SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003917
Component protein type	TssA
T6SS ID (Type)	T6SS00382 (Type i3)
Strain	Xenorhabdus bovienii SS-2004
Replicon	chromosome
Sequence	Protein sequence (352 a.a.); Nucleotide sequence (1056 bp)
Reference	[1] Kochanowsky RM, Bradshaw C, Forlastro I, et al. Xenorhabdus bovienii strain jolietti uses a type 6 secretion system to kill closely related Xenorhabdus strains. FEMS Microbiol Ecol. 2020 Aug 1;96(8). pii: fiaa073. doi: 10.1093/femsec/fiaa073. PMID: 32558899

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	XBJ1_RS08950
Coordinate (Strand)	2064485..2065540 (-)
NCBI ID	WP_012988556.1
RefSeq	NC_013892
Uniprot ID	D3V3B5_XENBS
KEGG ID	xbo:XBJ1_2104
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.2e-33	7..130

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-351	MNIETLLEPVSIESPCGENLEYDPEFMALEQSLLGKTEQQFGDITIPAETPDWIQAEKQAISLLRRTKDLRIIMALIQSWLQMRGLCGYADGLNLLQQTLERYWDEVWPKLAFEGEYDPQFRLNILAAIEDDSPLSLQAQYSTLLRNTSKELSLLEVCSLLDGTINEISGYTGGRTRLQEELKQQASSPEVLAVIRIRDHLIAILDIIRQAFSESYVSELSWLLKQLNKVIEFCPSLSKTRHRDKETDPKNIFDSTTCSEQRTPASTYSTHAVSASWHTIEASNRDEARMLLEKAKNYFVVNEPSHPAPHMIARIQRLIDRDFVDIIYDLAPDGLNQLEIIFGRSDNFDSN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9920	-	-

Protein sequence: 352 a.a.

						>T6CP003917 NC_013892:c2065540-2064485 [Xenorhabdus bovienii SS-2004] [TssA]
MNIETLLEPVSIESPCGENLEYDPEFMALEQSLLGKTEQQFGDITIPAETPDWIQAEKQAISLLRRTKDLRIIMALIQSW
LQMRGLCGYADGLNLLQQTLERYWDEVWPKLAFEGEYDPQFRLNILAAIEDDSPLSLQAQYSTLLRNTSKELSLLEVCSL
LDGTINEISGYTGGRTRLQEELKQQASSPEVLAVIRIRDHLIAILDIIRQAFSESYVSELSWLLKQLNKVIEFCPSLSKT
RHRDKETDPKNIFDSTTCSEQRTPASTYSTHAVSASWHTIEASNRDEARMLLEKAKNYFVVNEPSHPAPHMIARIQRLID
RDFVDIIYDLAPDGLNQLEIIFGRSDNFDSN*					

Nucleotide sequence: 1056 bp

						>T6CP003917 NC_013892:c2065540-2064485 [Xenorhabdus bovienii SS-2004] [TssA]
ATGAACATCGAAACCTTACTCGAACCCGTCAGCATCGAATCTCCTTGTGGGGAAAATCTGGAATACGATCCTGAATTTAT
GGCATTAGAGCAATCCTTGCTTGGAAAAACGGAACAGCAGTTTGGTGATATCACTATTCCAGCGGAGACACCTGACTGGA
TACAGGCCGAAAAACAAGCCATTAGCCTACTGAGGCGGACCAAAGATCTGCGCATCATCATGGCACTGATACAATCATGG
TTGCAAATGCGGGGATTATGTGGCTACGCAGACGGCCTTAATCTGCTCCAGCAGACACTGGAACGTTATTGGGATGAAGT
TTGGCCGAAATTAGCGTTTGAGGGGGAATATGATCCCCAATTTCGCCTCAACATACTTGCCGCTATTGAGGACGATTCTC
CTCTTTCGTTGCAAGCGCAATATTCCACTCTGTTGCGGAACACGTCAAAGGAGTTGTCATTGCTGGAGGTTTGCTCATTA
TTGGATGGCACGATAAACGAAATCAGCGGCTATACTGGCGGGCGAACCCGATTACAGGAGGAGTTAAAGCAGCAGGCCAG
TAGCCCTGAAGTTTTGGCCGTGATCCGTATTCGGGATCACCTCATTGCAATACTCGACATTATTCGTCAGGCATTCTCAG
AAAGTTATGTTTCTGAATTGTCTTGGTTATTAAAACAATTAAATAAAGTGATTGAATTTTGCCCTTCCCTTTCAAAAACA
AGGCATCGCGATAAGGAGACAGATCCCAAAAATATTTTTGACTCAACAACATGTTCGGAACAGAGAACGCCTGCATCTAC
ATATTCAACCCATGCGGTATCTGCTTCTTGGCATACCATTGAAGCCAGCAACCGTGATGAAGCACGCATGTTACTAGAAA
AGGCTAAAAACTATTTTGTCGTCAATGAACCCAGTCACCCTGCCCCACATATGATTGCCCGTATTCAGCGGTTGATTGAT
CGTGATTTCGTTGACATTATTTATGATCTTGCCCCAGATGGACTCAACCAGCTCGAAATCATTTTTGGTCGCTCAGACAA
TTTTGACTCGAATTAA					