Detailed information of component protein
Summary
Component ID | T6CP003918 |
Component protein type | TssM |
T6SS ID (Type) | T6SS00382 (Type i3) |
Strain | Xenorhabdus bovienii SS-2004 |
Replicon | chromosome |
Sequence | Protein sequence (1172 a.a.); Nucleotide sequence (3516 bp) |
Reference |
External database links
Locus tag (Gene) | XBJ1_RS08955 |
Coordinate (Strand) | 2065703..2069218 (-) |
NCBI ID | WP_012988557.1 |
RefSeq | NC_013892 |
Uniprot ID | D3V3B6_XENBS |
KEGG ID | xbo:XBJ1_2105 |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related_N | PF14331.8 | 4.7e-69 | 207..437 |
IcmF-related | PF06761.14 | 2e-68 | 490..794 |
IcmF_C | PF06744.14 | 1.1e-21 | 1046..1150 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Inside | 1-12 | MLSAIVAIFTSR |
Transmembrane helix | 13-32 | LMWSFLGITAISSIIWFIGP |
Outside | 33-46 | MTSIGNTIPLGSST |
Transmembrane helix | 47-69 | VRIIIIAILYFVWLFIQLASHLY |
Inside | 70-428 | QVWLNKKLSAPLNRGNDEGEQVRNSQYSPLSEHFSDALRLLKNAYFYGSEIQYKPKWKPFFDRRYRYQLPWYVLIGPPNSGKTTLLANSGLHFPLSDHFETLSSNEIKETDNCHWWFTNQAVLLDTPGRYTTQDTRHEQDAREWKCFVNLLKKYRTRQPLNGVILTISVADLLNPSKKVRDQHAYLLRRRLSELQAQFKIQFPIYVIITKIDLLKGFGAYFAHFDKTQRDQIWGFNFPWNKTGWDLKDIFAQQYGLLQTRLNAELSHILLHLNDLQQCAESYLFPQEFAALQPLIAQHLEIAFAKSGFDIPYSPRGLYFTSSVQEEPIHQGYFLKTLLETLFQEAGLAGQNRWWVYRNR |
Transmembrane helix | 429-451 | LLKGLGYIILAAIMGLIITLFFS |
Outside | 452-1171 | SYGNNKNYLTEVQNKIFPILSQSAALKKISNDIDIYALLPILNKVADLGKSKYFSPDAPPWSYQMGLYHGEQINDASLTLYTKTLQTLLLPQVARLITSQLHQTNSDNIYNTYNTLKAYQMLCQPQHYDGKFLHRWVMQYLQAHLPPTISPTQLHQIEGHLRQLLEYQIVTFPYVCDNPLIKQKQILLSKIPLAQLVYIHLKQELSDNPNLSPVGLTTLAGPEAGLVFSRINGTPITLNIAGMFTPAGYQMGIGNNLTTLIDKLYSQNAWILGQYAEISTPRELTLLVRQFYLHDYLEQWDKFLFDIRLNNIDSLEQRSNIAQFLASNASPMRNLLINISKNVNLDERLSDNHFDKKIGELVSRKADSLTKVVPNPFMSGDNLPTPEQELRKHFAQITALANNPDGKNKQIPFDHVLSQLAKLHEYLDSVQNATNIGIPAPSDEIITQLQATAERLPIPFKGIISSLAMGANSDTQASNVKNLNKQLNTEVQGFCHQAIANRYPLTPWALDDIRPDDMARMFAPQTGIMDSFFQKNLVGKVDTSHENWRLILGTDSKAKALPESHKLLLPFQQAQIIRDTLFSDGSRIPSFRVIVRTVNMDKEILSMILDVDGQQLQYSHGPQVSQSLNWPGPNGSNQARIQLNLDDGTTASLITSGPWALNRLIDHEQFAAGKPATNNHTSQRVTFNIRGHHVSLEFIPNSILSPFQLPIFVCPKLINS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9814 | - | - |
Protein sequence: 1172 a.a.
