SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP004473
Component protein type	TssM
T6SS ID (Type)	T6SS00431 (Type i4a)
Strain	Pseudomonas brassicacearum subsp. brassicacearum NFM421
Replicon	chromosome
Sequence	Protein sequence (1275 a.a.); Nucleotide sequence (3825 bp)
Reference	[1] Ortet P, Barakat M, Lalaouna D, et al. Complete genome sequence of a beneficial plant root-associated bacterium, Pseudomonas brassicacearum. J Bacteriol. 2011 Jun;193(12):3146. doi: 10.1128/JB.00411-11. Epub 2011 Apr 22. PMID: 21515771 [2] Lalaouna D, Fochesato S, Sanchez L, et al. Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs. Appl Environ Microbiol. 2012 Mar;78(6):1658-65. doi: 10.1128/AEM.06769-11. Epub 2012 Jan 13. PMID: 22247157

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSEBR_RS17605
Coordinate (Strand)	4093970..4097794 (-)
NCBI ID	WP_003202790.1
RefSeq	NC_015379
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	3.1e-43	114..350

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 1275 a.a.

						>T6CP004473 NC_015379:c4097794-4093970 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssM]
MSTLSIVALVVLAIVVVLLIAALIWWLRTQGGAAIRSFYGAVRRMEQEQGTRDRYQMPWLMMLGNESDGAQLCAQWRLQP
TDKAAWFGRWFSDAEGSVLVVPQALFLPDEGMNRQRGNWWRLLGLMLRLRSKRPLDAVIWTVPFSTLDDIEHTTELSLKV
RRCFIDLLQRFGLSLPVYVVITGMEELPGVQELVSALPAEARESMLGWSSPYLPDAAWQSQWSDQALDQVNAALSQSIIE
IGALSGQLGSDLYGLPERFEGLRRALQILLEPVFQGNAQGEAPRCRGVYFTASQAPAVSGDGLTAADSAQRQTVFARQLW
SRRLVAERGLAQPVPRLLRLRQRGHRLVGAVALVVGLVWLGGMVWVWHDSVEEAEGLSRLILSTHKNHLVVDPDEPQLEP
TRHNVQNYWNMLEKAPRWRFVSVVFPTSWFSSVDTQLENGLRLTARHHWILPLRDLLNSDLEQLKSIRNTERRGNVENED
PAQWQSYVKAKALVDLAVRLEQHNQMFSQVVNDPKAPLDELVQLSNTALSLGLNTGTLSRPRFYNRVLFDAQNSDLKGLD
LNAARPVISDNFTGLMQRWLDHYFLADNFVRQAGYLKLHLQRLEAGGGNSLSELEDLMALIDDLQTLVSLTNSAWGRGKG
QDLVPGYSQMLDKVSHSTLLGPDIEQDLESQAAKLQQSFRDQWIVQTGSRDNLLVQQGSGLLVLQDHVTALDGAVQALFK
RDFVALAMQKDPSGNNPDSMGQGDGSDDFSVALNYFASYKSYANEEMPRIPPDYRDALMQAAEGAAARAMWLSLDESDEA
LPGMGFNVQTTQAVALQKAFMDVHRGDLAIRFQRLLNRRALAQLKSGLDEIDAQPLFSQRADVQRWDGSKNFGLQLYGAS
DPQDLKLNLAQQFGAMLQIAEQRMPALEWLIVQQDNLSALEHDQVARFMALNDELLKYKNQNPASSAAQLEQLVSRDFIA
MDTTSCAQILQTSSLPGGRGDLALRTVALQQGALQRCLYLQQSQAATAWNDVANYFNQYLAGRFPFSQDVRASDADPARV
QRLLELIDTRLPLAQAGLTLSQTPERLAAEDFLNRLKQAGTWLGPLFVRDKSGILGVEMDVRWRTDRDEERGADQVIAWS
LDAGNQQISYPGGGQQNLRWMVGQPVRLTLRWARNGYQRPANDPLQPNLVVRDLEAGWEYQGPWSLLRLMRSLVSVQRQP
NVDYTDFPLALQLPVTAQTQASTAQQTLMFMRLSLMSQGSKLPLSIPPLPTRAPRSPFMATASSPAIATREEGL*					

