Detailed information of component protein
Summary
| Component ID | T6CP003905 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00381  (Type i1) |
| Strain | Xenorhabdus bovienii SS-2004 |
| Replicon | chromosome |
| Sequence | Protein sequence (185 a.a.); Nucleotide sequence (555 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | XBJ1_RS01180 |
| Coordinate (Strand) | 276634..277188 (+) |
| NCBI ID | WP_012986882.1 |
| RefSeq | NC_013892 |
| Uniprot ID | A0A077P679_XENBV, D3UYP2_XENBS |
| KEGG ID | xbo:XBJ1_0269 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.5e-31 | 56..175 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-8 | MKRSASHR |
| Transmembrane helix | 9-31 | VWAVGILLLSAMLLNGCSGAWNA |
| Outside | 32-184 | TKKVGQVIWDPSTPVGKPDEQASVANITLLAEPNINPNESGEAAPVEMNLVYLSEDSRFLAADYDQLESDKLEKALGKNYIDHQDYTLLPGQYKPLSTLILEKNNRYIGVIVHYADANQSEWKKIIRVKDIGRHYNILVHVRNNDVELRKEEE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9899 | pos: 24-25. LNG-CS. | MKRSASHRVWAVGILLLSAMLLNG |
Protein sequence: 185 a.a.
>T6CP003905 NC_013892:276634-277188 [Xenorhabdus bovienii SS-2004] [TssJ]
MKRSASHRVWAVGILLLSAMLLNGCSGAWNATKKVGQVIWDPSTPVGKPDEQASVANITLLAEPNINPNESGEAAPVEMN
LVYLSEDSRFLAADYDQLESDKLEKALGKNYIDHQDYTLLPGQYKPLSTLILEKNNRYIGVIVHYADANQSEWKKIIRVK
DIGRHYNILVHVRNNDVELRKEEE*
MKRSASHRVWAVGILLLSAMLLNGCSGAWNATKKVGQVIWDPSTPVGKPDEQASVANITLLAEPNINPNESGEAAPVEMN
LVYLSEDSRFLAADYDQLESDKLEKALGKNYIDHQDYTLLPGQYKPLSTLILEKNNRYIGVIVHYADANQSEWKKIIRVK
DIGRHYNILVHVRNNDVELRKEEE*
Nucleotide sequence: 555 bp
>T6CP003905 NC_013892:276634-277188 [Xenorhabdus bovienii SS-2004] [TssJ]
ATGAAACGTTCCGCTAGTCATCGTGTATGGGCTGTAGGGATTTTATTGTTGTCAGCGATGTTGCTGAATGGCTGTAGCGG
TGCATGGAACGCGACAAAGAAAGTCGGTCAAGTGATCTGGGATCCTTCCACACCAGTAGGAAAACCGGATGAACAGGCTT
CTGTTGCCAACATTACGTTATTGGCCGAACCGAATATCAACCCCAATGAAAGTGGTGAAGCGGCTCCTGTTGAAATGAAT
CTGGTTTATCTCAGTGAAGATTCTCGTTTTTTGGCTGCTGATTATGACCAGCTTGAAAGCGACAAACTCGAAAAAGCACT
GGGGAAAAATTATATCGATCATCAGGATTACACACTATTACCGGGGCAATACAAGCCCCTGTCAACGCTGATATTGGAAA
AGAATAACCGTTACATCGGTGTCATCGTTCACTATGCAGATGCAAATCAATCTGAATGGAAAAAGATCATCCGAGTAAAA
GACATCGGCCGCCATTACAACATCTTGGTGCATGTCAGAAATAACGACGTCGAACTTAGAAAAGAGGAGGAGTAG
ATGAAACGTTCCGCTAGTCATCGTGTATGGGCTGTAGGGATTTTATTGTTGTCAGCGATGTTGCTGAATGGCTGTAGCGG
TGCATGGAACGCGACAAAGAAAGTCGGTCAAGTGATCTGGGATCCTTCCACACCAGTAGGAAAACCGGATGAACAGGCTT
CTGTTGCCAACATTACGTTATTGGCCGAACCGAATATCAACCCCAATGAAAGTGGTGAAGCGGCTCCTGTTGAAATGAAT
CTGGTTTATCTCAGTGAAGATTCTCGTTTTTTGGCTGCTGATTATGACCAGCTTGAAAGCGACAAACTCGAAAAAGCACT
GGGGAAAAATTATATCGATCATCAGGATTACACACTATTACCGGGGCAATACAAGCCCCTGTCAACGCTGATATTGGAAA
AGAATAACCGTTACATCGGTGTCATCGTTCACTATGCAGATGCAAATCAATCTGAATGGAAAAAGATCATCCGAGTAAAA
GACATCGGCCGCCATTACAACATCTTGGTGCATGTCAGAAATAACGACGTCGAACTTAGAAAAGAGGAGGAGTAG