Detailed information of component protein


Summary

Component ID T6CP003518
Component protein type TssM
T6SS ID (Type) T6SS00346 experimental (Type i4b)
Strain Edwardsiella ictaluri 93-146
Replicon chromosome
Sequence Protein sequence (1261 a.a.); Nucleotide sequence (3783 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) NT01EI_RS11960
Coordinate (Strand) 2647935..2651717 (+)
NCBI ID WP_015872020.1
RefSeq NC_012779
Uniprot ID C5BAE9_EDWI9
KEGG ID eic:NT01EI_2751
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.3e-51 172..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKRIFK
Transmembrane helix 7-26 WIAIALILIMLAMLCWLLTF
Outside 27-29 WQR
Transmembrane helix 30-52 WPAAAAWVLFLAILAGALLTRYI
Inside 53-417 WRKVRAWRTRSLEAEPVKKNSATERHPVTLQRQWRAATTLLRHSQLRRFRHPLQVLPWFMMLGRPGSGKSALLTQSGLAAPLDLPHEMHDGKAPVGWWYFDKAVILDISGQFVQPEASQDTRTTWNRMLDLLGRDRAQAGLSGLVLTVSAQTLLSANGEDLIDEGRLIRTRIEQLIRLFDRRFPVYVLVTQIDSLYGMEAWSHLLPQEVQEQAMGHIVDEPEIGHDVRDVIDIVLSKVNSRLKTMQLAMILRHPDADPALLMFPTELARLAEPLKRYVQHALGDSPYLESPILRGAFFTSAVQEGNTRSGSRDQQEIALQEAAHDTVKQGIFVRDLFNRVLPADRDLSRPVAVTSPWRRLNRHLG
Transmembrane helix 418-440 LFAWWLCVAAALGYLGVSAVYWQ
Outside 441-1260 RTLDDLRQRYPETMIMNNTLPHDMRIMIAQRDAIYWLEKRNAQWASRVLAFQHQLQYVEADAKSQYVNDFSKYILPSLDKAIRQSTTEENITSHPDGLVNAFQIEARRANLLRARLRGSSYGALMAMPPIPTPSLQLLYPTLDSNTVSRFNDLFLSQLAWSSDLQLQNSRLLHLQDRLRQLAAQSATLDWVIPWANAQPDLASVTLTDFWQGSLRPNKLPEVAAAYTLNGERRIHSFLSEIRNSMPNDSAFDKKLTVFRQEYAQKRLQAWQDFVGQFSQGEQILAGQGEWQSTIGRLPHDDNPYLRLARRLLSEFADMPDDLRPGWLRVLPQWLEVRQRAHQQAIVRVPGLVSDMGSMILRGAAQKGGLDQPRSQLVSRLDAIRLYQEYQAAITQAMNSVVTSQAQATKVAADFHGFDSQQNGQSPLQTAYARLISLRSLLGHNRPDEQLTWGLMAGSLQLVIRYADQQASCFLQNAWQDNVIAPSRWTLNQADRADKLYGENGVIWTFLSGPIKPFIQRNDSGWQVVDTLGQSVPFTADFLPFINQALIRQVDLKAGKIEQHQKQQLATLAQQQQKQQWQTLAAESAEKLAELNTTANTLKANSYPVSLMGLPTDVNSDARAKVVGTLLTMQCSNATLRLNNLNFAVMQSIQWSQHQCGQVTLQIRLPAFTLTRSYPGPRGFVNFLRDFPGGARRFTPADFPADKNRMAAVGITHITVRYRISGQETLLQDAAQLIQTESDIQSAQSRQQQAQQALITLNQPTAPEIPPAVDQLPVLNVMGVPERIGQCWRGDVRLARENGSIGQLMSELVAKQLTDKP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9926 - -


  Protein sequence: 1261 a.a.    

