Detailed information of component protein


Summary

Component ID T6CP003083
Component protein type TssH
T6SS ID (Type) T6SS00303 experimental (Type i1)
Strain Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
Replicon chromosome
Sequence Protein sequence (888 a.a.); Nucleotide sequence (2664 bp)
Reference
[1] Blondel CJ, Yang HJ, Castro B, et al. Contribution of the type VI secretion system encoded in SPI-19 to chicken colonization by Salmonella enterica serotypes Gallinarum and Enteritidis. PLoS One. 2010 Jul 22;5(7):e11724. doi: 10.1371/journal.pone.0011724. PMID: 20661437
[2] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904
[3] Silva CA, Blondel CJ, Quezada CP, et al. Infection of mice by Salmonella enterica serovar Enteritidis involves additional genes that are absent in the genome of serovar Typhimurium. Infect Immun. 2012 Feb;80(2):839-49. doi: 10.1128/IAI.05497-11. Epub 2011 Nov 14. PMID: 22083712
[4] Blondel CJ, Jiménez JC, Leiva LE, et al. The type VI secretion system encoded in Salmonella pathogenicity island 19 is required for Salmonella enterica serotype Gallinarum survival within infected macrophages. Infect Immun. 2013 Apr;81(4):1207-20. doi: 10.1128/IAI.01165-12. Epub 2013 Jan 28. PMID: 23357385
[5] Pezoa D, Yang HJ, Blondel CJ, et al. The type VI secretion system encoded in SPI-6 plays a role in gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken. PLoS One. 2013 May 14;8(5):e63917. doi: 10.1371/journal.pone.0063917. Print 2013. PMID: 23691117
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) SG_RS05240
Coordinate (Strand) 1122020..1124683 (-)
NCBI ID 446536954
RefSeq NC_011274
Uniprot ID B5R7B4_SALG2
KEGG ID seg:SG1034
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 2.6e-37 606..767
AAA_lid_9 PF17871.3 4.1e-24 347..429
ClpB_D2-small PF10431.11 5.5e-09 773..850
AAA PF00004.31 7.5e-07 223..327
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-887 MIQIDLATLVKRLAPFAKQALEAAASECMSQQAAEITVAHVLLQMLATPRSDFRVIAERAEIGADDLRQALTVENYATARSSDSYPSFSPMLVEWLKEAWLLASAEMQQTELRGGVLLLALLHSPLRYLPPAAARLLTAINRDLLRQDFAGWTKESAESVILNADGQTASAIADTGDTLLARYAKNMTDDARQGRLDPVLCRDNEIDLMIDILCSRRKNNPVVVGEAGVGKSALIEGLALRIIAGQVPDKLRDTDIMTLDLGALQAGASVKGEFEKRFKGLMAEVIQSPKPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATTWSEYKKYFEKDSGLSAVYEQSHGVLIDDEALQAAATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPRQISALTPLRQQCEAEIRQLEREIRIGLRSDTSRLNEVLAQYDETLSELDALEAAWRQQQTLVQEIIALRKGLLDETDATGNDETATPETEAVGSDDESVTAPPEAEPEISPAARLAQLTAELDALHHTQLLVSPHVDKKQIASVIAEWTGVPLNRLSQNEMSVITDLPQWLGDTIKGQALAIKHLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNVVFFLTSNLGYQVIVEHADNPEAMQEALYPVLADFFKPALLARMEVVPYLPLSRETLAVIIAGKLARLDNVLRARFGAEVVIEPEVTDEIMQRVTRAENGARMLESVIDGEMLPPLSLLLLQKMAANIAIARITLGVANGAFTADVEDVPDEDTQPVAEAEDEAVL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9804 - -


  Protein sequence: 888 a.a.    