Nucleotide sequence: 3825 bp

						>T6CP004473 NC_015379:c4097794-4093970 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssM]
ATGAGCACCCTGAGTATCGTTGCCCTGGTGGTGCTCGCCATCGTCGTGGTGCTGTTGATCGCGGCCCTGATCTGGTGGCT
GCGGACCCAAGGCGGAGCGGCGATCCGCAGTTTCTATGGGGCGGTGCGCCGCATGGAACAGGAGCAGGGCACCCGGGACC
GCTATCAGATGCCTTGGCTGATGATGCTCGGTAACGAAAGCGACGGCGCCCAACTGTGCGCCCAGTGGCGCTTGCAACCG
ACCGACAAGGCCGCCTGGTTCGGGCGCTGGTTTTCCGATGCCGAGGGCTCGGTGCTGGTGGTGCCCCAGGCGTTGTTCCT
GCCCGATGAAGGCATGAACCGGCAACGGGGTAATTGGTGGCGCCTGTTGGGGCTGATGTTGCGCTTGCGCAGCAAGCGAC
CATTGGATGCGGTGATCTGGACGGTGCCGTTCAGCACCCTCGATGACATCGAACACACCACCGAACTGAGCCTCAAGGTC
CGGCGGTGCTTTATCGACCTGTTGCAACGGTTCGGCCTCAGCCTGCCGGTGTACGTGGTGATCACCGGGATGGAAGAGCT
GCCCGGGGTCCAGGAACTGGTCAGCGCGCTGCCCGCCGAGGCGCGTGAATCGATGCTGGGCTGGTCCTCGCCCTATTTGC
CGGATGCGGCCTGGCAGTCGCAGTGGAGTGACCAGGCCCTGGACCAGGTCAACGCGGCGCTGTCGCAATCGATCATCGAG
ATCGGTGCGCTGTCGGGGCAATTGGGCAGCGATCTGTATGGGTTGCCGGAGCGCTTCGAAGGCTTGCGACGGGCCTTGCA
GATCCTGTTGGAGCCGGTGTTCCAGGGCAATGCCCAAGGCGAAGCGCCGCGTTGTCGCGGGGTGTATTTCACTGCCAGCC
AGGCGCCGGCTGTCAGCGGTGATGGTTTGACCGCCGCCGACAGTGCCCAGCGGCAAACCGTGTTCGCCCGTCAATTGTGG
TCACGACGGCTGGTGGCCGAGCGTGGCCTGGCCCAGCCCGTGCCGCGGCTGTTGCGCCTGCGCCAGCGCGGGCATCGGCT
GGTCGGAGCGGTGGCGTTGGTGGTGGGCCTGGTCTGGTTGGGTGGCATGGTCTGGGTCTGGCACGATTCGGTGGAAGAAG
CCGAAGGGCTGTCGCGGTTGATCCTCAGCACCCATAAGAATCACCTCGTGGTCGATCCTGACGAACCGCAGCTGGAACCG
ACCCGGCACAACGTGCAGAACTATTGGAACATGTTGGAAAAGGCCCCGCGCTGGCGCTTTGTCTCGGTGGTCTTTCCCAC
TTCGTGGTTCTCTTCCGTGGACACGCAGCTGGAGAATGGGTTGCGCCTGACTGCTCGGCACCATTGGATATTGCCTCTGC
GGGACCTGCTCAATTCAGACCTGGAACAGCTCAAATCCATCCGCAACACCGAGCGGCGGGGCAATGTGGAAAACGAAGAT
CCGGCGCAATGGCAGAGCTACGTGAAGGCCAAGGCGCTGGTGGACCTGGCGGTACGCCTTGAACAGCACAACCAGATGTT
CAGCCAGGTGGTGAACGATCCCAAGGCACCGCTGGACGAACTGGTGCAACTGAGCAACACCGCCTTGTCCCTGGGGCTCA
ACACCGGCACCTTGAGCCGGCCCCGGTTCTATAACCGGGTGCTGTTCGATGCCCAGAACAGCGATTTGAAAGGGCTGGAC
CTGAACGCCGCGCGCCCGGTGATCTCCGATAACTTCACCGGCCTGATGCAGCGCTGGTTGGACCATTACTTCCTGGCGGA
CAACTTCGTGCGCCAGGCCGGCTACCTCAAACTGCACCTGCAACGGCTGGAGGCCGGCGGCGGCAACTCCTTGAGCGAAC
