Detailed information of component protein


Summary

Component ID T6CP004067
Component protein type TssI
T6SS ID (Type) T6SS00395 experimental (Type i3)
Strain Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon chromosome
Sequence Protein sequence (643 a.a.); Nucleotide sequence (1929 bp)
Reference
[1] Whitney JC, Beck CM, Goo YA, et al. Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 2014 May;92(3):529-42. doi: 10.1111/mmi.12571. Epub 2014 Mar 28. PMID: 24589350
[2] Zhang H, Zhang H, Gao ZQ, et al. Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J Biol Chem. 2013 Feb 22;288(8):5928-39. doi: 10.1074/jbc.M112.434357. Epub 2013 Jan 3. PMID: 23288853
[3] Zhang H, Gao ZQ, Wei Y, et al. Insights into the cross-immunity mechanism within effector families of bacteria type VI secretion system from the structure of StTae4-EcTai4 complex. PLoS One. 2013 Sep 2;8(9):e73782. doi: 10.1371/journal.pone.0073782. eCollection 2013. PMID: 24023903
[4] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[5] Soria-Bustos J, Ares MA, Gómez-Aldapa CA, et al. Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization. Front Microbiol. 2020 Sep 24;11:560488. doi: 10.3389/fmicb.2020.560488. eCollection 2020. PMID: 33072020
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ECL_RS07645
Coordinate (Strand) 1582007..1583935 (+)
NCBI ID WP_013096197.1
RefSeq NC_014121
Uniprot ID A0A0H3CIZ3_ENTCC
KEGG ID enc:ECL_01558
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 2.7e-87 22..327
Phage_base_V PF04717.14 2.6e-15 381..448
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-642 MLNRITVQLPVEGLLFWKLSGREAMSESFALTLTLLGTDARIDRSKLLGQPVTVTIPTQNLLTPRYINGKVTRVAVSAVELTGTRYAVYQLTVEPDLWPMKRDRNLRIFQGQTVPQIVKTLLGEHQVNVEDKLTGSYRVWDYCVQYQESSLDFISRLMELEGIAYYFSHEADKHTLVLTDAATQHQPFSGYEVIPYHQTPSGGSTDEEGISQWALEDSVTPGIYSLDDYDFRKPNAWLFQAQQNPASPKPGSIDVYDWPGRFVETGHAEFYARIRQERWQVEHQQIQATATAAGIAPGHTFTLTNAPFFSDNGDYLVTAAGYHLEENRYASGEGETIHRIDFTVIPASVSFRPAQSTAWPRTYGPQTAKVVGPQGESIWTDKYGRVKVKFHWDRLAKGDDTSSCWVRVSSAWAGQGYGGVQIPRVGDEVVVDFINGDPDRPIITGRVYNDASMPPWALPAAATQMGFMSRTKDGSVDNANALRFEDKAGAEQVWLQAERNLDINVKNDESHATEKNRTQYVGEDETLRVAKNQKAGVKGDVVCLTGNSRSDKVVNDFIISAGNTLRLECGESAIELSKDGSVNIIGNNFNFTAKQSAQINTLSGELHLNPADGSNAIDAPGASHQTAIQQEVDGFFVFNANK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9454 - -


  Protein sequence: 643 a.a.    

>T6CP004067 NC_014121:1582007-1583935 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssI]
MLNRITVQLPVEGLLFWKLSGREAMSESFALTLTLLGTDARIDRSKLLGQPVTVTIPTQNLLTPRYINGKVTRVAVSAVE
LTGTRYAVYQLTVEPDLWPMKRDRNLRIFQGQTVPQIVKTLLGEHQVNVEDKLTGSYRVWDYCVQYQESSLDFISRLMEL
EGIAYYFSHEADKHTLVLTDAATQHQPFSGYEVIPYHQTPSGGSTDEEGISQWALEDSVTPGIYSLDDYDFRKPNAWLFQ
AQQNPASPKPGSIDVYDWPGRFVETGHAEFYARIRQERWQVEHQQIQATATAAGIAPGHTFTLTNAPFFSDNGDYLVTAA
GYHLEENRYASGEGETIHRIDFTVIPASVSFRPAQSTAWPRTYGPQTAKVVGPQGESIWTDKYGRVKVKFHWDRLAKGDD
TSSCWVRVSSAWAGQGYGGVQIPRVGDEVVVDFINGDPDRPIITGRVYNDASMPPWALPAAATQMGFMSRTKDGSVDNAN
ALRFEDKAGAEQVWLQAERNLDINVKNDESHATEKNRTQYVGEDETLRVAKNQKAGVKGDVVCLTGNSRSDKVVNDFIIS
AGNTLRLECGESAIELSKDGSVNIIGNNFNFTAKQSAQINTLSGELHLNPADGSNAIDAPGASHQTAIQQEVDGFFVFNA
NK*