>T6CP003918 NC_013892:c2069218-2065703 [Xenorhabdus bovienii SS-2004] [TssM]
MLSAIVAIFTSRLMWSFLGITAISSIIWFIGPMTSIGNTIPLGSSTVRIIIIAILYFVWLFIQLASHLYQVWLNKKLSAP
LNRGNDEGEQVRNSQYSPLSEHFSDALRLLKNAYFYGSEIQYKPKWKPFFDRRYRYQLPWYVLIGPPNSGKTTLLANSGL
HFPLSDHFETLSSNEIKETDNCHWWFTNQAVLLDTPGRYTTQDTRHEQDAREWKCFVNLLKKYRTRQPLNGVILTISVAD
LLNPSKKVRDQHAYLLRRRLSELQAQFKIQFPIYVIITKIDLLKGFGAYFAHFDKTQRDQIWGFNFPWNKTGWDLKDIFA
QQYGLLQTRLNAELSHILLHLNDLQQCAESYLFPQEFAALQPLIAQHLEIAFAKSGFDIPYSPRGLYFTSSVQEEPIHQG
YFLKTLLETLFQEAGLAGQNRWWVYRNRLLKGLGYIILAAIMGLIITLFFSSYGNNKNYLTEVQNKIFPILSQSAALKKI
SNDIDIYALLPILNKVADLGKSKYFSPDAPPWSYQMGLYHGEQINDASLTLYTKTLQTLLLPQVARLITSQLHQTNSDNI
YNTYNTLKAYQMLCQPQHYDGKFLHRWVMQYLQAHLPPTISPTQLHQIEGHLRQLLEYQIVTFPYVCDNPLIKQKQILLS
KIPLAQLVYIHLKQELSDNPNLSPVGLTTLAGPEAGLVFSRINGTPITLNIAGMFTPAGYQMGIGNNLTTLIDKLYSQNA
WILGQYAEISTPRELTLLVRQFYLHDYLEQWDKFLFDIRLNNIDSLEQRSNIAQFLASNASPMRNLLINISKNVNLDERL
SDNHFDKKIGELVSRKADSLTKVVPNPFMSGDNLPTPEQELRKHFAQITALANNPDGKNKQIPFDHVLSQLAKLHEYLDS
VQNATNIGIPAPSDEIITQLQATAERLPIPFKGIISSLAMGANSDTQASNVKNLNKQLNTEVQGFCHQAIANRYPLTPWA
LDDIRPDDMARMFAPQTGIMDSFFQKNLVGKVDTSHENWRLILGTDSKAKALPESHKLLLPFQQAQIIRDTLFSDGSRIP
SFRVIVRTVNMDKEILSMILDVDGQQLQYSHGPQVSQSLNWPGPNGSNQARIQLNLDDGTTASLITSGPWALNRLIDHEQ
FAAGKPATNNHTSQRVTFNIRGHHVSLEFIPNSILSPFQLPIFVCPKLINS*
MLSAIVAIFTSRLMWSFLGITAISSIIWFIGPMTSIGNTIPLGSSTVRIIIIAILYFVWLFIQLASHLYQVWLNKKLSAP
LNRGNDEGEQVRNSQYSPLSEHFSDALRLLKNAYFYGSEIQYKPKWKPFFDRRYRYQLPWYVLIGPPNSGKTTLLANSGL
HFPLSDHFETLSSNEIKETDNCHWWFTNQAVLLDTPGRYTTQDTRHEQDAREWKCFVNLLKKYRTRQPLNGVILTISVAD
LLNPSKKVRDQHAYLLRRRLSELQAQFKIQFPIYVIITKIDLLKGFGAYFAHFDKTQRDQIWGFNFPWNKTGWDLKDIFA
QQYGLLQTRLNAELSHILLHLNDLQQCAESYLFPQEFAALQPLIAQHLEIAFAKSGFDIPYSPRGLYFTSSVQEEPIHQG
YFLKTLLETLFQEAGLAGQNRWWVYRNRLLKGLGYIILAAIMGLIITLFFSSYGNNKNYLTEVQNKIFPILSQSAALKKI
SNDIDIYALLPILNKVADLGKSKYFSPDAPPWSYQMGLYHGEQINDASLTLYTKTLQTLLLPQVARLITSQLHQTNSDNI
YNTYNTLKAYQMLCQPQHYDGKFLHRWVMQYLQAHLPPTISPTQLHQIEGHLRQLLEYQIVTFPYVCDNPLIKQKQILLS