TCGAAGACCTGATGGCGCTGATCGACGATTTGCAGACCCTGGTCAGCCTGACCAACTCGGCCTGGGGGCGGGGCAAGGGC
CAGGACCTGGTCCCCGGCTACAGCCAGATGCTGGACAAGGTCAGCCACAGTACTTTGCTGGGGCCGGATATCGAGCAGGA
CCTGGAGAGCCAGGCGGCGAAGTTGCAGCAGAGCTTTCGTGACCAGTGGATCGTCCAGACCGGCTCCCGGGACAATCTGC
TGGTGCAACAGGGCAGTGGCCTTCTGGTCTTGCAAGACCATGTCACGGCGCTGGACGGTGCAGTGCAGGCTTTGTTCAAG
CGTGATTTCGTGGCGCTGGCCATGCAGAAGGATCCGTCGGGCAACAACCCCGATTCGATGGGCCAGGGCGATGGCAGCGA
TGATTTCAGCGTCGCGCTGAACTACTTCGCCAGCTACAAGAGCTATGCCAATGAAGAAATGCCGCGCATTCCGCCGGATT
ACCGTGACGCGCTGATGCAGGCCGCTGAAGGTGCCGCGGCCCGGGCCATGTGGTTGAGCCTGGATGAAAGCGACGAGGCG
TTGCCCGGCATGGGCTTCAATGTGCAAACGACCCAGGCCGTTGCGCTGCAAAAGGCCTTTATGGACGTGCACCGTGGCGA
CCTGGCGATCCGTTTCCAACGTCTGCTCAATCGCCGGGCATTGGCGCAGCTCAAGAGTGGCCTGGACGAAATCGATGCCC
AGCCGCTGTTCAGCCAGCGGGCCGATGTCCAGCGTTGGGATGGCTCGAAAAACTTCGGCTTGCAACTCTACGGTGCCAGC
GATCCACAAGACCTGAAGCTGAACCTGGCCCAGCAATTCGGCGCGATGCTGCAGATCGCCGAGCAGCGAATGCCCGCGCT
GGAATGGTTGATTGTCCAGCAGGACAACCTGTCGGCCCTGGAGCACGACCAGGTGGCACGCTTCATGGCCCTCAACGATG
AATTGCTCAAGTACAAGAACCAGAACCCGGCCAGCTCGGCGGCTCAGCTGGAACAATTGGTGAGTCGGGACTTTATCGCC
ATGGACACCACCTCCTGCGCGCAGATCTTGCAGACCTCCAGCCTGCCCGGTGGTCGCGGTGACCTGGCCCTGCGCACGGT
CGCGTTGCAGCAGGGCGCCCTGCAACGGTGCCTGTACCTGCAACAGAGCCAGGCGGCGACGGCCTGGAATGACGTGGCCA
ACTATTTCAATCAATACCTGGCCGGGCGCTTTCCGTTTTCTCAGGATGTGCGGGCCAGCGATGCCGACCCGGCGCGGGTC
CAGCGTTTGCTGGAACTGATTGACACACGCCTGCCCCTGGCCCAGGCCGGACTGACGCTGAGCCAGACGCCGGAGCGGCT
GGCGGCCGAGGATTTCCTCAACCGTTTGAAACAGGCCGGTACTTGGCTCGGGCCGCTGTTCGTGCGGGACAAGAGTGGCA
TTCTTGGGGTGGAAATGGACGTGCGCTGGCGTACCGATCGCGATGAGGAGCGCGGCGCCGACCAGGTGATCGCCTGGAGC
CTGGACGCCGGCAACCAACAGATCAGCTACCCCGGCGGCGGCCAGCAGAACCTGCGCTGGATGGTCGGCCAGCCCGTACG
CCTGACCTTGCGCTGGGCCCGTAACGGTTATCAGCGTCCGGCCAACGACCCGCTGCAACCGAACCTGGTGGTGCGCGACC
TGGAGGCCGGTTGGGAGTACCAGGGGCCCTGGTCGTTGCTGCGCCTGATGCGCTCGCTGGTGTCGGTCCAGCGCCAGCCA
AATGTCGACTACACCGATTTTCCCCTGGCCCTGCAACTGCCGGTGACCGCCCAGACCCAGGCCAGCACGGCGCAGCAAAC
GTTGATGTTCATGCGCCTGTCGTTGATGAGCCAGGGCTCCAAGCTGCCTTTGTCGATCCCACCGCTGCCGACGCGGGCGC
CCCGTTCACCTTTCATGGCGACCGCATCGAGCCCCGCCATCGCCACCCGTGAGGAGGGCCTGTGA					