>T6CP003518 NC_012779:2647935-2651717 [Edwardsiella ictaluri 93-146] [TssM]
MKRIFKWIAIALILIMLAMLCWLLTFWQRWPAAAAWVLFLAILAGALLTRYIWRKVRAWRTRSLEAEPVKKNSATERHPV
TLQRQWRAATTLLRHSQLRRFRHPLQVLPWFMMLGRPGSGKSALLTQSGLAAPLDLPHEMHDGKAPVGWWYFDKAVILDI
SGQFVQPEASQDTRTTWNRMLDLLGRDRAQAGLSGLVLTVSAQTLLSANGEDLIDEGRLIRTRIEQLIRLFDRRFPVYVL
VTQIDSLYGMEAWSHLLPQEVQEQAMGHIVDEPEIGHDVRDVIDIVLSKVNSRLKTMQLAMILRHPDADPALLMFPTELA
RLAEPLKRYVQHALGDSPYLESPILRGAFFTSAVQEGNTRSGSRDQQEIALQEAAHDTVKQGIFVRDLFNRVLPADRDLS
RPVAVTSPWRRLNRHLGLFAWWLCVAAALGYLGVSAVYWQRTLDDLRQRYPETMIMNNTLPHDMRIMIAQRDAIYWLEKR
NAQWASRVLAFQHQLQYVEADAKSQYVNDFSKYILPSLDKAIRQSTTEENITSHPDGLVNAFQIEARRANLLRARLRGSS
YGALMAMPPIPTPSLQLLYPTLDSNTVSRFNDLFLSQLAWSSDLQLQNSRLLHLQDRLRQLAAQSATLDWVIPWANAQPD
LASVTLTDFWQGSLRPNKLPEVAAAYTLNGERRIHSFLSEIRNSMPNDSAFDKKLTVFRQEYAQKRLQAWQDFVGQFSQG
EQILAGQGEWQSTIGRLPHDDNPYLRLARRLLSEFADMPDDLRPGWLRVLPQWLEVRQRAHQQAIVRVPGLVSDMGSMIL
RGAAQKGGLDQPRSQLVSRLDAIRLYQEYQAAITQAMNSVVTSQAQATKVAADFHGFDSQQNGQSPLQTAYARLISLRSL
LGHNRPDEQLTWGLMAGSLQLVIRYADQQASCFLQNAWQDNVIAPSRWTLNQADRADKLYGENGVIWTFLSGPIKPFIQR
NDSGWQVVDTLGQSVPFTADFLPFINQALIRQVDLKAGKIEQHQKQQLATLAQQQQKQQWQTLAAESAEKLAELNTTANT
LKANSYPVSLMGLPTDVNSDARAKVVGTLLTMQCSNATLRLNNLNFAVMQSIQWSQHQCGQVTLQIRLPAFTLTRSYPGP
RGFVNFLRDFPGGARRFTPADFPADKNRMAAVGITHITVRYRISGQETLLQDAAQLIQTESDIQSAQSRQQQAQQALITL
NQPTAPEIPPAVDQLPVLNVMGVPERIGQCWRGDVRLARENGSIGQLMSELVAKQLTDKP*

  Nucleotide sequence: 3783 bp    

>T6CP003518 NC_012779:2647935-2651717 [Edwardsiella ictaluri 93-146] [TssM]
ATGAAACGTATCTTCAAATGGATAGCGATTGCACTGATTCTGATCATGCTGGCGATGCTGTGCTGGCTGCTCACGTTTTG
GCAACGCTGGCCTGCCGCTGCCGCATGGGTACTGTTTCTCGCCATTCTGGCCGGCGCCCTGCTGACACGCTATATCTGGC
GTAAGGTACGGGCATGGCGTACCCGCAGCCTGGAGGCGGAGCCGGTTAAAAAAAACAGCGCCACGGAACGCCATCCCGTA
ACGCTACAGCGCCAGTGGAGAGCGGCCACAACCCTGTTGAGGCACTCCCAGCTGCGCCGTTTCCGCCACCCGCTGCAAGT