>T6CP003083 NC_011274:c1124683-1122020 [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91] [TssH]
MIQIDLATLVKRLAPFAKQALEAAASECMSQQAAEITVAHVLLQMLATPRSDFRVIAERAEIGADDLRQALTVENYATAR
SSDSYPSFSPMLVEWLKEAWLLASAEMQQTELRGGVLLLALLHSPLRYLPPAAARLLTAINRDLLRQDFAGWTKESAESV
ILNADGQTASAIADTGDTLLARYAKNMTDDARQGRLDPVLCRDNEIDLMIDILCSRRKNNPVVVGEAGVGKSALIEGLAL
RIIAGQVPDKLRDTDIMTLDLGALQAGASVKGEFEKRFKGLMAEVIQSPKPVILFIDEAHTLIGAGNQQGGLDISNLLKP
ALARGELKTIAATTWSEYKKYFEKDSGLSAVYEQSHGVLIDDEALQAAATLSERYLSGRQLPDKAIDVLDTACARVAINL
SSPPRQISALTPLRQQCEAEIRQLEREIRIGLRSDTSRLNEVLAQYDETLSELDALEAAWRQQQTLVQEIIALRKGLLDE
TDATGNDETATPETEAVGSDDESVTAPPEAEPEISPAARLAQLTAELDALHHTQLLVSPHVDKKQIASVIAEWTGVPLNR
LSQNEMSVITDLPQWLGDTIKGQALAIKHLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLT
TINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCK
NVVFFLTSNLGYQVIVEHADNPEAMQEALYPVLADFFKPALLARMEVVPYLPLSRETLAVIIAGKLARLDNVLRARFGAE
VVIEPEVTDEIMQRVTRAENGARMLESVIDGEMLPPLSLLLLQKMAANIAIARITLGVANGAFTADVEDVPDEDTQPVAE
AEDEAVL*