  Nucleotide sequence: 1929 bp    

>T6CP004067 NC_014121:1582007-1583935 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssI]
ATGCTCAACCGAATTACCGTTCAGCTCCCCGTCGAGGGGCTGCTTTTCTGGAAACTCTCCGGCCGCGAGGCGATGTCCGA
GTCGTTCGCCCTGACGCTGACCCTGCTCGGCACGGATGCGCGTATCGACCGCAGCAAACTGCTGGGCCAGCCGGTCACGG
TGACCATCCCCACCCAGAACCTGCTTACCCCGCGCTACATCAACGGTAAAGTCACGCGCGTGGCGGTGAGCGCCGTTGAG
CTGACGGGCACACGCTATGCGGTGTATCAGCTGACGGTGGAGCCGGACCTGTGGCCGATGAAGCGCGACCGTAACCTGCG
TATCTTCCAGGGGCAGACGGTGCCGCAGATTGTGAAAACCCTGCTGGGCGAGCATCAGGTCAACGTGGAAGATAAGCTCA
CCGGCAGCTACCGGGTGTGGGACTACTGTGTCCAGTATCAGGAGTCGAGCCTGGACTTCATCAGCCGCCTGATGGAGCTG
GAGGGGATAGCGTATTACTTCAGCCACGAGGCCGACAAACACACCCTGGTGCTCACCGATGCCGCCACGCAGCATCAGCC
GTTCAGCGGCTACGAGGTCATCCCCTATCACCAGACGCCGTCCGGCGGCAGCACGGACGAAGAGGGTATCAGCCAGTGGG
CGCTGGAGGACAGCGTGACGCCGGGCATTTACAGCCTCGACGACTACGACTTCCGCAAGCCGAACGCGTGGCTGTTCCAG
GCGCAGCAGAACCCGGCCTCGCCGAAGCCAGGCAGCATTGACGTGTACGACTGGCCGGGGCGCTTTGTGGAGACCGGGCA
TGCGGAGTTCTACGCCCGCATTCGTCAGGAGCGCTGGCAGGTGGAGCATCAGCAGATTCAGGCCACGGCCACGGCGGCGG
GTATTGCGCCGGGCCACACCTTCACCCTGACCAACGCACCGTTCTTCAGCGATAACGGTGACTATCTGGTGACGGCGGCC
GGGTATCATCTGGAAGAGAACCGCTATGCCAGCGGCGAAGGGGAGACCATCCACCGCATCGATTTCACGGTTATCCCGGC
GTCGGTGTCGTTCCGTCCGGCGCAGTCCACGGCGTGGCCGCGTACCTACGGCCCGCAGACGGCGAAGGTTGTCGGGCCCC
AGGGAGAGAGCATCTGGACGGACAAATATGGCCGCGTGAAGGTGAAATTCCACTGGGACCGTCTGGCAAAAGGGGACGAC
ACCAGCTCCTGCTGGGTGCGCGTGTCGAGCGCCTGGGCGGGCCAGGGCTACGGTGGGGTGCAGATCCCGCGCGTGGGCGA
TGAGGTGGTGGTGGACTTTATCAACGGTGACCCGGACCGTCCTATTATCACCGGGCGCGTGTACAACGATGCCAGCATGC
CACCGTGGGCGCTGCCGGCGGCGGCGACGCAGATGGGCTTTATGAGCCGCACGAAAGATGGCTCCGTGGATAACGCCAAC
GCCCTGCGGTTTGAGGACAAGGCGGGTGCGGAACAGGTGTGGCTCCAGGCCGAGCGCAATCTCGATATCAACGTCAAAAA
TGATGAATCGCATGCCACGGAAAAAAATCGAACCCAATATGTGGGAGAGGATGAAACCTTACGGGTGGCAAAAAATCAGA
AGGCAGGGGTTAAAGGGGATGTGGTCTGCCTGACGGGCAACAGCCGCAGCGATAAAGTTGTTAATGACTTTATTATTTCA
GCGGGCAATACGTTGAGGCTGGAGTGTGGTGAAAGCGCTATCGAATTATCAAAAGACGGGAGCGTGAATATCATTGGCAA
TAACTTTAACTTTACCGCAAAACAGAGTGCGCAAATAAACACGCTTTCCGGGGAATTGCATCTTAACCCTGCTGATGGCA
GCAATGCCATCGATGCGCCAGGGGCATCTCACCAGACAGCAATCCAGCAGGAAGTGGATGGTTTTTTTGTTTTCAATGCT
AATAAGTAA