Detailed information of component protein


Summary

Component ID T6CP003091
Component protein type TssI
T6SS ID (Type) T6SS00303 experimental (Type i1)
Strain Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
Replicon chromosome
Sequence Protein sequence (704 a.a.); Nucleotide sequence (2112 bp)
Reference
[1] Blondel CJ, Yang HJ, Castro B, et al. Contribution of the type VI secretion system encoded in SPI-19 to chicken colonization by Salmonella enterica serotypes Gallinarum and Enteritidis. PLoS One. 2010 Jul 22;5(7):e11724. doi: 10.1371/journal.pone.0011724. PMID: 20661437
[2] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904
[3] Silva CA, Blondel CJ, Quezada CP, et al. Infection of mice by Salmonella enterica serovar Enteritidis involves additional genes that are absent in the genome of serovar Typhimurium. Infect Immun. 2012 Feb;80(2):839-49. doi: 10.1128/IAI.05497-11. Epub 2011 Nov 14. PMID: 22083712
[4] Blondel CJ, Jiménez JC, Leiva LE, et al. The type VI secretion system encoded in Salmonella pathogenicity island 19 is required for Salmonella enterica serotype Gallinarum survival within infected macrophages. Infect Immun. 2013 Apr;81(4):1207-20. doi: 10.1128/IAI.01165-12. Epub 2013 Jan 28. PMID: 23357385
[5] Pezoa D, Yang HJ, Blondel CJ, et al. The type VI secretion system encoded in SPI-6 plays a role in gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken. PLoS One. 2013 May 14;8(5):e63917. doi: 10.1371/journal.pone.0063917. Print 2013. PMID: 23691117
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) SG_RS05305
Coordinate (Strand) 1135593..1137704 (+)
NCBI ID 446025594
RefSeq NC_011274
Uniprot ID B5R7C4_SALG2
KEGG ID seg:SG1044
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.3e-73 29..327
Phage_base_V PF04717.14 7.7e-14 383..449
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-703 MSTGLRFTLEVDGLPPDALVVESFHLSQSLSTLFSLDISLVSQQLHNIEFAQVLEKPAHLKIWQGTEIQRRVNGIVTWFEQGENDGHQMLYSMKVRPPVWRAALRQNSRIFQNEDIKSILGTILQENGVTDWSPLFSEAHPAREFCVQYSETDYDFLARMAAEEGIFFYEEHAQTSDDQSLVLCDTVRFLPEAFEIPWNPNTRTEVSTPCVSQFRHSAQIRPSSVIGKDYTFKRPGWAGRFEHQGEHQDYQRTQYEVFDYPGRFKDGHGQNFARWQMEGWRNNAEVAQGKSRSPAIWPGRRIQLTEHPQASLNREWQVVSSDLHGSQPQAAGRSGSGTTLDNHFTVIPADRTWRPRPLPKPSVDGPQSAVVTGPEGEEIFCDEHGRVRVKFNWDRYNPANQDSSCWIRVVQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPSIMGHTYHQENRSPGSLPGTKTQMTIRSKTYKGSGFNELMFDDATGKERVYIHAQKNMNTEVLHNRTTDVTNNHAETIGNNQVIAVTNNQIQTIGVNQVEKVGSNQVIKVGANQVETVGIVRALNVGLAYQTTVGGIMNTSVALMQSSEVGIFKSLNVGVKYNVNVGRNASVNVGNSKTESTGKTAVYSAGEHLELVCGEARLVLTSDGGIFLNGKHIELQGVDSLNGDSKLISWNCGVSKKPPEASEQQDDPDPSDLIMY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9966 - -


  Protein sequence: 704 a.a.    

>T6CP003091 NC_011274:1135593-1137704 [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91] [TssI]
MSTGLRFTLEVDGLPPDALVVESFHLSQSLSTLFSLDISLVSQQLHNIEFAQVLEKPAHLKIWQGTEIQRRVNGIVTWFE
QGENDGHQMLYSMKVRPPVWRAALRQNSRIFQNEDIKSILGTILQENGVTDWSPLFSEAHPAREFCVQYSETDYDFLARM
AAEEGIFFYEEHAQTSDDQSLVLCDTVRFLPEAFEIPWNPNTRTEVSTPCVSQFRHSAQIRPSSVIGKDYTFKRPGWAGR
FEHQGEHQDYQRTQYEVFDYPGRFKDGHGQNFARWQMEGWRNNAEVAQGKSRSPAIWPGRRIQLTEHPQASLNREWQVVS
SDLHGSQPQAAGRSGSGTTLDNHFTVIPADRTWRPRPLPKPSVDGPQSAVVTGPEGEEIFCDEHGRVRVKFNWDRYNPAN
QDSSCWIRVVQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPSIMGHTYHQENRSPGSLPGTKTQMTIRSKTYKGSGFNE
LMFDDATGKERVYIHAQKNMNTEVLHNRTTDVTNNHAETIGNNQVIAVTNNQIQTIGVNQVEKVGSNQVIKVGANQVETV
GIVRALNVGLAYQTTVGGIMNTSVALMQSSEVGIFKSLNVGVKYNVNVGRNASVNVGNSKTESTGKTAVYSAGEHLELVC
GEARLVLTSDGGIFLNGKHIELQGVDSLNGDSKLISWNCGVSKKPPEASEQQDDPDPSDLIMY*