CCTACCCTGGTTTATGATGCTGGGCCGTCCAGGCTCAGGTAAATCGGCGCTGCTGACACAGTCAGGCCTGGCCGCACCGT
TGGATTTGCCCCATGAGATGCATGACGGTAAGGCGCCTGTCGGCTGGTGGTATTTCGACAAGGCCGTCATCCTGGATATC
TCCGGCCAGTTTGTACAGCCGGAGGCATCGCAGGATACCCGTACGACCTGGAACAGAATGCTGGATCTGCTGGGGCGCGA
TCGCGCCCAGGCCGGCTTGAGTGGGCTGGTGCTCACGGTGTCGGCCCAGACCCTGCTGTCAGCGAATGGTGAAGACCTGA
TCGATGAGGGGCGGCTAATCCGAACCCGGATAGAGCAACTGATCCGTCTGTTCGACAGACGCTTCCCCGTCTACGTACTG
GTAACCCAAATTGACAGCCTGTACGGCATGGAGGCCTGGTCCCACCTGCTGCCCCAAGAGGTACAGGAACAGGCCATGGG
TCACATTGTGGACGAACCGGAGATCGGTCACGATGTCCGTGATGTGATCGATATCGTATTGAGCAAGGTAAATAGCCGAC
TGAAAACCATGCAGCTGGCGATGATACTGCGGCACCCGGATGCCGATCCGGCCCTATTGATGTTCCCTACCGAGCTGGCC
AGACTGGCCGAGCCTCTCAAGCGCTATGTCCAGCATGCCCTGGGAGACAGCCCCTATCTGGAGTCACCCATACTGCGTGG
CGCCTTTTTCACCAGTGCGGTTCAGGAGGGGAACACCCGCAGCGGGTCCCGGGATCAGCAGGAGATTGCGCTGCAAGAGG
CGGCACATGATACCGTCAAACAAGGGATCTTTGTCCGGGATCTGTTCAACCGCGTTCTGCCGGCAGACCGTGACCTGAGC
CGCCCTGTTGCCGTGACCAGTCCCTGGCGGCGTCTGAACCGTCATCTGGGCCTGTTTGCCTGGTGGCTGTGTGTAGCCGC
AGCCCTTGGGTATCTCGGCGTATCGGCCGTATACTGGCAGCGTACGCTGGATGATCTGCGCCAGCGCTATCCGGAGACCA
TGATCATGAATAACACCCTGCCCCACGATATGCGGATTATGATTGCACAGCGGGATGCGATTTATTGGCTGGAAAAGAGA
AACGCGCAGTGGGCCAGCCGGGTACTGGCATTCCAGCATCAGCTTCAATATGTTGAAGCTGATGCAAAATCACAGTATGT
AAATGATTTTAGCAAATATATATTGCCCAGCCTGGATAAAGCCATTCGCCAGAGTACCACGGAGGAAAACATCACATCCC
ATCCGGACGGACTGGTGAATGCCTTTCAGATCGAAGCCCGCCGCGCGAACCTACTACGGGCGAGGCTGCGGGGCTCCAGC
TATGGAGCGTTGATGGCCATGCCCCCCATACCGACCCCGTCCCTACAGTTACTCTATCCGACCCTGGATTCGAATACGGT
ATCCCGTTTCAATGACCTGTTCCTGTCCCAACTTGCCTGGTCATCGGATCTCCAGCTGCAAAATTCCCGTCTGCTGCACC
TGCAAGACAGGCTCCGACAGCTGGCGGCACAAAGTGCCACCCTGGACTGGGTCATTCCATGGGCAAACGCCCAGCCTGAT
TTGGCAAGCGTAACCCTGACCGACTTCTGGCAGGGCTCACTGCGGCCGAATAAACTGCCGGAGGTCGCTGCGGCCTATAC
