Detailed information of component protein
Summary
| Component ID | T6CP004069 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS00396  (Type i2) |
| Strain | Enterobacter cloacae subsp. cloacae ATCC 13047 |
| Replicon | chromosome |
| Sequence | Protein sequence (164 a.a.); Nucleotide sequence (492 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ECL_RS08875 |
| Coordinate (Strand) | 1819466..1819957 (+) |
| NCBI ID | WP_013096428.1 |
| RefSeq | NC_014121 |
| Uniprot ID | A0A0M7BUW3_ENTCL, A0A0H3CHI4_ENTCC |
| KEGG ID | enc:ECL_01801 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 1.5e-52 | 7..157 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-163 | MADTFQNEVPKARINLKLALHTGGAQKKVELPLKLLTVGDFSNGKESRPLSERKKINVNKNNLNSVLSEFNPEVNMTVPNTLAGERTEENVTLRFSDMKDFEPEQVARQIPQLRAMLAMRNLLRDLKSNLLDNATFRKELEKILKDPALSQELRNEMSALAPK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9929 | - | - |
Protein sequence: 164 a.a.
>T6CP004069 NC_014121:1819466-1819957 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssB]
MADTFQNEVPKARINLKLALHTGGAQKKVELPLKLLTVGDFSNGKESRPLSERKKINVNKNNLNSVLSEFNPEVNMTVPN
TLAGERTEENVTLRFSDMKDFEPEQVARQIPQLRAMLAMRNLLRDLKSNLLDNATFRKELEKILKDPALSQELRNEMSAL
APK*
MADTFQNEVPKARINLKLALHTGGAQKKVELPLKLLTVGDFSNGKESRPLSERKKINVNKNNLNSVLSEFNPEVNMTVPN
TLAGERTEENVTLRFSDMKDFEPEQVARQIPQLRAMLAMRNLLRDLKSNLLDNATFRKELEKILKDPALSQELRNEMSAL
APK*
Nucleotide sequence: 492 bp
>T6CP004069 NC_014121:1819466-1819957 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssB]
ATGGCTGATACGTTCCAGAACGAAGTGCCTAAGGCACGCATTAATTTAAAACTTGCATTGCATACCGGAGGGGCGCAGAA
GAAAGTTGAGTTACCCCTTAAATTACTCACTGTCGGTGATTTCAGTAACGGCAAAGAGAGCCGGCCATTATCTGAAAGAA
AGAAAATTAACGTCAACAAAAATAACCTCAATAGTGTACTTTCGGAATTTAACCCGGAGGTCAACATGACGGTTCCGAAT
ACGCTGGCGGGTGAGCGTACGGAAGAGAATGTGACGCTGCGCTTTTCAGATATGAAAGATTTTGAACCCGAACAGGTGGC
CCGACAAATTCCCCAACTTCGTGCCATGCTGGCGATGCGTAATTTATTACGTGACCTCAAGTCCAATCTGCTGGATAACG
CCACTTTCAGAAAAGAGCTCGAGAAAATTCTTAAGGATCCGGCGCTGTCTCAGGAATTACGTAACGAAATGAGTGCCCTG
GCCCCGAAATAA
ATGGCTGATACGTTCCAGAACGAAGTGCCTAAGGCACGCATTAATTTAAAACTTGCATTGCATACCGGAGGGGCGCAGAA
GAAAGTTGAGTTACCCCTTAAATTACTCACTGTCGGTGATTTCAGTAACGGCAAAGAGAGCCGGCCATTATCTGAAAGAA
AGAAAATTAACGTCAACAAAAATAACCTCAATAGTGTACTTTCGGAATTTAACCCGGAGGTCAACATGACGGTTCCGAAT
ACGCTGGCGGGTGAGCGTACGGAAGAGAATGTGACGCTGCGCTTTTCAGATATGAAAGATTTTGAACCCGAACAGGTGGC
CCGACAAATTCCCCAACTTCGTGCCATGCTGGCGATGCGTAATTTATTACGTGACCTCAAGTCCAATCTGCTGGATAACG
CCACTTTCAGAAAAGAGCTCGAGAAAATTCTTAAGGATCCGGCGCTGTCTCAGGAATTACGTAACGAAATGAGTGCCCTG
GCCCCGAAATAA