SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003504
Component protein type	TssG
T6SS ID (Type)	T6SS00344 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon	chromosome
Sequence	Protein sequence (362 a.a.); Nucleotide sequence (1086 bp)
Reference	[1] Sarris PF, Zoumadakis C, Panopoulos NJ, et al. Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011 Jan;11(1):157-66. doi: 10.1016/j.meegid.2010.09.006. Epub 2010 Oct 4. PMID: 20932940 [2] Hsieh PF, Lu YR, Lin TL, et al. Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization. J Infect Dis. 2019 Jan 29;219(4):637-647. doi: 10.1093/infdis/jiy534. PMID: 30202982

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KP1_RS11320
Coordinate (Strand)	2326872..2327957 (+)
NCBI ID	WP_004190503.1
RefSeq	NC_012731
Uniprot ID	A0A7U9CYK9_KLEPN, A0A652Q199_9ENTR, W9B806_KLEPN, A0A3F3B3D4_KLEPN
KEGG ID	kpnk:BN49_2478, kpy:KPNIH31_11115
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	5.7e-63	21..320

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-361	MGREAQPPHSRLTPRLEADLPRINFYRFCQLLEKRRPGQPLMGGTSHPADDPVRFYPHPGMGFPASELKAVEYDEADDSRPPVIRTTFMGLYGVDSPLPTAYLDDIAQHREGHEALQGLLDIFSHRIMTQFYRIWRKYSWPATFEPGGTDRLSQSLLGLAGLGIPGTTQHIASPASRFLALTGVLRQPGKTQQGIQALVTLLAPETTVRVSPYSLRPVAISQPLGFYGDDDFFLDGNTPLGDEAMDASSQLLVALTTDNPAEAQGWKPDGPLFRDFLILLRVYLGWRFRANITLTAPTRLLAVPPLGDEPFWLGMNGVLGVGEGESEGDIPQTFTTELGTYTGLQPATFLQGNRRVTYKFD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9812	-	-

Protein sequence: 362 a.a.

						>T6CP003504 NC_012731:2326872-2327957 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssG]
MGREAQPPHSRLTPRLEADLPRINFYRFCQLLEKRRPGQPLMGGTSHPADDPVRFYPHPGMGFPASELKAVEYDEADDSR
PPVIRTTFMGLYGVDSPLPTAYLDDIAQHREGHEALQGLLDIFSHRIMTQFYRIWRKYSWPATFEPGGTDRLSQSLLGLA
GLGIPGTTQHIASPASRFLALTGVLRQPGKTQQGIQALVTLLAPETTVRVSPYSLRPVAISQPLGFYGDDDFFLDGNTPL
GDEAMDASSQLLVALTTDNPAEAQGWKPDGPLFRDFLILLRVYLGWRFRANITLTAPTRLLAVPPLGDEPFWLGMNGVLG
VGEGESEGDIPQTFTTELGTYTGLQPATFLQGNRRVTYKFD*					

Nucleotide sequence: 1086 bp

						>T6CP003504 NC_012731:2326872-2327957 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssG]
ATGGGAAGAGAAGCACAGCCGCCGCATTCCCGGCTGACCCCGCGACTGGAGGCCGACCTGCCGCGGATCAATTTTTACCG
CTTCTGCCAGCTTCTGGAAAAACGGCGTCCCGGCCAACCGCTGATGGGGGGAACCAGCCATCCGGCTGACGATCCGGTGC
GTTTTTACCCGCATCCGGGAATGGGATTTCCCGCCAGCGAACTGAAAGCCGTGGAATACGATGAGGCCGATGACAGCAGG
CCGCCGGTCATTCGCACCACCTTTATGGGGTTGTACGGTGTCGATTCGCCGCTCCCGACGGCATATCTCGATGATATCGC
CCAGCACAGGGAAGGGCATGAAGCGCTGCAGGGCTTACTGGATATCTTCAGCCACCGGATCATGACGCAGTTTTACCGCA
TCTGGCGGAAATACTCCTGGCCCGCCACCTTTGAGCCCGGCGGCACCGATCGTCTCTCGCAGTCGCTACTGGGCCTGGCG
GGGCTGGGCATCCCCGGCACGACGCAGCATATCGCCTCGCCGGCGTCGCGCTTTCTGGCGCTAACCGGCGTGCTGCGTCA
GCCGGGCAAAACCCAGCAGGGGATCCAGGCGCTGGTCACCCTGCTGGCCCCGGAGACCACCGTCAGGGTGAGCCCGTACA
GCCTGCGTCCGGTGGCGATCAGCCAGCCGCTGGGGTTTTACGGCGATGACGACTTCTTCCTGGACGGCAATACGCCGCTG
GGTGACGAAGCGATGGATGCCAGCAGCCAGCTCCTCGTCGCCCTTACCACCGACAATCCCGCGGAGGCGCAGGGCTGGAA
GCCTGACGGCCCGCTGTTTCGGGACTTTCTCATCCTGCTGCGGGTATACCTCGGCTGGCGATTCCGGGCCAACATCACCC
TGACCGCGCCGACCCGACTGCTGGCTGTCCCGCCGCTGGGGGACGAGCCGTTCTGGCTCGGTATGAACGGCGTGCTGGGC
GTGGGTGAGGGCGAGAGTGAGGGCGATATTCCTCAGACCTTTACCACCGAGCTGGGTACCTATACGGGCCTGCAGCCTGC
CACCTTTCTACAAGGAAACCGACGTGTTACGTATAAGTTTGACTAA					