Detailed information of component protein


Summary

Component ID T6CP004066
Component protein type TssH
T6SS ID (Type) T6SS00395 experimental (Type i3)
Strain Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon chromosome
Sequence Protein sequence (872 a.a.); Nucleotide sequence (2616 bp)
Reference
[1] Whitney JC, Beck CM, Goo YA, et al. Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 2014 May;92(3):529-42. doi: 10.1111/mmi.12571. Epub 2014 Mar 28. PMID: 24589350
[2] Zhang H, Zhang H, Gao ZQ, et al. Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J Biol Chem. 2013 Feb 22;288(8):5928-39. doi: 10.1074/jbc.M112.434357. Epub 2013 Jan 3. PMID: 23288853
[3] Zhang H, Gao ZQ, Wei Y, et al. Insights into the cross-immunity mechanism within effector families of bacteria type VI secretion system from the structure of StTae4-EcTai4 complex. PLoS One. 2013 Sep 2;8(9):e73782. doi: 10.1371/journal.pone.0073782. eCollection 2013. PMID: 24023903
[4] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[5] Soria-Bustos J, Ares MA, Gómez-Aldapa CA, et al. Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization. Front Microbiol. 2020 Sep 24;11:560488. doi: 10.3389/fmicb.2020.560488. eCollection 2020. PMID: 33072020
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ECL_RS07605
Coordinate (Strand) 1572298..1574913 (+)
NCBI ID WP_013096191.1
RefSeq NC_014121
Uniprot ID A0A0H3CHI7_ENTCC
KEGG ID enc:ECL_01551
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 4.5e-40 602..767
AAA_lid_9 PF17871.3 1.1e-25 363..454
ClpB_D2-small PF10431.11 1.1e-13 775..847
AAA PF00004.31 3.2e-10 223..355
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-871 MSEISRAVLFGKLDTLLFTSLESATAFCKLRGNPYVELVHWLHQLMQQQDGDLQQVIRHFALDEQQLTRDIVAALDALPRGASSVSDLSEHIDSAVERAWVYGSLKFGVSRIRGGHLLIGILKTWNLANVLKSISAQFTRLNVEVLIEQFDTICANSKETQQAAAAVDAPAGSVPAAQGTLAQYGQDLTARAREGKIDPVVGRDEEIRQMIDILMRRRQNNPLLTGEAGVGKTAVVEGLALRIADGDVPEPLQNIQLWLLDIGMLQAGAGMKGEFEARLQALINEVQSSATPVILFIDEIHTLIGAGGQQGTGDAANLLKPALARGQLRTIGATTWAEYKKYIEKDPALTRRFQTVQVHEPDEAKAVLMLRSTVSPLETHHQVLLLDEAVSAAVKLSHRYIPARQLPDKAVALLDTACARVAVSQSAPPAQLEDCLRHLAALDVEIEIAEREARVGAGESERVTALTAERDAYEAKREALARRWEDERSLVQEIIRLRAALFAAGDEDTAELRSQLAEQQQALNALQGDEPLLFAAVDENVVAAVVSDWTGIPLGRMVKNEIDAVLNLADTLNQRVIGQRHGLDLIARRVKTSRAKLDDPNKPVGVFMLCGPSGVGKTETALALAESLYGGEQNVITINMSEFQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEIEKAHPDVHEIFFQVFDKGWMEDGEGRHIDFRNTIIILTSNVGTELISAMCADPELMPEPEALSGALRQPLLEVFPPALLGRLLVVPYYPLSDAMLGQIVRLQLKRIQRRLEENHNIISEFDDSVVEQIVQRCTEVESGGRMVDAILTNTLLPQMSQILLTASRSDEQYRRLHVTCEQGEFHCQFAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9627 - -


  Protein sequence: 872 a.a.    

