SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003073
Component protein type	TssL
T6SS ID (Type)	T6SS00302 (Type i1)
Strain	Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853
Replicon	chromosome
Sequence	Protein sequence (253 a.a.); Nucleotide sequence (759 bp)
Reference	[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904 [2] Pezoa D, Blondel CJ, Silva CA, et al. Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts. Vet Res. 2014 Jan 9;45:2. doi: 10.1186/1297-9716-45-2. PMID: 24405577

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	SED_RS06270
Coordinate (Strand)	1216476..1217234 (-)
NCBI ID	446266123
RefSeq	NC_011205
Uniprot ID	M7RK81_SALDU, B5F2M5_SALA4, B5R7B5_SALG2, A0A2T9HRP8_SALET
KEGG ID	seg:SG1035, sea:SeAg_B1103
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	2.9e-55	31..231

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-210	MDELSLPPLPGYEREGQRQYQLALRGNSLNPMIDAATPLLGMVMRVSGMNSHAMPEHLFTQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLMREPKRYQDLLEFLYICFSLGFRGRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLIKR
Transmembrane helix	211-233	LTIRHVLCGGIVVLALFYLFYLL
Inside	234-252	RLDSQTQDILHQLNRLLAR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8852	-	-

Protein sequence: 253 a.a.

						>T6CP003073 NC_011205:c1217234-1216476 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TssL]
MDELSLPPLPGYEREGQRQYQLALRGNSLNPMIDAATPLLGMVMRVSGMNSHAMPEHLFTQVVTDVQAVEQLLQEQGYEP
GVIISFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLMREPKRYQDLLEFLYICFSLGFRGR
YKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLIKRLTIRHVLCGGIVVLALFYLFYLLRLDSQTQ
DILHQLNRLLAR*					

Nucleotide sequence: 759 bp

						>T6CP003073 NC_011205:c1217234-1216476 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TssL]
ATGGATGAGCTGTCACTGCCCCCTTTACCCGGATACGAGAGAGAAGGCCAGCGCCAGTATCAGCTGGCGCTGCGCGGCAA
CAGCCTGAACCCGATGATCGATGCCGCCACCCCGCTGCTGGGGATGGTGATGCGCGTTTCGGGCATGAACAGCCATGCCA
TGCCTGAACACCTGTTCACCCAGGTGGTGACGGATGTGCAGGCGGTGGAGCAGTTACTCCAGGAGCAGGGCTATGAGCCT
GGGGTGATTATCTCGTTTCGTTACATCCTGTGTACCTTTATTGACGAGGCGGCGCTGGGGAATGGCTGGTCGAACAAAAA
CGAGTGGATCAAGCAGTCGCTGCTGGTGCATTTCCATAACGAAGCCTGGGGCGGTGAAAAGGTTTTTATCCTGCTGGAGC
GCCTGATGCGCGAACCCAAACGCTACCAGGATCTGCTGGAATTTTTGTACATCTGCTTTTCGCTGGGCTTTCGCGGGCGT
TATAAAGTCGCCGTCCAGGACCAGGGGGAGTTTGAGCAGATATACCGCCGCCTGTATCACGTCCTGCATAAGCTGCGCGG
CGATGCGCCCTTTCCGCTGCTGCACCAGGATAAAAAAACCCAGGGCGGGCGCTATCAGCTGATTAAACGGCTGACCATCC
GGCATGTTCTGTGCGGCGGCATCGTCGTGCTGGCGCTGTTTTATCTGTTTTATCTGCTGCGTCTGGACAGTCAGACGCAG
GACATTCTTCATCAGTTAAACCGGCTGCTTGCCAGGTAA					