KIPLAQLVYIHLKQELSDNPNLSPVGLTTLAGPEAGLVFSRINGTPITLNIAGMFTPAGYQMGIGNNLTTLIDKLYSQNA
WILGQYAEISTPRELTLLVRQFYLHDYLEQWDKFLFDIRLNNIDSLEQRSNIAQFLASNASPMRNLLINISKNVNLDERL
SDNHFDKKIGELVSRKADSLTKVVPNPFMSGDNLPTPEQELRKHFAQITALANNPDGKNKQIPFDHVLSQLAKLHEYLDS
VQNATNIGIPAPSDEIITQLQATAERLPIPFKGIISSLAMGANSDTQASNVKNLNKQLNTEVQGFCHQAIANRYPLTPWA
LDDIRPDDMARMFAPQTGIMDSFFQKNLVGKVDTSHENWRLILGTDSKAKALPESHKLLLPFQQAQIIRDTLFSDGSRIP
SFRVIVRTVNMDKEILSMILDVDGQQLQYSHGPQVSQSLNWPGPNGSNQARIQLNLDDGTTASLITSGPWALNRLIDHEQ
FAAGKPATNNHTSQRVTFNIRGHHVSLEFIPNSILSPFQLPIFVCPKLINS*
Nucleotide sequence: 3516 bp
>T6CP003918 NC_013892:c2069218-2065703 [Xenorhabdus bovienii SS-2004] [TssM]
ATGTTGAGCGCCATTGTTGCTATTTTTACCAGCCGATTAATGTGGAGTTTCCTCGGCATCACCGCCATCTCTTCCATTAT
CTGGTTTATTGGTCCCATGACTTCTATCGGTAATACCATACCTCTTGGATCAAGTACGGTACGAATAATTATCATTGCCA
TATTATATTTTGTCTGGCTCTTTATCCAGTTAGCGTCTCACTTATATCAGGTTTGGCTGAATAAAAAACTTTCTGCCCCA
TTAAACAGAGGCAATGACGAGGGTGAACAAGTACGAAATTCACAATATTCCCCTTTATCAGAACACTTTTCTGATGCCTT
ACGACTACTGAAAAATGCCTATTTTTATGGTTCAGAGATCCAATATAAGCCAAAATGGAAACCTTTTTTCGATCGTCGAT
ATCGGTATCAGTTACCTTGGTATGTCCTGATTGGCCCCCCCAATTCAGGGAAAACAACCCTTCTGGCAAATTCTGGTTTG
CACTTTCCATTATCCGATCATTTCGAAACGCTTTCCTCAAACGAGATAAAAGAAACCGATAATTGTCACTGGTGGTTCAC
CAATCAGGCTGTCTTACTGGATACACCCGGACGATATACCACTCAGGATACACGGCACGAACAAGATGCCCGTGAATGGA
AATGTTTTGTCAATTTGCTGAAAAAATATCGCACACGCCAGCCCCTAAATGGCGTCATTCTTACCATTAGCGTAGCGGAT
TTATTAAACCCCTCAAAGAAAGTACGTGATCAACACGCTTATTTATTACGCAGGCGATTGTCAGAATTACAGGCTCAGTT
CAAGATCCAATTTCCAATCTATGTCATCATTACCAAGATTGATTTACTGAAAGGATTTGGGGCTTATTTTGCCCATTTTG
ACAAAACACAGCGCGATCAAATCTGGGGATTCAATTTCCCGTGGAACAAAACAGGCTGGGATCTGAAGGACATTTTTGCA
CAGCAATATGGGTTGTTGCAGACACGATTAAATGCAGAGCTATCCCATATTTTATTGCACCTGAATGATCTTCAGCAATG
TGCTGAAAGTTACCTGTTTCCGCAAGAATTTGCCGCACTGCAACCCTTAATTGCTCAGCATCTTGAAATTGCATTCGCTA
AATCAGGGTTTGATATTCCTTATTCACCACGTGGGCTTTATTTCACTAGCAGCGTACAGGAAGAGCCTATTCATCAAGGA
TATTTTCTGAAAACTCTGCTGGAAACGCTCTTTCAGGAAGCGGGGCTTGCCGGTCAAAATCGTTGGTGGGTGTACAGAAA