>T6CP004066 NC_014121:1572298-1574913 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssH]
MSEISRAVLFGKLDTLLFTSLESATAFCKLRGNPYVELVHWLHQLMQQQDGDLQQVIRHFALDEQQLTRDIVAALDALPR
GASSVSDLSEHIDSAVERAWVYGSLKFGVSRIRGGHLLIGILKTWNLANVLKSISAQFTRLNVEVLIEQFDTICANSKET
QQAAAAVDAPAGSVPAAQGTLAQYGQDLTARAREGKIDPVVGRDEEIRQMIDILMRRRQNNPLLTGEAGVGKTAVVEGLA
LRIADGDVPEPLQNIQLWLLDIGMLQAGAGMKGEFEARLQALINEVQSSATPVILFIDEIHTLIGAGGQQGTGDAANLLK
PALARGQLRTIGATTWAEYKKYIEKDPALTRRFQTVQVHEPDEAKAVLMLRSTVSPLETHHQVLLLDEAVSAAVKLSHRY
IPARQLPDKAVALLDTACARVAVSQSAPPAQLEDCLRHLAALDVEIEIAEREARVGAGESERVTALTAERDAYEAKREAL
ARRWEDERSLVQEIIRLRAALFAAGDEDTAELRSQLAEQQQALNALQGDEPLLFAAVDENVVAAVVSDWTGIPLGRMVKN
EIDAVLNLADTLNQRVIGQRHGLDLIARRVKTSRAKLDDPNKPVGVFMLCGPSGVGKTETALALAESLYGGEQNVITINM
SEFQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEIEKAHPDVHEIFFQVFDKGWMEDGEGRHIDFRNTII
ILTSNVGTELISAMCADPELMPEPEALSGALRQPLLEVFPPALLGRLLVVPYYPLSDAMLGQIVRLQLKRIQRRLEENHN
IISEFDDSVVEQIVQRCTEVESGGRMVDAILTNTLLPQMSQILLTASRSDEQYRRLHVTCEQGEFHCQFAA*

