Detailed information of component protein
Summary
| Component ID | T6CP003516 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00346  (Type i4b) |
| Strain | Edwardsiella ictaluri 93-146 |
| Replicon | chromosome |
| Sequence | Protein sequence (210 a.a.); Nucleotide sequence (630 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | NT01EI_RS11945 |
| Coordinate (Strand) | 2645177..2645806 (+) |
| NCBI ID | WP_035608788.1 |
| RefSeq | NC_012779 |
| Uniprot ID | X2C2F0_EDWIC |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 6e-09 | 43..150 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-209 | MMKNKTIWGVIGCLLLSLTGCSSDPAKQQARRNTQWLYGSQAVLVNVQTAPDLNSYDGESHSLVLAVVQARSPDAFYQLIQTSELVGQVLQGGRPPDGILQVSRYALEPGHDFRLVLDRLQEAHQVGLIAAFYGLPVAQTSRLFDIPVKVTRSGILFPDWKAAPSPMALNLKLGNSGISEAVMNPVDSIPSASAPTASTPIRLSDISGQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9989 | pos: 20-21. LTG-CS. | MMKNKTIWGVIGCLLLSLTG |
Protein sequence: 210 a.a.
>T6CP003516 NC_012779:2645177-2645806 [Edwardsiella ictaluri 93-146] [TssJ]
MMKNKTIWGVIGCLLLSLTGCSSDPAKQQARRNTQWLYGSQAVLVNVQTAPDLNSYDGESHSLVLAVVQARSPDAFYQLI
QTSELVGQVLQGGRPPDGILQVSRYALEPGHDFRLVLDRLQEAHQVGLIAAFYGLPVAQTSRLFDIPVKVTRSGILFPDW
KAAPSPMALNLKLGNSGISEAVMNPVDSIPSASAPTASTPIRLSDISGQ*
MMKNKTIWGVIGCLLLSLTGCSSDPAKQQARRNTQWLYGSQAVLVNVQTAPDLNSYDGESHSLVLAVVQARSPDAFYQLI
QTSELVGQVLQGGRPPDGILQVSRYALEPGHDFRLVLDRLQEAHQVGLIAAFYGLPVAQTSRLFDIPVKVTRSGILFPDW
KAAPSPMALNLKLGNSGISEAVMNPVDSIPSASAPTASTPIRLSDISGQ*
Nucleotide sequence: 630 bp
>T6CP003516 NC_012779:2645177-2645806 [Edwardsiella ictaluri 93-146] [TssJ]
ATGATGAAGAATAAGACGATCTGGGGAGTCATCGGCTGCCTGCTGCTCAGTCTGACCGGCTGCTCATCCGATCCGGCAAA
GCAGCAGGCACGCCGCAATACGCAGTGGCTGTATGGCAGCCAGGCCGTTTTGGTTAATGTACAAACGGCCCCGGACCTGA
ACAGCTACGACGGCGAGTCGCACAGCCTGGTACTAGCGGTGGTTCAGGCCCGTTCACCTGACGCCTTTTATCAGTTAATC
CAAACATCCGAACTCGTCGGCCAGGTCTTGCAGGGCGGACGTCCCCCTGACGGAATTTTGCAGGTAAGCCGCTATGCGCT
GGAGCCCGGCCACGATTTCCGCCTGGTCCTGGATCGCCTGCAGGAGGCACACCAGGTGGGGCTCATCGCCGCTTTCTACG
GTCTACCGGTGGCGCAGACCTCCCGCCTGTTTGATATCCCGGTAAAAGTGACCCGCTCGGGGATACTGTTCCCGGACTGG
AAAGCCGCCCCCTCCCCTATGGCGTTGAACCTGAAACTGGGTAACAGCGGGATAAGCGAGGCGGTAATGAATCCGGTGGA
TAGCATCCCCTCGGCCAGCGCACCGACAGCATCGACGCCAATCCGGCTGAGCGATATCAGTGGACAATAG
ATGATGAAGAATAAGACGATCTGGGGAGTCATCGGCTGCCTGCTGCTCAGTCTGACCGGCTGCTCATCCGATCCGGCAAA
GCAGCAGGCACGCCGCAATACGCAGTGGCTGTATGGCAGCCAGGCCGTTTTGGTTAATGTACAAACGGCCCCGGACCTGA
ACAGCTACGACGGCGAGTCGCACAGCCTGGTACTAGCGGTGGTTCAGGCCCGTTCACCTGACGCCTTTTATCAGTTAATC
CAAACATCCGAACTCGTCGGCCAGGTCTTGCAGGGCGGACGTCCCCCTGACGGAATTTTGCAGGTAAGCCGCTATGCGCT
GGAGCCCGGCCACGATTTCCGCCTGGTCCTGGATCGCCTGCAGGAGGCACACCAGGTGGGGCTCATCGCCGCTTTCTACG
GTCTACCGGTGGCGCAGACCTCCCGCCTGTTTGATATCCCGGTAAAAGTGACCCGCTCGGGGATACTGTTCCCGGACTGG
AAAGCCGCCCCCTCCCCTATGGCGTTGAACCTGAAACTGGGTAACAGCGGGATAAGCGAGGCGGTAATGAATCCGGTGGA
TAGCATCCCCTCGGCCAGCGCACCGACAGCATCGACGCCAATCCGGCTGAGCGATATCAGTGGACAATAG