CCGTCTATTAAAAGGGCTGGGGTATATTATTCTGGCTGCGATCATGGGGTTAATCATCACCTTGTTTTTTAGCAGTTATG
GCAACAATAAAAATTACCTGACAGAAGTTCAAAATAAAATTTTCCCTATTCTGAGCCAAAGTGCAGCATTAAAAAAGATC
AGCAACGACATCGATATTTATGCCTTACTGCCTATTTTGAATAAAGTGGCGGATTTGGGAAAAAGTAAATATTTTTCTCC
TGATGCTCCCCCTTGGTCATACCAAATGGGCTTGTATCATGGTGAACAGATCAATGATGCCAGTTTGACTCTGTACACGA
AAACATTACAAACCTTATTGCTACCGCAAGTCGCACGGTTAATAACGTCTCAATTGCACCAAACCAACAGCGATAATATA
TATAACACTTACAATACGTTGAAAGCGTATCAAATGTTATGTCAGCCCCAACATTATGACGGTAAGTTCTTACATCGTTG
GGTGATGCAATATTTGCAGGCTCATTTACCCCCCACTATTTCACCGACACAATTACACCAAATTGAGGGGCATCTGCGCC
AATTATTGGAATATCAGATCGTTACTTTCCCTTATGTTTGTGATAACCCACTGATAAAACAAAAACAGATTTTACTTAGC
AAGATCCCACTAGCACAACTCGTTTATATCCATTTAAAACAGGAACTATCGGATAACCCCAATTTATCGCCAGTCGGCTT
AACAACGCTTGCTGGCCCAGAAGCCGGATTGGTTTTCTCACGCATCAACGGTACACCGATTACCCTTAATATAGCGGGGA
TGTTTACTCCGGCGGGGTATCAAATGGGGATAGGTAACAATCTCACTACCCTCATCGATAAGTTATACAGTCAAAATGCA
TGGATACTGGGCCAGTATGCAGAAATATCAACCCCAAGAGAGCTTACCCTCTTAGTACGACAATTTTATCTCCATGATTA
TCTTGAACAATGGGATAAATTTCTGTTCGACATCCGTCTAAACAATATTGACAGCCTGGAGCAGCGTTCTAATATAGCCC
AGTTTCTGGCTTCAAATGCTTCTCCCATGCGCAATCTGCTCATTAATATCAGTAAGAATGTCAATCTGGATGAAAGGTTA
AGCGATAACCATTTTGATAAAAAAATCGGTGAGTTGGTCAGCCGTAAAGCAGATTCATTGACAAAAGTCGTACCTAATCC
ATTCATGTCAGGAGATAACCTCCCCACCCCGGAACAAGAATTGAGAAAACATTTTGCCCAAATTACTGCATTGGCAAACA
ATCCAGACGGTAAAAATAAGCAAATTCCTTTTGATCATGTTCTCAGTCAACTTGCCAAATTACACGAATATCTGGATTCA
GTACAAAATGCTACCAATATAGGCATACCCGCGCCATCGGATGAAATTATCACCCAACTCCAAGCCACGGCGGAGCGGTT
GCCGATCCCATTCAAGGGCATCATTTCCTCGTTGGCAATGGGGGCTAACAGTGATACACAAGCCAGTAACGTAAAGAATC
TCAATAAGCAGCTGAATACAGAAGTCCAAGGCTTTTGTCATCAAGCGATTGCCAACCGCTATCCGCTGACGCCGTGGGCA
CTTGATGATATCAGACCGGATGATATGGCTCGGATGTTTGCGCCACAAACGGGGATCATGGATAGCTTTTTCCAAAAAAA
TCTGGTGGGAAAAGTCGATACATCTCACGAAAACTGGCGCCTTATACTGGGAACGGATAGCAAAGCAAAAGCATTACCGG
AAAGTCACAAGTTACTCCTGCCATTCCAACAAGCCCAGATCATTCGAGATACTTTATTCAGCGACGGCTCGCGTATCCCT
TCATTTCGGGTCATCGTCCGCACCGTAAATATGGATAAAGAGATATTGAGTATGATACTGGATGTTGATGGTCAGCAATT
