SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003505
Component protein type	TssJ
T6SS ID (Type)	T6SS00344 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon	chromosome
Sequence	Protein sequence (181 a.a.); Nucleotide sequence (543 bp)
Reference	[1] Sarris PF, Zoumadakis C, Panopoulos NJ, et al. Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011 Jan;11(1):157-66. doi: 10.1016/j.meegid.2010.09.006. Epub 2010 Oct 4. PMID: 20932940 [2] Hsieh PF, Lu YR, Lin TL, et al. Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization. J Infect Dis. 2019 Jan 29;219(4):637-647. doi: 10.1093/infdis/jiy534. PMID: 30202982

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KP1_RS11325
Coordinate (Strand)	2327935..2328477 (+)
NCBI ID	WP_004176417.1
RefSeq	NC_012731
Uniprot ID	A0A0H3GS07_KLEPH, A0A7U9CZW7_KLEPN, W1HXN0_KLEPN, A0A652Q1B5_9ENTR, A0A0E9Z527_KLEPN, A0A377ZH20_KLEPO, A0A1Y0Q0Z0_KLEPN
KEGG ID	kpnu:LI86_14515, kpv:KPNIH29_11490, kpy:KPNIH31_11120, kpg:KPNIH32_11920, kpb:FH42_03005, kpne:KU54_014580, kpw:KPNIH30_12080, kpm:KPHS_23190
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.9e-27	47..163

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-180	VLRISLTKTACLLPLLALCLSGCGLTQRVSEGTKSAFNAVFYKKINTLHLDFTAREALNTDARENHSLSEPVVVRIYQLKDRQTFDRLVYQQLLEEGDILLAADLLASRDVVIGPGGDASLNMPLEAEATFVAVVGLFRHPDTQRNTWKQVLAREELDPDKPRIFTAEHNQLRLRPEAAK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9981	pos: 22-23. LSG-CG.	VLRISLTKTACLLPLLALCLSG

Protein sequence: 181 a.a.

						>T6CP003505 NC_012731:2327935-2328477 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssJ]
VLRISLTKTACLLPLLALCLSGCGLTQRVSEGTKSAFNAVFYKKINTLHLDFTAREALNTDARENHSLSEPVVVRIYQLK
DRQTFDRLVYQQLLEEGDILLAADLLASRDVVIGPGGDASLNMPLEAEATFVAVVGLFRHPDTQRNTWKQVLAREELDPD
KPRIFTAEHNQLRLRPEAAK*					

Nucleotide sequence: 543 bp

						>T6CP003505 NC_012731:2327935-2328477 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssJ]
GTGTTACGTATAAGTTTGACTAAAACCGCCTGTCTGCTGCCCCTGCTTGCGCTTTGCCTGAGCGGATGCGGACTCACCCA
GCGCGTCTCGGAGGGAACAAAGTCTGCCTTCAACGCCGTTTTTTATAAGAAAATTAATACGCTGCATCTGGACTTTACCG
CGCGCGAAGCGCTCAACACCGATGCCAGAGAGAATCACTCGCTCTCTGAACCGGTGGTGGTGCGCATCTATCAGCTTAAA
GATCGCCAGACCTTTGACCGGCTGGTTTACCAGCAGCTGCTGGAGGAGGGGGATATTCTGCTCGCCGCCGATCTCCTCGC
CAGTCGCGATGTGGTGATCGGTCCCGGAGGGGACGCCAGTCTGAATATGCCGCTGGAAGCGGAGGCGACGTTCGTGGCGG
TTGTCGGCCTGTTCCGCCATCCGGACACACAAAGAAATACCTGGAAGCAGGTGCTGGCGCGTGAGGAATTGGATCCGGAC
AAGCCGCGCATTTTCACCGCCGAACACAACCAGCTACGGCTCAGGCCTGAAGCGGCGAAGTAA					