WP_013693579.1
RefSeq	NC_015379
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	1.7e-35	609..773
AAA_lid_9	PF17871.3	1.1e-29	360..451
Clp_N	PF02861.22	4.5e-10	21..72
AAA	PF00004.31	1.1e-09	218..323

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 848 a.a.

						>T6CP004480 NC_015379:4106625-4109168 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssH]
MELASLIGRLNPDNRRALERAAQRCMQRSHHYVEIEHLLLELLDIEGGDFACLLPRFGLERDAVATETNKALDLFKSGST
RTPALSAQTIGLLEDAVVQASVLGLDSIRSGLLLLALLDRDERRSLLLNSASSLLRIPRDALRAHLLEWTESSREHAGGV
RPAKPGEVPQKQDPVLDQYTQDLTADARAGRIDPIVGRDGEIRQCIDILLRRRQNNPILVGAPGVGKTAVVEGLALRIAA
GDVPPSLQEVTLRVLDLGLLQAGAGVKGEFEQRLKGVIDAVRSAEKPIILFIDEAHTLIGAGGAEGGSDAANLLKPALAR
GELRTLAATTWLEYKKYFEKDPALARRFQLVQVEEPDELTAVEMLRGVASKLEQHHGVQVLDAAIHEAVKLSHRYISGRQ
LPDKAISVLDTACARVALGQHDVPPPLESLRHRQNSLKDEVERLRREQATGLDHRERITLLEDESTSNVQAIRELETRWG
EEREAVRELLDTRRELLALSERADSEKADTEIDNRIDHLAAELLRLEAGLDAIRQDDPLVPEQVDSKTVAAVIAGWTGIP
VGKMLADEAHAVRTLGQRMGQRVMGQSTALNTIAQRLQAYRAGLTDPQKPVGVFLLVGPTGVGKTETAYALADALYGGER
NLISINLSEYQEAHTVSQLKGAPPGYVGYGSGGVLTEAVRRKPYSVVLLDEIEKAHPDVLEAFYNVFDKGLMEDGTGLVV
DFKNTVMLATSNVGAELLLDTPAAQLGSDAFNEALHKVLLQAFRPAFLARMTVVAYRPLDEATLEGIVLAKLEKLRGRYK
AATGKQFEFDAGIVKAVLAKCSAAGARDVENVLMTQVTGKLAEWVLE*					