GCAATATAGCCACGGCCCACAAGTTTCGCAGTCACTCAATTGGCCTGGCCCAAACGGCTCCAATCAGGCTCGTATCCAAT
TAAATTTAGATGATGGGACAACAGCGAGTCTCATCACTTCCGGCCCGTGGGCATTAAATCGCTTAATTGACCATGAACAA
TTCGCGGCAGGAAAACCCGCCACAAATAATCATACAAGCCAGCGTGTGACATTTAATATCAGAGGCCATCATGTCTCATT
GGAATTTATTCCAAACAGTATTCTCAGCCCGTTTCAACTTCCCATTTTCGTCTGTCCCAAGCTGATTAATTCATAA
ATGTTGAGCGCCATTGTTGCTATTTTTACCAGCCGATTAATGTGGAGTTTCCTCGGCATCACCGCCATCTCTTCCATTAT
CTGGTTTATTGGTCCCATGACTTCTATCGGTAATACCATACCTCTTGGATCAAGTACGGTACGAATAATTATCATTGCCA
TATTATATTTTGTCTGGCTCTTTATCCAGTTAGCGTCTCACTTATATCAGGTTTGGCTGAATAAAAAACTTTCTGCCCCA
TTAAACAGAGGCAATGACGAGGGTGAACAAGTACGAAATTCACAATATTCCCCTTTATCAGAACACTTTTCTGATGCCTT
ACGACTACTGAAAAATGCCTATTTTTATGGTTCAGAGATCCAATATAAGCCAAAATGGAAACCTTTTTTCGATCGTCGAT
ATCGGTATCAGTTACCTTGGTATGTCCTGATTGGCCCCCCCAATTCAGGGAAAACAACCCTTCTGGCAAATTCTGGTTTG
CACTTTCCATTATCCGATCATTTCGAAACGCTTTCCTCAAACGAGATAAAAGAAACCGATAATTGTCACTGGTGGTTCAC
CAATCAGGCTGTCTTACTGGATACACCCGGACGATATACCACTCAGGATACACGGCACGAACAAGATGCCCGTGAATGGA
AATGTTTTGTCAATTTGCTGAAAAAATATCGCACACGCCAGCCCCTAAATGGCGTCATTCTTACCATTAGCGTAGCGGAT
TTATTAAACCCCTCAAAGAAAGTACGTGATCAACACGCTTATTTATTACGCAGGCGATTGTCAGAATTACAGGCTCAGTT
CAAGATCCAATTTCCAATCTATGTCATCATTACCAAGATTGATTTACTGAAAGGATTTGGGGCTTATTTTGCCCATTTTG
ACAAAACACAGCGCGATCAAATCTGGGGATTCAATTTCCCGTGGAACAAAACAGGCTGGGATCTGAAGGACATTTTTGCA
CAGCAATATGGGTTGTTGCAGACACGATTAAATGCAGAGCTATCCCATATTTTATTGCACCTGAATGATCTTCAGCAATG
TGCTGAAAGTTACCTGTTTCCGCAAGAATTTGCCGCACTGCAACCCTTAATTGCTCAGCATCTTGAAATTGCATTCGCTA
AATCAGGGTTTGATATTCCTTATTCACCACGTGGGCTTTATTTCACTAGCAGCGTACAGGAAGAGCCTATTCATCAAGGA
TATTTTCTGAAAACTCTGCTGGAAACGCTCTTTCAGGAAGCGGGGCTTGCCGGTCAAAATCGTTGGTGGGTGTACAGAAA
CCGTCTATTAAAAGGGCTGGGGTATATTATTCTGGCTGCGATCATGGGGTTAATCATCACCTTGTTTTTTAGCAGTTATG
GCAACAATAAAAATTACCTGACAGAAGTTCAAAATAAAATTTTCCCTATTCTGAGCCAAAGTGCAGCATTAAAAAAGATC
AGCAACGACATCGATATTTATGCCTTACTGCCTATTTTGAATAAAGTGGCGGATTTGGGAAAAAGTAAATATTTTTCTCC
TGATGCTCCCCCTTGGTCATACCAAATGGGCTTGTATCATGGTGAACAGATCAATGATGCCAGTTTGACTCTGTACACGA
