SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003497
Component protein type	TssB
T6SS ID (Type)	T6SS00344 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Reference	[1] Sarris PF, Zoumadakis C, Panopoulos NJ, et al. Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011 Jan;11(1):157-66. doi: 10.1016/j.meegid.2010.09.006. Epub 2010 Oct 4. PMID: 20932940 [2] Hsieh PF, Lu YR, Lin TL, et al. Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization. J Infect Dis. 2019 Jan 29;219(4):637-647. doi: 10.1093/infdis/jiy534. PMID: 30202982

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KP1_RS11220
Coordinate (Strand)	2298860..2299351 (+)
NCBI ID	WP_004176434.1
RefSeq	NC_012731
Uniprot ID	V0AWZ6_KLEPN, W1HNJ0_KLEPN, A0A264BV44_KLEVA, A0A652Q1X6_9ENTR, A0A6I4LTW9_9ENTR, A6T833_KLEP7, A0A3S4N0Z0_KLEAE, A0A4V0GXU9_KLEPN, A0A1Y0Q113_KLEPN
KEGG ID	kpn:KPN_01322, kpk:A593_21445, kpv:KPNIH29_11375, kpt:VK055_1107, kvq:SP68_00325, kpx:PMK1_03720, kpy:KPNIH31_11025
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	2.1e-52	7..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-163	MADTFQNEVPRARINLKLSLHTGGAQKKVELPLKLLTIGDFSHGKENRPLSEREKINVNKNNFNSVLSEFSPEVNLSVPNTLAGNGEEENVRLRFTDIKDFEPEQVARQIPQLRAMLAMRNLLRDLKSNLLDNATFRKELEAILKDPALSQELRDEMSALAPK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9936	-	-

Protein sequence: 164 a.a.

						>T6CP003497 NC_012731:2298860-2299351 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssB]
MADTFQNEVPRARINLKLSLHTGGAQKKVELPLKLLTIGDFSHGKENRPLSEREKINVNKNNFNSVLSEFSPEVNLSVPN
TLAGNGEEENVRLRFTDIKDFEPEQVARQIPQLRAMLAMRNLLRDLKSNLLDNATFRKELEAILKDPALSQELRDEMSAL
APK*					

Nucleotide sequence: 492 bp

						>T6CP003497 NC_012731:2298860-2299351 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssB]
ATGGCTGATACGTTCCAGAATGAAGTCCCCAGGGCGCGCATTAATCTCAAATTATCTCTTCATACCGGCGGCGCGCAAAA
GAAGGTCGAACTGCCGCTCAAACTGCTTACCATTGGCGATTTTAGTCATGGTAAGGAAAACCGGCCTTTATCGGAAAGAG
AAAAAATCAACGTCAATAAAAATAATTTTAACAGCGTACTTTCGGAATTTAGCCCGGAAGTTAACCTCTCGGTGCCGAAT
ACGCTGGCCGGCAATGGCGAAGAGGAGAACGTCAGACTGCGTTTTACCGACATCAAAGATTTTGAACCGGAGCAGGTTGC
CCGGCAAATTCCGCAACTCCGCGCCATGCTGGCGATGCGTAATTTATTACGCGATCTCAAATCCAATCTGCTCGATAACG
CCACTTTCAGAAAAGAGCTTGAGGCAATCCTGAAAGATCCGGCGCTGTCCCAGGAATTACGCGACGAAATGAGTGCGCTT
GCCCCGAAATAA					