Detailed information of component protein
Summary
| Component ID | T6CP004076 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00396  (Type i2) |
| Strain | Enterobacter cloacae subsp. cloacae ATCC 13047 |
| Replicon | chromosome |
| Sequence | Protein sequence (182 a.a.); Nucleotide sequence (546 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ECL_RS08965 |
| Coordinate (Strand) | 1843686..1844231 (+) |
| NCBI ID | WP_013096443.1 |
| RefSeq | NC_014121 |
| Uniprot ID | A0A0H3CI96_ENTCC |
| KEGG ID | enc:ECL_01819 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.7e-29 | 48..170 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-181 | VLRTSLIKPACWLPALLAFSLTGCGITQGITDGTKSAFNAVFYKKIKVLHLDFTAREALNTDSRESNSLSEPVVVRVYQLKDRKIFDKTVYQQLLKDGETILKADLLTSRDVVVKPGGDVSLDMPMEEEAHFVAVVGLFRHPDMVNNTWKQVLRREDLDPDKPRILEAGNNHLTLQPRKDD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9982 | pos: 23-24. LTG-CG. | VLRTSLIKPACWLPALLAFSLTG |
Protein sequence: 182 a.a.
>T6CP004076 NC_014121:1843686-1844231 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssJ]
VLRTSLIKPACWLPALLAFSLTGCGITQGITDGTKSAFNAVFYKKIKVLHLDFTAREALNTDSRESNSLSEPVVVRVYQL
KDRKIFDKTVYQQLLKDGETILKADLLTSRDVVVKPGGDVSLDMPMEEEAHFVAVVGLFRHPDMVNNTWKQVLRREDLDP
DKPRILEAGNNHLTLQPRKDD*
VLRTSLIKPACWLPALLAFSLTGCGITQGITDGTKSAFNAVFYKKIKVLHLDFTAREALNTDSRESNSLSEPVVVRVYQL
KDRKIFDKTVYQQLLKDGETILKADLLTSRDVVVKPGGDVSLDMPMEEEAHFVAVVGLFRHPDMVNNTWKQVLRREDLDP
DKPRILEAGNNHLTLQPRKDD*
Nucleotide sequence: 546 bp
>T6CP004076 NC_014121:1843686-1844231 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssJ]
GTGTTACGTACAAGTTTGATTAAACCCGCGTGCTGGCTGCCTGCACTGCTGGCGTTCAGCCTCACGGGCTGTGGGATTAC
GCAGGGTATCACTGATGGCACTAAATCGGCATTTAACGCTGTGTTTTATAAAAAAATTAAGGTACTGCATCTGGATTTCA
CCGCCCGTGAAGCGTTGAACACCGATTCCCGCGAAAGTAACTCGCTGTCTGAGCCGGTGGTAGTCCGCGTATATCAACTG
AAAGACCGCAAGATCTTCGACAAAACGGTCTATCAGCAGCTGCTGAAGGACGGGGAGACCATTCTTAAAGCGGACCTTCT
GACCAGCCGTGACGTGGTGGTGAAGCCGGGCGGAGATGTGAGCCTTGATATGCCGATGGAAGAAGAAGCGCATTTTGTCG
CGGTGGTCGGGCTGTTCCGCCATCCGGATATGGTTAACAACACCTGGAAACAGGTGCTCAGACGTGAAGATCTCGATCCG
GATAAACCGCGCATTCTTGAAGCCGGGAATAACCATCTGACATTACAACCGCGAAAGGATGACTGA
GTGTTACGTACAAGTTTGATTAAACCCGCGTGCTGGCTGCCTGCACTGCTGGCGTTCAGCCTCACGGGCTGTGGGATTAC
GCAGGGTATCACTGATGGCACTAAATCGGCATTTAACGCTGTGTTTTATAAAAAAATTAAGGTACTGCATCTGGATTTCA
CCGCCCGTGAAGCGTTGAACACCGATTCCCGCGAAAGTAACTCGCTGTCTGAGCCGGTGGTAGTCCGCGTATATCAACTG
AAAGACCGCAAGATCTTCGACAAAACGGTCTATCAGCAGCTGCTGAAGGACGGGGAGACCATTCTTAAAGCGGACCTTCT
GACCAGCCGTGACGTGGTGGTGAAGCCGGGCGGAGATGTGAGCCTTGATATGCCGATGGAAGAAGAAGCGCATTTTGTCG
CGGTGGTCGGGCTGTTCCGCCATCCGGATATGGTTAACAACACCTGGAAACAGGTGCTCAGACGTGAAGATCTCGATCCG
GATAAACCGCGCATTCTTGAAGCCGGGAATAACCATCTGACATTACAACCGCGAAAGGATGACTGA