AAACATTACAAACCTTATTGCTACCGCAAGTCGCACGGTTAATAACGTCTCAATTGCACCAAACCAACAGCGATAATATA
TATAACACTTACAATACGTTGAAAGCGTATCAAATGTTATGTCAGCCCCAACATTATGACGGTAAGTTCTTACATCGTTG
GGTGATGCAATATTTGCAGGCTCATTTACCCCCCACTATTTCACCGACACAATTACACCAAATTGAGGGGCATCTGCGCC
AATTATTGGAATATCAGATCGTTACTTTCCCTTATGTTTGTGATAACCCACTGATAAAACAAAAACAGATTTTACTTAGC
AAGATCCCACTAGCACAACTCGTTTATATCCATTTAAAACAGGAACTATCGGATAACCCCAATTTATCGCCAGTCGGCTT
AACAACGCTTGCTGGCCCAGAAGCCGGATTGGTTTTCTCACGCATCAACGGTACACCGATTACCCTTAATATAGCGGGGA
TGTTTACTCCGGCGGGGTATCAAATGGGGATAGGTAACAATCTCACTACCCTCATCGATAAGTTATACAGTCAAAATGCA
TGGATACTGGGCCAGTATGCAGAAATATCAACCCCAAGAGAGCTTACCCTCTTAGTACGACAATTTTATCTCCATGATTA
TCTTGAACAATGGGATAAATTTCTGTTCGACATCCGTCTAAACAATATTGACAGCCTGGAGCAGCGTTCTAATATAGCCC
AGTTTCTGGCTTCAAATGCTTCTCCCATGCGCAATCTGCTCATTAATATCAGTAAGAATGTCAATCTGGATGAAAGGTTA
AGCGATAACCATTTTGATAAAAAAATCGGTGAGTTGGTCAGCCGTAAAGCAGATTCATTGACAAAAGTCGTACCTAATCC
ATTCATGTCAGGAGATAACCTCCCCACCCCGGAACAAGAATTGAGAAAACATTTTGCCCAAATTACTGCATTGGCAAACA
ATCCAGACGGTAAAAATAAGCAAATTCCTTTTGATCATGTTCTCAGTCAACTTGCCAAATTACACGAATATCTGGATTCA
GTACAAAATGCTACCAATATAGGCATACCCGCGCCATCGGATGAAATTATCACCCAACTCCAAGCCACGGCGGAGCGGTT
GCCGATCCCATTCAAGGGCATCATTTCCTCGTTGGCAATGGGGGCTAACAGTGATACACAAGCCAGTAACGTAAAGAATC
TCAATAAGCAGCTGAATACAGAAGTCCAAGGCTTTTGTCATCAAGCGATTGCCAACCGCTATCCGCTGACGCCGTGGGCA
CTTGATGATATCAGACCGGATGATATGGCTCGGATGTTTGCGCCACAAACGGGGATCATGGATAGCTTTTTCCAAAAAAA
TCTGGTGGGAAAAGTCGATACATCTCACGAAAACTGGCGCCTTATACTGGGAACGGATAGCAAAGCAAAAGCATTACCGG
AAAGTCACAAGTTACTCCTGCCATTCCAACAAGCCCAGATCATTCGAGATACTTTATTCAGCGACGGCTCGCGTATCCCT
TCATTTCGGGTCATCGTCCGCACCGTAAATATGGATAAAGAGATATTGAGTATGATACTGGATGTTGATGGTCAGCAATT
GCAATATAGCCACGGCCCACAAGTTTCGCAGTCACTCAATTGGCCTGGCCCAAACGGCTCCAATCAGGCTCGTATCCAAT
TAAATTTAGATGATGGGACAACAGCGAGTCTCATCACTTCCGGCCCGTGGGCATTAAATCGCTTAATTGACCATGAACAA
TTCGCGGCAGGAAAACCCGCCACAAATAATCATACAAGCCAGCGTGTGACATTTAATATCAGAGGCCATCATGTCTCATT
GGAATTTATTCCAAACAGTATTCTCAGCCCGTTTCAACTTCCCATTTTCGTCTGTCCCAAGCTGATTAATTCATAA