SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003921
Component protein type	TssJ
T6SS ID (Type)	T6SS00382 (Type i3)
Strain	Xenorhabdus bovienii SS-2004
Replicon	chromosome
Sequence	Protein sequence (171 a.a.); Nucleotide sequence (513 bp)
Reference	[1] Kochanowsky RM, Bradshaw C, Forlastro I, et al. Xenorhabdus bovienii strain jolietti uses a type 6 secretion system to kill closely related Xenorhabdus strains. FEMS Microbiol Ecol. 2020 Aug 1;96(8). pii: fiaa073. doi: 10.1093/femsec/fiaa073. PMID: 32558899

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	XBJ1_RS08970
Coordinate (Strand)	2071856..2072368 (-)
NCBI ID	WP_143827727.1
RefSeq	NC_013892
Uniprot ID	UPI00117D83C2
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.1e-20	36..135

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-3	MMV
Transmembrane helix	4-26	ILFLHVLVFNLIIFNIFVLTGCS
Inside	27-170	SSEQKKSPPYQLIFRATNNVNDSAPLKIHVILLKSTSEFMSADFFSLQGHAQAVLGNKLINSEQLFLLPFQHKYFLLEKNIPEANSIGIFAEYKQLDGKKWRIALPVPIPEPPAFYKFWTSPPDTLDICIQVTANGLNFIDKCN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9825	pos: 24-25. LTG-CS.	MMVILFLHVLVFNLIIFNIFVLTG

Protein sequence: 171 a.a.

						>T6CP003921 NC_013892:c2072368-2071856 [Xenorhabdus bovienii SS-2004] [TssJ]
MMVILFLHVLVFNLIIFNIFVLTGCSSSEQKKSPPYQLIFRATNNVNDSAPLKIHVILLKSTSEFMSADFFSLQGHAQAV
LGNKLINSEQLFLLPFQHKYFLLEKNIPEANSIGIFAEYKQLDGKKWRIALPVPIPEPPAFYKFWTSPPDTLDICIQVTA
NGLNFIDKCN*					

Nucleotide sequence: 513 bp

						>T6CP003921 NC_013892:c2072368-2071856 [Xenorhabdus bovienii SS-2004] [TssJ]
ATGATGGTAATACTGTTTCTTCACGTACTCGTATTTAATTTAATAATATTTAACATCTTCGTATTAACAGGGTGTTCATC
ATCCGAGCAGAAAAAATCGCCCCCTTATCAATTGATTTTTCGTGCCACAAATAATGTGAATGACTCAGCTCCGCTGAAAA
TACACGTTATTTTGTTGAAATCGACTTCAGAGTTTATGTCTGCTGATTTTTTTTCACTGCAAGGTCATGCTCAGGCTGTA
CTGGGTAATAAATTGATTAATTCTGAGCAACTTTTTTTACTGCCTTTTCAGCACAAATATTTCTTGTTGGAAAAAAACAT
ACCAGAAGCCAATTCTATCGGCATTTTTGCTGAATATAAGCAATTGGATGGGAAAAAATGGCGTATCGCATTACCGGTTC
CCATTCCTGAGCCTCCCGCTTTCTATAAATTTTGGACATCACCGCCAGACACATTAGATATATGCATTCAAGTCACCGCC
AATGGCCTAAATTTCATCGATAAATGCAATTAA					