  Nucleotide sequence: 2112 bp    

>T6CP003091 NC_011274:1135593-1137704 [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91] [TssI]
ATGTCAACAGGATTACGTTTCACACTGGAGGTGGACGGTCTGCCACCGGATGCGCTGGTCGTGGAGTCCTTTCACCTGAG
CCAGTCCCTTTCCACGTTATTTTCCCTCGATATCTCGCTGGTCAGTCAGCAACTTCACAACATTGAGTTTGCCCAGGTAC
TGGAAAAACCGGCGCACCTGAAAATCTGGCAGGGCACGGAGATACAGCGCCGGGTGAACGGCATAGTGACCTGGTTTGAG
CAGGGGGAAAACGACGGACACCAGATGCTGTACAGCATGAAAGTTCGTCCGCCGGTGTGGCGCGCCGCGCTGCGCCAGAA
CTCGCGTATTTTCCAGAATGAAGACATAAAAAGCATTCTCGGGACGATACTGCAGGAAAACGGCGTGACGGACTGGAGCC
CGCTGTTCAGTGAGGCGCATCCGGCGCGGGAGTTCTGCGTGCAGTACAGCGAAACGGATTACGACTTTCTGGCGCGGATG
GCGGCGGAGGAAGGTATTTTCTTTTACGAGGAACATGCGCAGACCAGCGACGACCAGAGCCTGGTGCTGTGCGACACGGT
ACGTTTTCTGCCGGAAGCCTTTGAAATCCCCTGGAACCCGAACACCCGTACCGAGGTGAGCACGCCGTGCGTCAGCCAGT
TCCGCCACAGCGCGCAAATCCGCCCCTCTTCCGTCATCGGCAAGGACTACACCTTCAAACGCCCCGGCTGGGCGGGCCGC
TTTGAGCACCAGGGCGAACATCAGGATTACCAGCGCACGCAGTATGAGGTCTTTGATTATCCGGGGCGCTTCAAGGACGG
CCACGGTCAGAACTTCGCCCGCTGGCAGATGGAAGGCTGGCGTAATAATGCCGAAGTGGCGCAGGGGAAAAGCCGTTCGC
CGGCGATATGGCCGGGACGGCGTATTCAGCTGACGGAACACCCGCAGGCGAGCCTGAACCGGGAATGGCAGGTGGTGAGC
AGCGATTTACACGGCAGCCAGCCGCAGGCGGCGGGGCGCTCAGGTTCAGGGACAACGCTGGACAATCATTTTACGGTCAT
CCCGGCAGACAGAACATGGCGGCCACGCCCGCTGCCCAAACCATCAGTGGACGGCCCGCAGTCGGCGGTGGTCACCGGCC
CGGAAGGGGAAGAAATCTTCTGCGACGAACATGGCCGTGTACGGGTGAAATTTAACTGGGACCGGTATAACCCGGCGAAC
CAGGACAGCTCGTGCTGGATACGGGTGGTACAGGCGTGGGCGGGGCCGGGGTTCGGCAACCTGGCGATACCGCGCGTGGG
GCAGGAAGTGATAGTGGACTTCCTCAACGGCGACCCGGACCAGCCCAGCATCATGGGGCACACCTACCACCAGGAAAACC
GCTCACCGGGCAGCCTGCCGGGCACCAAAACGCAGATGACCATCCGCTCGAAAACCTATAAAGGCAGCGGGTTTAACGAA
CTCATGTTTGATGATGCGACAGGGAAGGAGCGCGTGTATATCCACGCGCAGAAGAATATGAATACCGAGGTGCTGCACAA
CCGCACCACCGATGTGACGAATAATCATGCTGAAACCATTGGTAATAACCAGGTGATCGCCGTTACCAATAACCAAATCC
AGACCATCGGCGTCAACCAGGTGGAGAAGGTGGGCAGTAACCAGGTGATCAAGGTGGGTGCCAATCAGGTGGAAACGGTG
GGGATTGTCCGCGCCCTGAATGTGGGGCTGGCATACCAGACTACCGTGGGCGGCATCATGAATACCTCGGTGGCGCTGAT
GCAGTCTTCTGAGGTCGGGATCTTCAAATCACTGAATGTGGGAGTGAAATACAACGTCAATGTCGGGCGTAATGCCAGCG
TTAACGTGGGCAACAGTAAAACCGAAAGTACCGGTAAAACGGCGGTGTATTCCGCAGGCGAGCATCTGGAACTGGTGTGT
GGTGAAGCCCGTCTGGTGCTGACGAGCGACGGCGGTATTTTTCTGAACGGCAAACATATTGAGTTGCAGGGCGTGGACAG
CTTAAACGGGGATTCAAAGCTGATCAGCTGGAACTGTGGGGTGAGCAAGAAGCCACCTGAAGCATCTGAACAGCAGGATG
ACCCCGATCCATCGGATCTGATCATGTACTGA