SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP004477
Component protein type	TssD
T6SS ID (Type)	T6SS00431 (Type i4a)
Strain	Pseudomonas brassicacearum subsp. brassicacearum NFM421
Replicon	chromosome
Sequence	Protein sequence (166 a.a.); Nucleotide sequence (498 bp)
Reference	[1] Ortet P, Barakat M, Lalaouna D, et al. Complete genome sequence of a beneficial plant root-associated bacterium, Pseudomonas brassicacearum. J Bacteriol. 2011 Jun;193(12):3146. doi: 10.1128/JB.00411-11. Epub 2011 Apr 22. PMID: 21515771 [2] Lalaouna D, Fochesato S, Sanchez L, et al. Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs. Appl Environ Microbiol. 2012 Mar;78(6):1658-65. doi: 10.1128/AEM.06769-11. Epub 2012 Jan 13. PMID: 22247157

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSEBR_RS17635
Coordinate (Strand)	4102906..4103403 (+)
NCBI ID	WP_003202803.1
RefSeq	NC_015379
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	4.5e-30	8..138

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 166 a.a.

						>T6CP004477 NC_015379:4102906-4103403 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssD]
MDAIILDLGGDIKGDSLLEGFTDKIEVMSYSHNVAMQVTNDVSNSERTSGRPHIGEFTLTKFVDSSTPTLNEYCCAGKPI
PQAVITVGRNAAEGSGKLMPFIIYTLTNVVLSNVSVSGGTGGKPVETLSLNFTKIKWELTAQKDDGTKEGTAGTTWDLAA
NKMTK*					

Nucleotide sequence: 498 bp

						>T6CP004477 NC_015379:4102906-4103403 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssD]
ATGGATGCAATCATTCTCGACCTCGGCGGCGACATCAAGGGCGACAGCCTGCTGGAGGGTTTCACGGACAAGATCGAGGT
CATGTCCTACAGCCACAACGTGGCGATGCAAGTCACCAACGACGTCAGCAACTCGGAGCGCACCTCCGGCCGACCCCACA
TCGGCGAGTTCACCCTGACCAAATTCGTCGACAGCTCCACCCCCACCCTCAATGAGTATTGCTGCGCCGGCAAGCCGATC
CCGCAAGCCGTCATCACCGTTGGCCGCAATGCCGCCGAGGGCAGCGGCAAACTCATGCCGTTCATCATCTACACCCTGAC
CAACGTCGTGCTGTCCAACGTCAGTGTCAGCGGCGGCACCGGCGGCAAACCGGTGGAAACCCTGTCGCTGAACTTCACCA
AGATCAAATGGGAGCTCACCGCGCAGAAAGACGACGGCACCAAGGAAGGCACGGCGGGCACGACCTGGGACCTGGCCGCC
AACAAAATGACCAAGTAG					