CCTGAATGGAGAGCGGCGTATCCACTCATTCCTGAGTGAGATACGCAACAGCATGCCGAACGACTCCGCCTTCGACAAAA
AGCTGACCGTATTCAGGCAGGAATATGCGCAGAAACGATTGCAGGCCTGGCAGGATTTCGTCGGCCAGTTTTCTCAGGGA
GAGCAGATCCTGGCCGGTCAGGGCGAGTGGCAGAGTACCATTGGCAGACTGCCCCATGATGACAACCCGTATCTGCGCCT
TGCCCGCCGTCTGTTGAGCGAATTCGCCGACATGCCGGATGACTTGCGCCCGGGCTGGCTCCGTGTGCTCCCGCAGTGGC
TGGAGGTACGCCAGCGCGCCCACCAGCAGGCCATCGTCAGGGTTCCCGGGCTGGTCAGTGATATGGGCAGCATGATCCTG
CGCGGGGCGGCGCAGAAGGGGGGACTGGATCAGCCCCGCAGCCAGCTCGTCAGCCGACTCGATGCCATTCGCCTGTATCA
GGAGTATCAGGCGGCCATAACCCAGGCAATGAACAGCGTCGTCACCAGTCAGGCTCAGGCCACCAAAGTGGCGGCGGACT
TCCATGGCTTTGACAGCCAGCAGAACGGACAGTCCCCATTGCAGACGGCCTATGCACGCCTGATAAGTCTACGCAGCCTG
CTGGGACACAATCGGCCGGACGAACAGCTCACCTGGGGGCTGATGGCTGGCTCCTTACAGCTGGTTATACGCTATGCCGA
TCAGCAAGCCTCCTGTTTCCTGCAGAACGCGTGGCAGGACAACGTCATCGCACCATCGCGCTGGACCCTTAATCAGGCCG
ATCGGGCGGATAAGCTGTATGGTGAGAATGGAGTGATCTGGACCTTTTTGTCCGGTCCAATTAAGCCGTTTATCCAGCGT
AACGATTCGGGCTGGCAGGTGGTGGATACCCTGGGACAATCAGTCCCCTTTACCGCAGACTTCCTGCCGTTTATCAATCA
GGCCCTGATTCGCCAGGTTGATCTGAAAGCTGGAAAAATCGAACAGCATCAGAAACAGCAGCTGGCCACCCTGGCCCAGC
AGCAGCAGAAGCAACAGTGGCAAACCCTTGCCGCAGAGTCTGCCGAAAAACTGGCCGAACTGAACACCACCGCCAATACC
CTGAAAGCGAATAGCTATCCCGTCTCCCTGATGGGACTGCCCACGGACGTCAACAGCGATGCCAGGGCCAAGGTCGTAGG
AACCCTGTTGACCATGCAGTGCTCCAATGCCACGCTCCGGCTAAACAACCTTAACTTTGCCGTAATGCAAAGTATACAGT
GGTCACAGCATCAGTGCGGCCAGGTGACGCTGCAGATCAGGCTCCCTGCCTTTACGCTGACGCGCAGCTATCCCGGGCCT
AGGGGCTTTGTCAACTTCTTACGGGATTTCCCGGGCGGAGCACGCCGTTTTACCCCGGCGGACTTCCCCGCTGACAAAAA
CCGGATGGCAGCGGTCGGGATAACCCATATTACGGTACGCTACCGTATCTCTGGGCAGGAGACCCTGTTGCAAGACGCCG
CTCAGCTGATACAGACAGAAAGTGATATTCAGTCAGCCCAGAGTCGCCAGCAGCAGGCACAGCAAGCCCTGATCACATTG
AATCAGCCGACCGCACCGGAGATCCCTCCGGCTGTGGATCAGCTGCCGGTGCTGAATGTGATGGGTGTACCGGAGCGCAT
CGGACAGTGCTGGCGGGGGGATGTTCGCCTTGCCAGAGAAAACGGCAGCATTGGTCAGTTAATGAGTGAGTTGGTCGCCA
AACAGTTAACCGACAAGCCCTAA