Nucleotide sequence: 2544 bp

						>T6CP004480 NC_015379:4106625-4109168 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssH]
ATGGAACTGGCCAGCCTGATCGGACGCCTCAACCCGGACAACCGCCGCGCCCTGGAACGGGCCGCGCAACGTTGCATGCA
GCGCAGCCATCATTACGTGGAAATCGAACACCTGCTGTTGGAGTTGCTCGACATCGAGGGCGGTGATTTTGCCTGCCTGC
TGCCACGATTCGGTCTGGAGCGTGACGCGGTGGCGACGGAAACCAACAAGGCCCTGGACCTGTTCAAATCCGGCAGCACC
CGCACACCCGCCCTGTCGGCACAAACCATCGGCCTGCTGGAAGACGCCGTGGTCCAGGCCAGCGTGCTGGGCCTGGACAG
CATCCGCTCCGGGCTGTTGCTGCTGGCGCTGCTGGACCGCGATGAGCGCCGCAGCCTGCTGCTCAACAGCGCCTCGTCGT
TGCTGCGCATTCCTCGCGACGCCCTGCGCGCCCACCTATTGGAATGGACCGAAAGCTCCCGCGAGCATGCCGGTGGCGTG
CGCCCGGCCAAACCCGGCGAGGTGCCACAGAAACAGGACCCGGTGCTGGACCAGTACACCCAGGACCTGACCGCCGACGC
CCGGGCCGGGCGCATCGACCCCATCGTCGGCCGCGACGGCGAGATCCGCCAGTGCATCGACATTCTGCTCCGGCGCCGGC
AGAACAACCCGATCCTGGTGGGCGCACCAGGCGTCGGCAAGACCGCAGTGGTGGAAGGCCTGGCCCTGCGTATCGCCGCC
GGGGATGTGCCGCCGTCGTTGCAGGAAGTCACCCTGCGTGTCCTCGACCTGGGCCTGTTGCAGGCCGGTGCCGGTGTCAA
AGGTGAATTCGAACAACGCCTCAAAGGCGTGATCGACGCGGTACGCAGTGCCGAAAAACCGATCATCCTGTTCATCGACG
AAGCCCATACGCTGATCGGTGCCGGCGGCGCGGAAGGCGGCAGCGACGCGGCCAACCTGCTCAAGCCGGCCCTGGCCCGG
GGCGAACTGCGCACCCTGGCCGCCACCACCTGGCTGGAGTACAAAAAATATTTCGAGAAAGACCCGGCCCTGGCCCGCCG
CTTCCAACTGGTGCAGGTCGAAGAACCGGACGAACTTACCGCGGTGGAAATGCTCCGCGGCGTGGCGAGCAAACTGGAAC
AGCACCACGGCGTGCAGGTGCTGGACGCGGCCATCCACGAGGCGGTGAAGCTGTCCCACCGCTACATCTCCGGGCGACAG
TTGCCGGACAAAGCCATCAGCGTGCTCGACACCGCCTGCGCCCGGGTCGCCCTCGGCCAGCACGACGTGCCGCCGCCGTT
GGAAAGCCTGCGCCATCGGCAGAACAGCCTCAAGGACGAGGTCGAACGCCTGCGCCGGGAACAGGCCACCGGGCTCGATC
ACCGCGAGCGCATCACTCTGCTCGAAGACGAATCGACCAGCAATGTGCAGGCCATCCGCGAGCTGGAAACTCGCTGGGGC
GAAGAGCGCGAAGCCGTGCGCGAACTGCTCGACACCCGGCGCGAACTGCTTGCCCTGAGCGAACGCGCCGACAGTGAAAA
AGCCGACACCGAGATCGATAACCGCATCGACCACCTGGCCGCCGAACTGCTGCGCCTGGAAGCTGGCCTGGATGCCATTC
GCCAGGACGATCCGCTGGTGCCCGAACAGGTGGACAGCAAGACCGTCGCCGCCGTGATCGCCGGCTGGACCGGCATCCCG
GTGGGCAAGATGCTCGCCGACGAAGCCCACGCCGTGCGCACCCTCGGCCAGCGCATGGGCCAGCGGGTCATGGGCCAGAG
CACCGCGTTGAACACCATCGCCCAACGCCTGCAAGCCTACCGCGCCGGCCTCACCGACCCACAGAAACCGGTCGGCGTAT
TCCTGCTGGTGGGCCCCACCGGCGTGGGCAAGACCGAAACCGCCTACGCCCTGGCCGACGCCTTGTACGGAGGTGAACGC
AACCTGATCAGCATCAACCTCTCGGAGTACCAGGAAGCCCACACCGTCAGCCAACTCAAAGGCGCCCCACCCGGCTACGT
CGGCTACGGCAGCGGCGGCGTGCTCACCGAAGCGGTGCGGCGCAAACCCTATTCGGTGGTGCTGCTGGATGAAATCGAAA
AGGCCCACCCGGATGTGCTCGAAGCCTTCTACAACGTCTTCGACAAAGGCCTGATGGAAGATGGCACCGGCCTGGTGGTG
GACTTCAAGAACACCGTGATGCTCGCCACCAGCAACGTCGGCGCCGAGCTATTGCTGGATACGCCCGCCGCGCAACTGGG
CAGCGACGCCTTCAACGAAGCCTTGCACAAGGTGCTGCTGCAAGCATTCCGCCCGGCGTTTTTGGCGCGCATGACCGTGG
TGGCATACCGGCCGCTGGATGAAGCGACACTGGAGGGGATCGTGCTGGCGAAGCTGGAAAAACTGCGCGGGCGCTACAAG
GCTGCGACGGGCAAGCAGTTCGAGTTCGATGCCGGAATCGTCAAGGCTGTGTTGGCCAAATGCAGCGCGGCGGGGGCACG
GGACGTCGAGAATGTGTTGATGACGCAAGTGACAGGGAAGTTGGCGGAGTGGGTGTTGGAATAG					