  Nucleotide sequence: 2664 bp    

>T6CP003083 NC_011274:c1124683-1122020 [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91] [TssH]
ATGATCCAGATTGACTTAGCCACCCTGGTAAAACGGCTTGCTCCCTTCGCGAAACAGGCGCTGGAAGCGGCGGCGTCGGA
GTGTATGAGCCAGCAGGCGGCAGAAATTACCGTCGCGCATGTGCTGCTACAGATGCTGGCGACCCCGCGCAGCGACTTTC
GGGTGATTGCCGAACGTGCGGAGATCGGCGCCGATGATTTACGCCAGGCGCTGACGGTGGAAAATTACGCCACCGCCCGT
TCATCCGACAGCTACCCGTCGTTTTCCCCGATGCTGGTTGAATGGCTGAAAGAGGCCTGGCTGCTGGCCTCCGCGGAAAT
GCAGCAGACCGAACTGCGCGGCGGCGTGCTGCTGCTCGCCCTGCTGCATTCGCCGTTGCGCTACCTGCCGCCCGCCGCCG
CCCGGTTGCTGACCGCCATCAACCGCGACCTGCTCCGGCAGGATTTTGCCGGGTGGACGAAGGAGTCCGCCGAATCGGTG
ATCCTGAATGCGGACGGACAGACCGCCAGCGCCATAGCCGATACCGGCGACACCCTGCTGGCCCGCTATGCCAAAAACAT
GACCGACGATGCCCGGCAGGGGCGGCTTGATCCGGTGCTGTGCCGCGATAACGAAATTGACCTGATGATCGATATCCTCT
GCAGCCGCCGTAAAAATAATCCGGTGGTGGTGGGCGAAGCGGGCGTGGGCAAAAGCGCGCTGATTGAAGGGCTGGCGCTG
CGCATTATTGCCGGTCAGGTGCCGGACAAACTGCGCGATACCGATATTATGACGCTGGATCTCGGCGCATTACAGGCGGG
CGCGTCGGTGAAAGGTGAATTTGAGAAGCGCTTTAAGGGGCTGATGGCGGAAGTCATTCAGTCGCCGAAACCCGTCATCC
TGTTTATCGACGAAGCCCACACGCTGATTGGCGCGGGCAACCAGCAGGGCGGGCTGGATATCTCCAACCTGCTCAAACCG
GCACTGGCGCGCGGCGAGCTGAAAACCATCGCCGCCACCACCTGGAGCGAATACAAAAAATACTTCGAGAAAGACTCCGG
CCTGTCGGCGGTGTATGAGCAGTCCCACGGCGTGCTGATTGACGATGAAGCCTTACAGGCCGCTGCGACGTTGAGCGAGC
GCTACCTGTCAGGCCGCCAGTTGCCGGATAAGGCCATTGATGTGCTGGATACCGCCTGCGCCCGCGTGGCGATTAACCTC
TCCTCGCCGCCGAGACAAATCTCGGCGCTGACCCCCCTGCGCCAGCAGTGTGAAGCGGAAATCCGCCAGCTTGAGCGTGA
GATCCGTATTGGCCTGCGGTCGGACACCTCACGGCTAAACGAGGTTCTGGCGCAGTATGACGAAACGCTGAGCGAACTGG
ATGCGCTGGAGGCCGCGTGGCGCCAGCAGCAGACGCTGGTGCAGGAGATTATCGCGCTGCGTAAGGGGCTGCTGGACGAA
ACGGATGCTACCGGCAACGACGAAACCGCAACGCCTGAAACAGAGGCGGTGGGCAGTGACGATGAATCCGTCACAGCGCC
GCCGGAAGCGGAACCGGAAATTTCGCCAGCCGCCCGCCTCGCGCAGCTCACCGCCGAACTGGACGCCCTGCACCACACGC
AGCTGCTGGTCTCCCCACACGTCGATAAAAAACAGATAGCCAGCGTGATTGCCGAATGGACCGGCGTGCCGCTTAACCGC
CTGTCACAAAACGAAATGTCGGTCATCACCGACCTGCCGCAGTGGCTGGGCGACACCATTAAAGGCCAGGCGCTGGCGAT
TAAGCACCTGCACAAACACCTGCTGACCGCCCGCGCCGATCTGCGCCGTCCGGGACGCCCGCTCGGCGCGTTCCTGCTGG
CCGGTCCCAGCGGTGTCGGCAAAACCGAAACCGTGCTGCAACTGGCCGAACTGCTCTACGGCGGTCGCCAGTACCTGACC
ACCATCAATATGTCGGAGTTTCAGGAGAAACACACCGTTTCACGACTGATTGGTTCGCCGCCGGGCTATGTCGGCTACGG
CGAGGGCGGCGTGCTGACCGAAGCGATTCGTCAGAAACCGTACTCCGTGGTGCTGCTCGACGAGGTGGAGAAAGCCCACC
CGGACGTGCTCAACCTGTTCTACCAGGCGTTCGACAAGGGCGAGATGGCCGATGGCGAAGGGCGTCTTATCGACTGTAAA
AATGTGGTGTTCTTCCTCACCTCCAACCTCGGCTATCAGGTGATTGTCGAACATGCCGACAACCCGGAAGCCATGCAGGA
AGCGCTGTACCCGGTACTGGCGGACTTCTTTAAACCGGCGCTGCTGGCGCGTATGGAAGTGGTGCCGTACCTGCCGTTAT
CCAGAGAAACGCTTGCCGTCATTATCGCCGGGAAGCTGGCGCGGCTGGACAACGTACTGCGCGCCCGCTTCGGCGCGGAG
GTGGTGATTGAGCCGGAGGTGACCGACGAAATCATGCAGCGCGTCACCCGCGCCGAAAACGGCGCGCGGATGCTGGAGTC
GGTCATCGACGGCGAGATGCTGCCGCCGCTCTCCCTGCTGCTGTTGCAGAAAATGGCGGCGAATATCGCCATTGCCCGTA
TCACGCTGGGCGTGGCAAACGGCGCGTTTACCGCGGATGTGGAGGATGTGCCGGATGAGGATACTCAGCCCGTAGCGGAA
GCAGAGGACGAAGCCGTTCTATGA