  Nucleotide sequence: 2616 bp    

>T6CP004066 NC_014121:1572298-1574913 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssH]
ATGTCAGAAATTAGCCGTGCCGTGCTTTTCGGCAAACTGGATACGCTGTTATTTACCTCTCTGGAAAGCGCCACCGCGTT
CTGCAAGCTGCGCGGCAACCCCTACGTCGAGCTGGTGCACTGGCTGCATCAGTTGATGCAGCAGCAGGATGGCGATCTGC
AACAGGTGATCCGTCATTTTGCCCTCGACGAACAGCAGCTGACGCGCGATATCGTGGCGGCGCTCGATGCCCTGCCGCGC
GGAGCCAGTTCCGTTTCCGATCTCTCCGAACATATCGACAGCGCCGTCGAGCGCGCCTGGGTCTATGGATCGCTGAAGTT
TGGCGTCAGCCGTATCCGCGGCGGCCATCTGCTGATCGGCATTCTGAAAACCTGGAATCTGGCGAACGTGCTGAAGAGCA
TCTCGGCGCAGTTTACCCGTCTTAACGTCGAGGTGCTGATTGAGCAGTTCGATACTATCTGTGCTAACAGCAAAGAGACG
CAGCAGGCCGCTGCCGCAGTGGATGCGCCAGCCGGTTCGGTGCCTGCTGCCCAGGGGACGCTGGCCCAGTATGGTCAGGA
CCTGACCGCCCGTGCCCGTGAAGGCAAAATTGACCCGGTGGTTGGGCGCGATGAAGAGATCCGCCAGATGATCGATATCC
TGATGCGTCGCCGCCAGAACAACCCGCTGCTGACCGGGGAAGCCGGCGTCGGAAAAACGGCGGTGGTGGAAGGGCTGGCG
CTGCGCATCGCCGACGGCGATGTGCCGGAGCCGCTGCAAAACATTCAGCTGTGGCTGCTGGATATCGGTATGCTGCAGGC
CGGTGCCGGGATGAAAGGCGAGTTCGAAGCCCGTCTGCAGGCATTAATCAATGAAGTGCAGTCCAGCGCCACGCCAGTCA
TTCTGTTTATCGATGAAATCCACACCCTGATCGGTGCGGGCGGTCAGCAGGGCACCGGTGATGCCGCCAACCTGCTGAAG
CCAGCGCTGGCGCGTGGACAACTGCGTACCATTGGCGCCACTACCTGGGCGGAATACAAAAAATACATCGAGAAAGATCC
GGCACTGACCCGTCGCTTCCAGACGGTGCAGGTACATGAACCCGATGAGGCCAAAGCGGTTCTGATGCTGCGCAGCACCG
TGTCGCCGCTGGAGACCCACCACCAGGTCTTACTGCTCGACGAAGCGGTTAGCGCAGCCGTGAAGCTTTCGCATCGCTAC
ATTCCGGCCCGTCAGTTGCCGGATAAAGCCGTTGCGCTGCTGGATACGGCCTGCGCCCGCGTGGCGGTGAGCCAGAGTGC
ACCTCCGGCGCAGCTGGAAGATTGCCTGCGCCATCTTGCGGCGCTGGACGTAGAAATTGAGATAGCGGAGCGCGAAGCGC
GCGTCGGTGCAGGGGAATCTGAGCGCGTCACGGCATTAACCGCAGAGCGTGACGCGTATGAGGCCAAACGTGAGGCCCTG
GCCCGGCGCTGGGAAGACGAGCGTAGCCTGGTGCAGGAGATCATTCGCCTGCGTGCCGCACTGTTTGCGGCGGGTGATGA
GGATACCGCTGAGCTGCGCAGCCAACTGGCAGAGCAGCAGCAGGCGCTGAACGCCTTACAGGGTGATGAACCGTTGCTGT
TTGCCGCTGTGGATGAAAACGTGGTGGCGGCCGTAGTCTCTGACTGGACCGGCATCCCGCTGGGCCGGATGGTGAAAAAC
GAGATTGATGCGGTACTGAACCTCGCCGACACGCTGAACCAGCGGGTGATTGGCCAGCGTCACGGTCTGGATCTGATTGC
CCGCCGGGTGAAAACCTCCCGGGCAAAACTCGATGATCCGAACAAGCCGGTTGGCGTGTTTATGCTCTGTGGCCCGTCCG
GCGTGGGGAAAACCGAAACCGCGCTGGCGCTGGCGGAGTCGCTGTACGGTGGTGAGCAGAACGTCATTACCATCAATATG
AGTGAATTCCAGGAAGCGCACACCGTTTCCACCTTAAAAGGTGCACCCCCGGGATACGTCGGGTATGGTGAAGGCGGGGT
GTTAACCGAGGCCGTCCGTCGCCGTCCTTACAGCGTGGTGCTGCTGGACGAAATCGAAAAAGCGCACCCGGACGTACATG
AGATCTTCTTCCAGGTCTTCGACAAAGGCTGGATGGAGGATGGCGAAGGTCGTCACATTGATTTCCGTAATACGATTATT
ATTCTGACGTCTAACGTCGGGACCGAGCTTATCAGCGCCATGTGTGCCGATCCGGAACTGATGCCGGAGCCGGAGGCGTT
AAGCGGGGCGCTGCGCCAGCCGCTGCTGGAGGTGTTCCCACCGGCGCTGCTGGGCCGTCTGCTGGTGGTGCCGTACTATC
CGCTGAGCGACGCGATGCTGGGGCAGATTGTTCGCCTGCAGCTCAAACGCATTCAGCGTCGGCTGGAAGAGAATCACAAT
ATTATTTCCGAGTTTGATGACAGCGTTGTAGAACAGATTGTTCAGCGCTGTACCGAGGTGGAATCAGGTGGACGTATGGT
GGATGCGATCCTGACCAACACCTTGCTGCCTCAGATGAGTCAGATATTACTTACCGCCAGCCGCAGCGACGAGCAGTACC
GACGTCTGCATGTCACCTGCGAGCAGGGCGAGTTTCACTGTCAGTTTGCCGCGTAA