Detailed information of component protein
Summary
| Component ID | T6CP003487 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00343  (Type i4b) |
| Strain | Burkholderia glumae BGR1 |
| Replicon | chromosome I |
| Sequence | Protein sequence (206 a.a.); Nucleotide sequence (618 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BGLU_RS01935 |
| Coordinate (Strand) | 399588..400205 (-) |
| NCBI ID | WP_012734477.1 |
| RefSeq | NC_012724 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 3.8e-29 | 55..173 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-205 | MTPFLSRFALPLAACALLAGCAAAVPLLGSAGSAALQMVGIGKPDVPDAQKPPRNLAVTLYAAPNLNAANDNRPLALVVRLYTLKDPTSFMQAPFDAFTDPAKEKATLGADLLSVREVTLIPGQHYNATEKVSREAQAFGVVALFRSPALQRWKLAFDPAKSEKSGIIIGLHNCAMTVTGGNVIAPQQGMPAQQLNMLSSVSCGG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.8848 | pos: 20-21. LAG-CA. | MTPFLSRFALPLAACALLAG |
Protein sequence: 206 a.a.
>T6CP003487 NC_012724:c400205-399588 [Burkholderia glumae BGR1] [TssJ]
MTPFLSRFALPLAACALLAGCAAAVPLLGSAGSAALQMVGIGKPDVPDAQKPPRNLAVTLYAAPNLNAANDNRPLALVVR
LYTLKDPTSFMQAPFDAFTDPAKEKATLGADLLSVREVTLIPGQHYNATEKVSREAQAFGVVALFRSPALQRWKLAFDPA
KSEKSGIIIGLHNCAMTVTGGNVIAPQQGMPAQQLNMLSSVSCGG*
MTPFLSRFALPLAACALLAGCAAAVPLLGSAGSAALQMVGIGKPDVPDAQKPPRNLAVTLYAAPNLNAANDNRPLALVVR
LYTLKDPTSFMQAPFDAFTDPAKEKATLGADLLSVREVTLIPGQHYNATEKVSREAQAFGVVALFRSPALQRWKLAFDPA
KSEKSGIIIGLHNCAMTVTGGNVIAPQQGMPAQQLNMLSSVSCGG*
Nucleotide sequence: 618 bp
>T6CP003487 NC_012724:c400205-399588 [Burkholderia glumae BGR1] [TssJ]
ATGACTCCGTTTCTCTCCCGCTTCGCGCTGCCGCTCGCGGCCTGCGCGCTTCTGGCAGGCTGCGCGGCGGCGGTGCCGCT
GCTCGGTTCGGCAGGCAGCGCCGCGCTGCAGATGGTCGGCATCGGCAAGCCCGATGTGCCCGACGCGCAGAAGCCGCCGC
GCAATCTGGCCGTGACCCTCTACGCCGCGCCGAACCTGAACGCCGCCAACGACAACCGGCCGCTCGCATTAGTAGTAAGG
CTCTACACGCTGAAGGATCCGACCTCGTTCATGCAGGCACCGTTCGACGCGTTCACCGATCCCGCCAAGGAGAAGGCCAC
GCTCGGCGCCGACCTGCTCAGCGTGCGCGAGGTGACGCTGATTCCGGGGCAGCACTACAACGCCACCGAGAAGGTCTCGC
GCGAGGCGCAGGCGTTCGGCGTCGTCGCGCTGTTTCGCTCGCCCGCGCTGCAACGCTGGAAGCTGGCATTCGATCCGGCG
AAATCGGAGAAATCCGGCATAATCATCGGCCTGCACAACTGCGCGATGACGGTGACGGGCGGTAACGTGATCGCGCCGCA
GCAGGGCATGCCGGCGCAGCAGCTCAATATGCTGTCGTCGGTCAGCTGCGGCGGCTGA
ATGACTCCGTTTCTCTCCCGCTTCGCGCTGCCGCTCGCGGCCTGCGCGCTTCTGGCAGGCTGCGCGGCGGCGGTGCCGCT
GCTCGGTTCGGCAGGCAGCGCCGCGCTGCAGATGGTCGGCATCGGCAAGCCCGATGTGCCCGACGCGCAGAAGCCGCCGC
GCAATCTGGCCGTGACCCTCTACGCCGCGCCGAACCTGAACGCCGCCAACGACAACCGGCCGCTCGCATTAGTAGTAAGG
CTCTACACGCTGAAGGATCCGACCTCGTTCATGCAGGCACCGTTCGACGCGTTCACCGATCCCGCCAAGGAGAAGGCCAC
GCTCGGCGCCGACCTGCTCAGCGTGCGCGAGGTGACGCTGATTCCGGGGCAGCACTACAACGCCACCGAGAAGGTCTCGC
GCGAGGCGCAGGCGTTCGGCGTCGTCGCGCTGTTTCGCTCGCCCGCGCTGCAACGCTGGAAGCTGGCATTCGATCCGGCG
AAATCGGAGAAATCCGGCATAATCATCGGCCTGCACAACTGCGCGATGACGGTGACGGGCGGTAACGTGATCGCGCCGCA
GCAGGGCATGCCGGCGCAGCAGCTCAATATGCTGTCGTCGGTCAGCTGCGGCGGCTGA